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  • Nicotiana tabacum  (5)
  • Cytoskeleton  (4)
  • Cytokinesis  (2)
  • In vitro germination  (2)
  • 1
    Electronic Resource
    Electronic Resource
    Distribution of microtubules during the growth of tobacco pollen tubes
    Amsterdam : Elsevier
    Biology of the Cell 79 (1993), S. 125-132 
    Details
    ISSN: 0248-4900
    Keywords: Nicotiana tabacum ; acetylated α-tubulin ; confocal laser scanning microscope ; microtubules ; pollen tube growth ; tyrosinated α-tubulin
    Source: Elsevier Journal Backfiles on ScienceDirect 1907 - 2002
    Topics: Biology
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1016/0248-4900(93)90248-D
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  • 2
    Electronic Resource
    Electronic Resource
    Cytokinesis in microspore mother cells of Impatiens sultani (1988)
    Springer
    Sexual plant reproduction 1 (1988), S. 228-233 
    Details
    ISSN: 1432-2145
    Keywords: Microspore mother cell ; Cytokinesis ; Impatiens sultani ; Cell plate
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Cytokinesis in Impatiens sultani microspore mother cells is simultaneous. It starts with the formation of small ingrowths of the surrounding callosic wall. Next, an incomplete cell plate is formed by fusion of small dictyosome vesicles. The cell plate consists of a network of anastomosing tubules and sacs. Aggregates of fusing vesicles are associated with bundles of microtubules, which are oriented perpendicular to the plane of the future cell walls. In the sacculate parts of the cell plate, some callose is deposited, while the associated microtubules disappear. The cell walls ultimately develop by enlargement of the previously formed wall ingrowths, which successively incorporate the elements of the cell plate. The enlargement and thickening of the walls is not accompanied by a further fusion and incorporation of dictyosome vesicles.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00189157
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  • 3
    Electronic Resource
    Electronic Resource
    In vitro double fertilization in Nicotiana tabacum (L.): polygamy compared with selected single pair somatic protoplast and chloroplast fusions (2000)
    Springer
    Sexual plant reproduction 13 (2000), S. 113-117 
    Details
    ISSN: 1432-2145
    Keywords: Key words Cell fusion ; Gamete interaction ; In vitro polygamy ; Nicotiana tabacum
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract  In vitro polygamy was studied mainly by using isolated sperm and central cells of tobacco in order to elucidate the mechanism that might be involved in preventing in vivo polygamy. In 17.5% 4000 M.W. polyethylene glycol, only when two sperm cells were made close enough to each other and adhered to a female cell simultaneously was polygamy possible. If one sperm cell fused with the egg or central cell, within 30 min another sperm cell could not fuse with the same egg or central cell. Similar phenomena were found in selected single somatic cell fusion. When more than two protoplasts adhered to each other simultaneously, fusion was always successful; after two protoplasts fused, within 30 min the fusion products could not fuse with another protoplast under the same conditions. This comparative study revealed this characteristic to be shared by both sexual and somatic cell fusion. However, after cytoplasm reorganization was complete in the fusion product, it was possible for the fusion product to fuse with the third protoplast. This indicates that the obstruction to additional fusion was present only during a certain period after the preceding fusion under certain condition. The possible reason for the effect is discussed.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s004970000044
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  • 4
    Electronic Resource
    Electronic Resource
    Responses of tobacco pollen to high humidity and heat stress: viability and germinability in vitro and in vivo (1991)
    Springer
    Sexual plant reproduction 4 (1991), S. 104-109 
    Details
    ISSN: 1432-2145
    Keywords: High humidity and temperature stress ; Nicotiana tabacum ; Tobacco ; Pollen viability ; Vigour ; Semi-vivo technique
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Responses of pollen grains of Nicotiana tabacum to high humidity (95% RH, 4 h) and temperature (38°/45° C, 4 h) stresses were investigated. Pollen grains were subjected to only RH or only temperature, or to both of these stresses. Their viability was assessed on the basis of the fluorochromatic reaction (FCR) test, and vigour was assessed on the basis of the time taken for in vitro germination as well as on the emergence of pollen tubes through the cut end of semi-vivo implanted styles. None of the stress conditions affected pollen viability and high RH or high temperature stress did not individually affect pollen vigour. However, pollen vigour was markedly affected when both the stresses were given together. Pollen grains subjected to high RH at 38° C took a longer time to germinate in vitro and the pollen tubes emerged later from the cut end of the semi-vivo styles; division of the generative cell was also delayed. Pollen grains subjected to high RH at 45° C failed to germinate in vitro, but did germinate on the stigma. Many pollen tubes subjected to this treatment showed abnormalities, and the growth of pollen tubes in the pistil was much slower than that observed in other treatments. Pollen samples subjected to all of the stress conditions were able to induce fruit and seed set. The implications of these results on the relationship between the FCR test and viability, and between viability and vigour, especially in stressed pollen, are discussed.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00196495
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  • 5
    Electronic Resource
    Electronic Resource
    Germination and early tube development in vitro of Lycopersicum peruvianum pollen: Ultrastructural features (1977)
    Springer
    Planta 136 (1977), S. 239-247 
    Details
    ISSN: 1432-2048
    Keywords: Early tube development ; In vitro germination ; Lycopersicum ; Pollen
