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  • 1
    Electronic Resource
    Electronic Resource
    In situ hybridization of muscle mitochondrial mRNA in mitochondrial myopathies (1990)
    Springer
    Acta neuropathologica 81 (1990), S. 1-6 
    Details
    ISSN: 1432-0533
    Keywords: In situ hybridization ; Mitochondrial DNA ; Mitochondrial myopathy ; Ophthalmoplegia ; Cytochromec oxidase
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary To determine whether a mitochondrial mRNA deficiency exists in mitochondrial myopathies, muscle biopsies from a patient with chronic progressive external ophthalmoplegia (CPEO) and a patient with mitochondrial myopathy, encephalopathy, lactic acidosis and stroke-like episodes (MELAS) were studied using in situ hybridization. Histochemistry and immunohistochemistry were performed along with hybridization. Hybridization reactions were widely distributed over the sarcoplasm of all muscle fibers in the patient with MELAS. In the patient with CPEO, 80% of the fibers showed a marked decrease in density of autoradiographic grains. This marked decrease corresponded to the histochemical and immunohistochemical findings of a very weak staining of cytochromec oxidase (CCO). The isotope-labeled cDNA probe used in in situ hybridization in this study complements a part of subunit I of CCO and a part of subunit II of complex I in the mitochondrial gene. Our results suggest a defect in the mRNA in this CPEO patient.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00662630
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  • 2
    Electronic Resource
    Electronic Resource
    Decreased activity of cytochromec oxidase in the macular mottled mouse: an immuno-electron microscopic study (1989)
    Springer
    Acta neuropathologica 77 (1989), S. 465-471 
    Details
    ISSN: 1432-0533
    Keywords: Menkes kinky hair syndrome ; Macular mottled mouse ; Mitochondrion ; Cytochromec oxidase ; Gold-labeling immuno-electron microscopy
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary The macular mottled mouse is a murine model of the kinky hair syndrome, characterized by a deficiency in copper transport. Cytochromec oxidase (CCO), a respiratory enzyme, is located in the inner mitochondrial membrane and consists of seven subunits, along with copper and iron. Biochemical and histochemical findings indicated that CCO activity was decreased in the cerebellum of the macular mottled mice but not in that of the controls. Immunocytochemical analysis, using anti-CCO and anti-complex III rabbit sera, revealed that CCO in the macular mottled mice was stained more weakly than that in the controls. Immuno-electron microscopic examination of CCO and complex III, using a method of gold labeling, was also performed. In the control mice, a high concentration of gold particles present over CCO and complex III could be seen in the inner mitochondrial membrane. The number of CCO-labeled gold particles was remarkably less, however, in the macular mottled mice, while no significant difference was found in the labeling of complex III between the two groups. It may concluded that the very low CCO enzyme content in the macular mottled mouse results not only from a copper transport disorder but also from a CCO protein synthesis disorder which impairs the localization of CCO protein in the cerebellum.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00687247
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    Paper (German National Licenses)
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  • 3
    Electronic Resource
    Electronic Resource
    Ultrastructural research on cell wall regeneration by cultured pollen protoplasts of Lilium longiflorum (1988)
    Springer
    Sexual plant reproduction 1 (1988), S. 36-45 
    Details
    ISSN: 1432-2145
    Keywords: Pollen protoplast ; Pollen tube ; Lilium longiflorum ; Cell wall regeneration
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Protoplasts from pollen grains of Lilium longiflorum regenerate amorphous cellulosic cell walls in culture, during which some precursors of cellulose are polymerized, thus producing progressively harder cellulosic cell walls as the period of culture continues. It is presumed that the components of the cell wall regenerated during 1 week in culture differ from those of the intine of the pollen grain wall. The regenerated cell wall is formed by means of large smooth vesicles; in addition, numerous coated vesicles and pits aid in wall regeneration. The pollen tube that germinates from the 8-day-old cultured protoplast has numerous Golgi bodies and many vesicles which build the pollen tube wall. The tube wall has two layers just like a normal pollen tube wall.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00227021
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  • 4
    Electronic Resource
    Electronic Resource
    Immunocytochemical localization of callose in the germinated pollen ofCamellia japonica (1996)
    Springer
