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  • Spinacia  (10)
  • Cytogenetics  (5)
  • Key words Bone neoplasms  (4)
  • 1
    Electronic Resource
    Electronic Resource
    Chromosome analyses in patients with myelodysplastic syndromes: Correlation with bone marrow histopathology and prognostic significance (1992)
    Springer
    Virchows Archiv 421 (1992), S. 47-52 
    Details
    ISSN: 1432-2307
    Keywords: Myelodysplastic syndrome ; Myelofibrosis ; Cytogenetics ; Histopathology ; Bone marrow biopsy
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary Chromosome analyses of bone marrow and peripheral blood cells were performed in a total of 51 patients with myelodysplastic syndromes (MDS) simultaneously with histopathological examination of resinembedded bone marrow biopsies. Diagnosis of MDS was established by histopathology according to the French-American-British (FAB) classification, and reassessed by haematological data and clinical course. Clonal karyotypic changes were found in 30 of the 51 patients (59%): in 15 of 19 (79%) patients with refractory anaemia, 7 of 11 (64%) with refractory anaemia and excess of blasts (RAEB), 6 of 10 (60%) with RAEB in transformation, and 2 of 11 (18%) with chronic myelomonocytic leukaemia. The following three features of the histopathology revealed positive correlations with karyotype abnormalities: all cases of myelofibrosis in MDS (7/51) were accompanied by chromosome aberrations, microforms of megakaryocytes with reduced nuclear lobulation were observed in 18 of 30 cases with karyotype changes, and hypocellularity of haematopoiesis was associated with aberrations of chromosome 7 in 2 of 4 cases. No positive correlations were revealed between abnormal karyotypes and the transformation to acute leukaemia. The survival times were significantly decreased in patients with complex (3 and more) karyotype changes, when compared with patients with single (1–2) chromosome aberrations or normal karyotype, independently of the FAB classification.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01607138
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  • 2
    Electronic Resource
    Electronic Resource
    Trisomy 1 and 8 occur frequently in hepatocellular carcinoma but not in liver cell adenoma and focal nodular hyperplasia. A fluorescence in situ hybridization study (1995)
    Springer
    Virchows Archiv 427 (1995), S. 373-378 
    Details
    ISSN: 1432-2307
    Keywords: Hepatocellular carcinoma ; Cholangiocellular carcinoma ; Cytogenetics ; Chromosome 1 ; Fluorescence in situ hybridization (FISH)
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract Conventional cytogenetic studies revealed gains and structural aberrations of chromosome 1 to be the most consistent chromosomal aberrations in hepatocellular carcinoma (HCC). We investigated touch preparations of eight HCC, five cholangiocellular carcinomas (CCC), five liver cell adenomas (LCA), four focal nodular hyperplasias (FNH) as well as nine specimens of normal liver tissue using fluorescence in situ hybridization (FISH) with centromere specific probes for chromosomes 1 and 8. Polysomies of chromosome 1, especially trisomy 1, were found in five of eight HCC and four of five CCC but in no normal liver tissue or benign tumour. Only three of seven cases of HCC revealed trisomy 8 whereas the five benign liver tumours and all normal liver tissues examined had disomy 8. Our results confirm conventional cytogenetic findings in terms of chromosome 1 aberrations in HCC although they are not specific for these types of malignant liver tumours. Since α-satellite probes were used in our study, only gains or losses including the centromeric regions of the chromosomes 1 and 8 could be detected. Nevertheless, our findings suggest that FISH may help in the differential diagnosis of malignant versus benign neoplasms of the liver.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00199385
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  • 3
    Electronic Resource
    Electronic Resource
    The role of monovalent cations for photosynthesis of isolated intact chloroplasts (1980)
    Springer
    Planta 149 (1980), S. 170-175 
    Details
    ISSN: 1432-2048
    Keywords: Cations and photosynthesis ; Chloroplast (low-salt effects) ; Light activation (photosynthesis enzymes) ; Photosynthesis ; Spinacia
