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Identification of the DNA damage-responsive elements of therhp51 + gene, arecA andRAD51 homolog from the fission yeastSchizosaccharomyces pombe (1996)

Jang, Y. K. ; Jin, Y. H. ; Shim, Y. S. ; [weitere]

Springer

Molecular genetics and genomics 251 (1996), S. 167-175

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ISSN: 1617-4623

Schlagwort(e): Schizosaccharomyces pombe ; DNA-damage inducibility ; Damage-responsive element ; Upstream activating sequence

Quelle: Springer Online Journal Archives 1860-2000

Thema: Biologie

Notizen: Abstract TheSchizosaccharomyces pombe rhp51 + gene encodes a recombinational repair protein that shares significant sequence identities with the bacterial RecA and theSaccharomyces cerevisiae RAD51 protein. Levels ofrhp51 + mRNA increase following several types of DNA damage or inhibition of DNA synthesis. Anrhp51::ura4 fusion gene was used to identify the cis-acting promoter elements involved in regulatingrhp51 + expression in response to DNA damage. Two elements, designated DRE1 and DRE2 (fordamage-responsiveelement), match a decamer consensus URS (upstream repressing sequence) found in the promoters of many other DNA repair and metabolism genes fromS. cerevisiae. However, our results show that DRE1 and DRE2 each function as a UAS (upstream activating sequence) rather than a URS and are also required for DNA-damage inducibility of the gene. A 20-bp fragment located downstream of both DRE1 and DRE2 is responsible for URS function. The DRE1 and DRE2 elements cross-competed for binding to two proteins of 45 and 59 kDa. DNase I footprint analysis suggests that DRE1 and DRE2 bind to the same DNA-binding proteins. These results suggest that the DRE-binding proteins may play an important role in the DNA-damage inducibility ofrhp51 + expression.

Materialart: Digitale Medien

URL: http://dx.doi.org/10.1007/BF02172915

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Digitale Medien

Atomic Xα calculations based on EHFS(α) = Eexp (1980)

Tseng, T. J. ; Hong, S. H. ; Whitehead, M. A.

New York, NY [u.a.] : Wiley-Blackwell

Journal of Computational Chemistry 1 (1980), S. 88-93

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ISSN: 0192-8651

Schlagwort(e): Computational Chemistry and Molecular Modeling ; Biochemistry

Quelle: Wiley InterScience Backfile Collection 1832-2000

Thema: Chemie und Pharmazie , Informatik

Notizen: The total energies and one-electron energies for first- and second-row atoms were calculated by using the Hartree-Fock and the Hartree-Fock-Slater Hamiltonian with Xα orbitals, ui(αexp); α was parametrized from EHFS(αexp) = Eexp. The EHF(αexp) total energies are always higher than the Hartree-Fock energies for the atoms. The relation of the calculated ionization potential to the experimental ionization potential depends on the α used to define ui(α), αexp, or αHF.

Zusätzliches Material: 1 Ill.