    Protoplasma 194 (1996), S. 133-139 
    Details
    ISSN: 1615-6102
    Keywords: Camellia japonica ; Callose ; Pollen tube ; Callose plug ; Golgi vesicle ; Immuno-localization
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary A polyclonal antibody against β-1,3-glucan, callose, extracted from the pollen tube wall ofCamellia japonica was raised in mice and, using it as a probe, the localization of callose in the germinated pollen was studied. By confocal laser scanning microscopy, callose was found in the tip region of the pollen tube and the tube wall; the immuno-fluorescence in the tube wall was less toward the base of the tube. In contrast, the tip region did not fluoresce although the whole of the tube wall did strongly with aniline blue, the specific dye for callose. Immuno-electron microscopy showed that callose was also found in Golgi vesicles which concentrated in the tip region of the pollen tube, the inner layer of the tube wall, callose plugs, and Golgi vesicles in the pollen grain. Immuno-gold labeling was often detected on the fibrous structures in Golgi vesicles and callose plugs. Based on these results, the participation of Golgi vesicles in the formation of the tube wall and callose plugs was discussed.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01882021
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  • 5
    Electronic Resource
    Electronic Resource
    Effects of myrmicacin (β-hydroxydecanoic acid) on protoplasmic movement and ultrastructure ofCamellia japonica pollen (1981)
    Springer
    Protoplasma 105 (1981), S. 341-345 
    Details
    ISSN: 1615-6102
    Keywords: Golgi-vesicles ; Myrmicacin ; Pollen tube ; Protoplasmic movement
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary While tube elongation of growing pollen ofCamellia japonica was stopped by treatment with 50–100 ppm of myrmicacin, protoplasmic movement in the pollen tube still continued. However, higher concentration (200 ppm) of the inhibitor arrested the movement. The vesicles containing membrane substances disappeared at the tip of the tube of the growth-inhibited pollen. Removal of the inhibitor resulted in the reappearance of the vesicles at the tip region and tube elongation was restored.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01279231
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    Paper (German National Licenses)
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  • 6
    Electronic Resource
    Electronic Resource
    Infrared spectroscopic studies of polymer transitions. III. Effects of sodium ion of glass transitions in styrene-based ionomers (1974)
    New York : Wiley-Blackwell
    Journal of Polymer Science: Polymer Physics Edition 12 (1974), S. 633-633 
    Details
    ISSN: 0098-1273
    Keywords: Physics ; Polymer and Materials Science
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology , Physics
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/pol.1974.180120315
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  • 7
    Electronic Resource
    Electronic Resource
    Low-temperature dielectric relaxation in poly(4,4′-oxydiphenylene pyromellitimide) (1984)
    New York : Wiley-Blackwell
    Journal of Polymer Science: Polymer Physics Edition 22 (1984), S. 1399-1405 
    Details
    ISSN: 0098-1273
    Keywords: Physics ; Polymer and Materials Science
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology , Physics
    Notes: The effect of end groups on the low-temperature dielectric relaxation in poly(4,4′-oxydiphenylene pyromellitimide) has been studied using three kinds of samples with different content of anhydride end groups. The magnitude of the dielectric loss peak, designated here the V peak, which appears near -40°C at 1 kHz, depends on the content of anhydride end groups. If a sample with few anhydride end groups is allowed to absorb water, a loss peak appears near the temperature region of the V peak, but it disappears after subsequent heating. In contrast, a sample having a larger amount of anhydride end groups exhibits a large V peak which remains even after further heating. We conclude that the —O—portion of the anhydride end groups is responsible for the V peak, though there may be an additional contribution from absorbed water.
    Additional Material: 5 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/pol.1984.180220804
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  • 8
    Electronic Resource
    Electronic Resource
    Physical properties and structure of silk. VIII. Effect of casting temperature on conformation of wild silk fibroin films (1985)
    New York : Wiley-Blackwell
    Journal of Polymer Science: Polymer Physics Edition 23 (1985), S. 227-229 
    Details
    ISSN: 0098-1273
    Keywords: Physics ; Polymer and Materials Science
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology , Physics
    Additional Material: 1 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/pol.1985.180230120
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