ISSN:
1432-1041
Schlagwort(e):
Conjugate-cleavage
;
drug metabolism
;
gas chromatography
;
phenobarbital
;
urinary excretion
Quelle:
Springer Online Journal Archives 1860-2000
Thema:
Chemie und Pharmazie
,
Medizin
Notizen:
Summary A method for the quantitative determination of phenobarbital and free and conjugatedp-hydroxyphenobarbital in urine samples is described. The method includes initial extraction, purification on a small chromatographic column and finally determination by gas chromatography. The barbituric acids are methylated by trimethylanilinium hydroxide which serves as a “flash heater” methylating agent. The conjugate ofp-hydroxyphenobarbital, which appears to be a glucuronide, is hydrolysed with hydrochloric acid.
Materialart:
Digitale Medien
URL:
http://dx.doi.org/10.1007/BF00614013
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