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The role of monovalent cations in the photosynthesis of isolated intact spinach chloroplasts was investigated. When intact chloroplasts were assayed in a medium containing only low concentrations of mono- and divalent cations (about 3 mval l-1), CO2-fixation was strongly inhibited although the intactness of chloroplasts remained unchanged. Addition of K+, Rb+, or Na+ (50–100 mM) fully restored photosynthesis. Both the degree of inhibition and restoration varied with the plant material and the storage time of the chloroplasts in “low-salt” medium. In most experiments the various monovalent cations showed a different effectiveness in restoring photosynthesis of low-salt chloroplasts (K+〉Rb+〉Na+). Of the divalent cations tested, Mg2+ also restored photosynthesis, but to a lesser extent than the monovalent cations. In contrast to CO2-fixation, reduction of 3-phosphoglycerate was not ihibited under low-salt conditions. In the dark, CO2-fixation of lysed chloroplasts supplied with ATP, NADPH, and 3-phosphoglycerate strictly required the presence of Mg2+ but was independent of monovalent cations. This finding excludes a direct inactivation of Calvin cycle enzymes as a possible basis for the inhibition of photosynthesis under low-salt conditions. Light-induced alkalization of the stroma and an increase in the concentration of freely exchangeable Mg2+ in the stroma, which can be observed in normal chloroplasts, did not occur under low-salt conditions but were strongly enhanced after addition of monovalent cations (50–100 mM) or Mg2+ (20–50 mM). The relevance of a light-triggered K+/H+ exchange at the chloroplast envelope is discussed with regard to the light-induced increase in the pH and the Mg2+ concentration in the stroma, which are thought to be obligatory for light activation of Calvincycle enzymes.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00380879
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  • 4
    Electronic Resource
    Electronic Resource
    Photosynthesis under osmotic stress (1981)
    Springer
    Planta 153 (1981), S. 430-435 
    Details
    ISSN: 1432-2048
    Keywords: Chloroplast ; Photosynthesis (stress recovery) ; Protoplast ; Spinacia ; Water stress
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The reversibility of the inhibition of photosynthetic reactions by water stress was examined with four systems of increasing complexity—stromal enzymes, intact chloroplasts, mesophyll protoplasts, and leaf slices. The inhibition of soluble chloroplast enzymes by high solute concentrations was instantly relieved when solutes were properly diluted. In contrast, photosynthesis was not restored but actually more inhibited when isolated chloroplasts exposed to hypertonic stress were transferred to conditions optimal for photosynthesis of unstressed chloroplasts. Upon transfer, chloroplast volumes increased beyond the volumes of unstressed chloroplasts, and partial envelope rupture occurred. In protoplasts and leaf slices, considerable and rapid, but incomplete restoration of photosynthesis was observed during transfer from hypertonic to isotonic conditions. Chloroplast envelopes did not rupture in situ during water uptake. It is concluded that inhibition of photosynthesis by severe water stress is at the biochemical level brought about in part by reversible inhibition of chloroplast enzymes and in part by membrane damage which requires repair mechanisms for reversibility. Both soluble enzymes and membranes appear to be affected by the increased concentration of internal solutes, which is caused by dehydration.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00394981
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  • 5
    Electronic Resource
    Electronic Resource
    Increased nitrate reductase activity in leaf tissue after application of the fungicide Kresoxim-methyl (1999)
    Springer
    Planta 207 (1999), S. 442-448 
    Details
    ISSN: 1432-2048
    Keywords: Key words: Nitrate reductase ; Respiration inhibitors ; Spinacia ; Strobilurin
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract. Spinach (Spinacia oleracea L.) leaf discs floating on buffer solution were treated with Kresoxim-methyl (KROM), an inhibitor of respiratory electron transport. In the leaf tissue, actual and maximal nitrate reductase (NR) activities, nitrite content and ATP levels were determined. In darkened leaf discs incubated without KROM (control) actual NR activity decreased to 20% after 6 h in the dark. Treatment with 10 μg ml−1 (corresponding to 32 μM) KROM totally prevented inactivation of NR in the dark and also diminished NR-protein degradation during prolonged darkness. Due to restricted nitrite reduction in darkened leaf tissues, nitrite