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  • 1
    Electronic Resource
    Electronic Resource
    Springer
    Archives of microbiology 100 (1974), S. 341-350 
    ISSN: 1432-072X
    Keywords: Coryneform Hydrogen Bacterium ; Autotrophic Growth ; Entner-Doudoroff Pathway ; Hydrogenase ; Slime Formation ; Corynebacterium autotrophicum
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract 1. Corynebacterium autotrophicum strain 7 C was isolated from an enrichment culture designed for propane oxidizing bacteria. The cells are Grampositive, immotile, short, irregularly formed rods. The colonies are yellow-pigmented and slimy. The yellow pigmentation is due to carotenoids. 2. Growth occurs either autotrophically in mineral medium under an atmosphere of 70% H2+20% O2+10% CO2 or heterotrophically with fructose or many organic acids as substrates. 3. The hexoses and gluconate are degraded via the Entner-Doudoroff pathway. 6-Phosphogluconate dehydrogenase is not detectable. 4. A NAD reducing hydrogenase has not been detected; the hydrogenase is localized in the particle fraction of the crude extract and reduces methylene blue. The specific activity of hydrogenase in the crude extract of autotrophically grown cells is 2400 μl H2/mg protein · hr. During growth on fructose the enzyme is constitutively formed (1200 μl H2/mg protein · hr). 5. The utilization of fructose was suppressed by hydrogen. The inhibitory effect was significant, when either fully adapted or autotrophically grown cells were exposed to a hydrogen containing atmosphere.
    Type of Medium: Electronic Resource
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  • 2
    Electronic Resource
    Electronic Resource
    Springer
    Archives of microbiology 100 (1974), S. 351-361 
    ISSN: 1432-072X
    Keywords: Selection of Slime-Free Mutant ; Coryneform Hydrogen Bacterium ; Capsular Material ; Bacterial Polysaccharide ; Chemostat Selection ; Selective Advantage
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract A slime-free mutant was isolated from Corynebacterium autotrophicum strain 7 C. The mutant appeared in a substrate (fructose) limited chemostat culture. No significant differences between wildtype and mutant were detected with respect to pigmentation, to growth rate, and respiratory rate at varying substrate (fructose) concentrations. During growth in chemostat culture the wildtype/mutant ratio present in the inoculum was maintained for weeks. The results do not explain the selection of the mutant in the substrate limited chemostat culture. The slime is a polysaccharide containing glucose, mannose and galactose; the uronic acid present was not identified. Copious amounts are produced from fructose, less from lactate or during autotrophic growth. The slime is not reutilized.
    Type of Medium: Electronic Resource
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