accumulated in KROM-treated discs. Inhibition of respiration decreased ATP and increased AMP levels in KROM-treated discs. In illuminated leaf discs, NR was highly activated to 65%. Nevertheless, KROM-treatment caused an additional activation of NR (activation state 76%) in the light. Possible side-effects of KROM on nitrite reduction and photosynthesis were also checked in the leaf-disc system. Neither nitrite reduction nor photosynthesis were altered in KROM-treated discs. The extent of KROM-induced activation of NR was dependent on the applied concentration and on the pH of the external medium. The highest activation of NR was achieved at an external pH of 4.8, confirming previous results (Kaiser and Brendle-Behnisch, 1995, Planta 196: 1–6) that cytosolic acidification might play an important role in the modulation of NR activity.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s004250050503
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  • 6
    Electronic Resource
    Electronic Resource
    Acid-base-modulation of nitrate reductase in leaf tissues (1995)
    Springer
    Planta 196 (1995), S. 1-6 
    Details
    ISSN: 1432-2048
    Keywords: Acid-base loading ; Nitrate reductase ; pH regulation (intracellular) ; Protein phosphorylation ; Spinacia
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The effect of acid or base-loading of spinach (Spinacia oleracea L.) leaf discs on the activation status of nitrate reductase (NR) in the dark and in the light was investigated. Activity of NR (NRA), measured in crude extracts of leaf discs with removed lower epidermis, which had been floating on Mes-buffer [2-(N-morpholino)ethane sulfonic acid] pH 5.2 in the dark, was at a similar low level as in whole, darkened leaves. By addition of acetate or propionic acid, butyric acid or benzoic acid, NR was activated to or beyond the light level. The pH of crude tissue extracts was decreased by 0.5–1 pH units. Tissue acidification caused an inhibition of photosynthesis and of dark CO2 fixation. The acid-induced activation of NR in vivo was largely prevented by okadaic acid, an inhibitor of Type 1 and Type 2A protein phosphatases. This indicates that acid-induced activation was mediated by protein dephosphorylation. When, on the other hand, leaf discs were illuminated on Ches-buffer (2-[ N-cyclohexylamino]ethane sulfonic acid) pH 9 in the presence of bicarbonate (80 mM), their NR was as active as in intact leaves. Addition of ammonium chloride (up to 6 mM) caused a pH increase of the tissue extract up to 0.9 pH units. At the same time NR was inactivated to the dark level. Methionine sulfoximine did not prevent the ammonium effect. Photosynthesis and dark CO2 fixation were stimulated at pH 9 by ammonium chloride (1–2· mol· m −3) and were only slightly inhibited by up to 6 mol· m−3. The modulation of NR by acid-base treatment in vivo was fully reversible. The response of the NR system to acid or base treatment is consistent with a proposed role of nitrate reduction in the cellular pH-stat. The observation also indicates that cytosolic pH changes may be involved the signal chain triggering the modulation of NR.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00193210
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  • 7
    Electronic Resource
    Electronic Resource
    Photosynthesis of isolated chloroplasts and protoplasts under osmotic stress (1981)
    Springer
    Planta 151 (1981), S. 375-380 
    Details
    ISSN: 1432-2048
    Keywords: Chloroplast volume ; Photosynthesis and chloroplast volume ; Spinacia ; Water stress
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract 1. Isolated intact spinach chloroplasts respond to changes of the sorbitol concentration of the suspending medium as near-perfect osmometers within a large range of osmotic potentials. Under isotonic conditions (π=9–10 bar), their average osmotic volume is 24 μm3 and the total volume 36 μm3. The osmotic volume can be increased to 63 μm3 by lowering the sorbitol concentration until a critical osmotic potential of π=4 bar is reached. Below that value chloroplasts rupture. Between 10 bar and 4 bar, volume changes are reversible. 2. Increasing the chloroplast volume above 24 μm3 causes inhibition of photosynthesis, with 50% inhibition occurring at an osmotic potential of π=5–6 bar. This corresponds to an osmotic volume of 45–55 μm3. Depending on the duration of hypotonic treatment, inhibition of photosynthesis is more or less reversible. 3. Between 4 and 10 bar, the chloroplast envelope exhibits a very low permeability for ferricyanide, many metabolites, and soluble stroma proteins. 4. Electron transport is not inhibited by swelling of chloroplasts. Also, the ATP/ADP-ratio remains unchanged. 5. The solute concentration in the chloroplasts appears to be optimal for photosynthesis at 10 bar. Increasing the chloroplast volume causes inhibition of photosynthesis by dilution effects.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00393294
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  • 8
    Electronic Resource
    Electronic Resource
    Zytogenetik als Ergänzung zur Histopathologie am Beispiel des myelodysplastischen Syndroms (1994)
    Springer
    Der Pathologe 15 (1994), S. 286-291 
    Details
    ISSN: 1432-1963
    Keywords: Schlüsselwörter Myelodysplastisches Syndrom ; Knochenmark ; Zytogenetik ; Histopathologie ; Prognose ; Key words Myelodysplastic syndrome ; Bone marrow ; Cytogenetics ; Histopathology ; Prognosis
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Description / Table of Contents: Summary The value of cytogenetics performed simultaneously with histopathology was evaluated in patients with myelodysplastic syndrome (MDS). Clonal karyotype changes of the bone marrow cells supporting the histological diagnosis were found in 38/69 cases (55 %). The chromosome aberrations, especially complex changes, were significantly correlated to distinct histopathological findings such as atypias of the haematopoietic cell lines and myelosclerosis. Complex karyotype changes were further associated with short survival of the MDS patients. Our results demonstrate that cytogenetic analyses are helpful in supplementing the histopathological diagnoses. Recent developments in molecular cytogenetics even allow the detection of chromosomal aberrations in non-dividing cells from cytological preparations or tissue sections which may become available for routine diagnosis.
    Notes: Zusammenfassung Die Bedeutung simultaner zytogenetischer und histologischer Untersuchungen wurde bei Patienten mit myelodysplastischem Syndrom (MDS) überprüft. Die Ergebnisse zeigen, daß klonale Karyotypveränderungen der Knochenmarkzellen bei 38 der 69 (55 %) analysierten Patienten auftraten und damit häufig eine Absicherung der histologischen Diagnose erlaubten. Die Chromosomenanomalien, insbesondere komplexe Karyotypveränderungen, korrelierten signifikant mit einer Reihe histopathologischer Befunde, darunter Atypien der einzelnen hämatologischen Zellreihen und Myelosklerose. Durch den Nachweis komplexer Karyotypveränderungen war eine unabhängige prognostische Aussage möglich. Damit zeigen unsere Ergebnisse am Beispiel des MDS, daß zytogenetische Analysen eine sinnvolle Ergänzung der histologischen Untersuchung sein können. Darüber hinaus ist durch den Einsatz der molekularen Zytogenetik die Bestimmung von Chromosomenanomalien in zytologischen Ausstrichpräparaten oder Gewebeschnitten möglich, wodurch sich solche Befunde auch für die tägliche Diagnostik verwenden lassen.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s002920050056
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  • 9
    Electronic Resource
    Electronic Resource
    Vergleichende DNA-zytometrische und zytogenetische Ploidie- bestimmungen an Chondro- sarkomen und Osteosarkomen (1996)
    Springer
    Der Pathologe 17 (1996), S. 374-379 
    Details
    ISSN: 1432-1963
    Keywords: Schlüsselwörter Knochentumoren ; Chondrosarkom ; Osteosarkom ; Zytogenetik ; DNA-Zytometrie ; Ploidie ; Key words Bone neoplasms ; Chondrosarcoma ; Osteosarcoma ; Cytogenetics ; DNA-cytometry ; Ploidy
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Description / Table of Contents: Summary 10 chondrosarcomas and 10 osteosarcomas were examined using cytogenetics and DNA-image-cytometry. Cytogenetically 6 of 10 chondrosarcomas and 4 of 10 osteosarcomas showed hyperdiploid tumorcells. By DNA-cytometry in 8 of 10 chondrosarcomas and 9 of 10 osteosarcomas hyperdiploid tumorcells resp. hyperdiploid stemlines were detected. This discrepancy reflects an in-vitro-selection depending on the different entities. In 7 aneuploid clones of chondrosarcomas the chromosomal ploidy was calculated using the relative length of the chromosomes and compared with the DNA-ploidy of the native tumor. There was a close relation between both parameters of nuclear DNA-content. The interpretation of cytogenetic results is improved using a combination of karyotypic and DNA-cytometric examination. This is particularly important for the search for relations between numeric chromosomal aberrations and morphological parameters (grading).
    Notes: Zusammenfassung 10 Chondrosarkome und 10 Osteosarkome wurden tumorzytogenetisch und DNA-zytometrisch untersucht. Das Karyogramm erbrachte bei 6 von 10 Chondrosarkomen und bei 4 von 10 Osteosarkomen den Nachweis hyperdiploider Tumorzellklone. DNA-zytometrisch wurden am nativen Tumormaterial jedoch bei 8 von 10 Chondrosarkomen und bei 9 von 10 Osteosarkomen hyperdiploide Tumorzellen, häufig in Form eigenständiger hyperdiploider Stammlinien nachgewiesen. Diese Diskrepanz ist Ausdruck einer offenbar Entitäts-abhängigen In-vitro-Selektion. Bei insgesamt 7 aneuploiden Tumorzellklonen von Chondrosarkomen konnte die chromosomale Ploidie anhand der relativen Chromosomenlängen exakt errechnet und der zytometrisch bestimmten DNA-Ploidie gegenübergestellt werden, wobei sich eine sehr enge Abhängigkeit zwischen diesen beiden Parametern des nukleären DNA-Gehaltes ergab. Die Interpretation zytogenetischer Befunde bei Knochentumoren wird durch Kombination mit der DNA-Zytometrie verbessert. Dies ist besonders dann wichtig, wenn Zusammenhänge zwischen numerischen chromosomalen Aberrationen und morphologischen Parametern (z. B. Grading) dargestellt werden sollen.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s002920050175
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  • 10
    Electronic Resource
    Electronic Resource
    Zytogenetik und molekulare Untersuchungen sichern die histopathologischen Diagnosen von chronischen myelopro-liferativen Erkrankungen ab (1995)
    Springer
    Der Pathologe 16 (1995), S. 41-45 
    Details
    ISSN: 1432-1963
    Keywords: Schlüsselwörter Chronische myeloproliferative Erkrankungen ; Philadelphia-Translokation ; Zytogenetik ; Molekulargenetik ; Fluoreszenz-in-situ-Hybridisierung ; Histopathologie ; Key words Chronic myeloproliferative disorders ; Philadelphia-translocation ; Cytogenetics ; Molecular genetics ; Fluorescence in situ hybridization ; Histopathology
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Description / Table of Contents: Summary The histopathological classification of chronic myeloproliferative disorders can be supported by applying cytogenetics and molecular genetics to the analysis of bone marrow or blood cells, as demonstrated in 253 cases evaluated. The Philadelphia translocation (9;22) is the most important genetic parameter, being specific for chronic myeloid leukemia. Conventional methods for the detection of the t(9;22) are karyotyping and Southern blot analysis of the bcr gene. The newly established technique of fluorescence in situ hybridization (FISH) allows visualization of bcr-abl fusion even in non dividing cells. Molecular cytogenetics for t(9;22) yield results that are rapid and reliable as well as easily quantifiable.
    Notes: Zusammenfassung Zytogenetische und molekulargenetische Untersuchungen von Knochenmark- oder Blutzellen sind für die histopathologische Klassifikation der chronischen myeloproliferativen Erkrankungen hilfreich, was durch die simultane Auswertung von 253 Fällen gezeigt wird. Insbesondere die Analyse der Philadelphia-Translokation (9;22) ist dabei für die Bestätigung oder den Ausschluß einer chronischen myeloischen Leukämie wichtig. Für den Nachweis der t(9;22) stehen die konventionelle Karyotypisierung mit Bestimmung des Philadelphia-Chromosoms und das Southernblotverfahren zur Analyse einer Umlagerung des bcr-Gens zur Verfügung. Durch die neuere Methode der Fluoreszenz-in-situ-Hybridisierung (FISH) kann auch eine bcr-abl-Fusion an Interphasekernen dargestellt werden. Diese molekulare Zytogenetik ist ein rasches und zuverlässiges Verfahren zum Nachweis der Philadelphia-Translokation, das zudem leicht quantifizierbare Ergebnisse liefert.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s002920050074
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