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Morphologic changes occurring during pollen grain activation and ultrastructural features of Lycopersicum peruvianum Mill. pollen tube during the first stages of growth in vitro have been studied. The more evident morphologic changes during activation, in comparison to those already described for mature inactive pollen, concern dictyosomes, rough endoplasmic reticulum (RER), and ribosomes. The dictyosomes are very abundant and produce “large” and “small” vesicles. Near the germinative pores both types of vesicles are present, while all along the remaining cell wall only the large type is observed. These latter react weakly to Thiéry's test and probably contain a callose precursor necessary for the deposition of a callosic layer lining at first only the inner side of the functioning pore and occasionally the other two pores, and subsequently the entire inner surface of the cell wall. The small vesicles, highly positive to Thiéry's test, are present only near the pores and could be involved in the formation of the pectocellulosic layer of the tube wall. The setting free of RER cisterns, which in the mature inactive pollen were aggregated in stacks, coinciding with polysome formation and resumption of protein synthesis, is in accord with the hypothesized role of RER cistern stacks as a reserve of synthesizing machinery. The pollen tube reaches a definitive spatial arrangement soon after the generative cell and vegetative nucleus have moved into it. At this stage four different zones that reflect a functional specialization are present. In the apical and subapical zone two types of dictysosome-originated vesicles, similar to those found in the activated pollen grain, are present. Their role in the formation of the callosic and pectocellulosic wall layers seems to be the same as in the activated pollen grain.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00385991
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  • 6
    Electronic Resource
    Electronic Resource
    Pollen viability and pollen vigor (1991)
    Springer
    Theoretical and applied genetics 81 (1991), S. 38-42 
    Details
    ISSN: 1432-2242
    Keywords: High temperature and humidity stress ; In vitro germination ; Pollen grains ; Storage ; Viability and vigor
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Investigations were carried out to correlate pollen viability, assessed on the basis of a fluorochromatic reaction (FCR) test, with pollen vigor, assessed on the basis of the time taken for in vitro germination in pollen grains subjected to high humidity (〉95% RH) and temperature (38 °C) or storage stress of Nicotiana tabacum, Agave sp., Tradescantia virginiana, and Iris sp. Both high RH and temperature, as well as storage stresses, affected pollen vigor before affecting pollen viability. The results are discussed in the light of available data on the viability and vigor of stressed pollen and of aged seeds. The need for consideration of pollen vigor, particularly in stored pollen, the inadequacy of the methods presently used, and some of the methods suitable to assess pollen vigor are elaborated.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00226109
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  • 7
    Electronic Resource
    Electronic Resource
    Ultrastructural features of Aloe ciliaris pollen (1988)
    Springer
    Sexual plant reproduction 1 (1988), S. 88-96 
    Details
    ISSN: 1432-2145
    Keywords: Pollen activation ; Generative cell ; Vegetative cell ; Cytoskeleton
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Both the internal anatomy and the external morphology of the mature pollen grain of Aloe ciliaris have been studied, together with the cytological changes occurring during pollen activation. In mature pollen, the generative cell (GC) and the vegetative nucleus (VN) are closely associated with each other, and both can be found in the central part of the grain. In the generative cytoplasm, some organelles and microtubular bundles are present. In the vegetative cell, dictyosomes, stacks of rough endoplasmic reticulum, mitochondria, plastids, vacuoles, ribosomes, and masses of fibrillar material have been described. During pollen activation, important changes occur in both the generative and vegetative cells (VC). In the GC, the microtubular bundles become clearly visible, and the GC and VC gradually move towards the germ pore. The RER cisterns become free from the stacks, and organelles, such as dictyosomes, become very active. The fibrillar masses gradually decrease in number, and the individual fibrils become more evident and clearer in resolution.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00189267
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  • 8
    Electronic Resource
    Electronic Resource
    Effects of high humidity and temperature stress on pollen membrane integrity and pollen vigour in Nicotiana tabacum (1989)
    Springer
    Sexual plant reproduction 2 (1989), S. 137-141 
    Details
    ISSN: 1432-2145
    Keywords: Humidity ; Temperature stress ; Nicotiana tabacum ; Pollen germination ; Pollen membrane
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary The prolonged exposure of pollen Nicotiana tabacum to high humidity at both room temperature and 38° C did not affect membrane integrity as revealed by the fluorochromatic reaction (FCR) test, but did affect pollen vigour. At room temperature germination was not affected, although tube growth was reduced; at 38° C, there was both a reduction in tube growth and delayed germination. When the pollen was subjected to 1 h hydration followed by 1 h desiccation (up to a maximum of four cycles) at room temperature, a reduction in the FCR, germination and tube length after each desiccation treatment was observed. Subsequent hydration fully restored the FCR, but only partially restored germination and tube growth. At 38° C, however, FCR, germination, and tube growth were drastically reduced. The implications of these results on the relationship between FCR and germinability, the responses of pollen exposed to humidity and temperature stress in the field, and on pollen storage are discussed.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00192759
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  • 9
    Electronic Resource
    Electronic Resource
    Striped projections of the outer membrane of the generative cell in Convallaria majalis pollen (1995)
    Springer
    Sexual plant reproduction 8 (1995), S. 223-227 
    Details
    ISSN: 1432-2145
    Keywords: Pollen ; Generative cell ; Cytoskeleton ; Motility ; Myosin immunogold labelling
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract In pollen grains of Convallaria majalis the outer membrane of the generative cell (GC) is the inner membrane of the vegetative cell (VC). Striped projections (SP) at the cytoplasmic face of the outer membrane of the GC were revealed by chemical fixation and also by a rapid freeze-fixation and freeze-substitution. The projections, located in groups on the protruding lobes of the GC, were arranged parallel to each other and were equally spaced (40 nm apart). The length of the SP, estimated from grazing sections of GC, was 400 nm. Each projection was composed of T-shaped elements, about 35 nm high, spaced at an average distance of 25 nm. SP were observed in mature, hydrated, activated and germinated pollen grains and seemed to be associated with microtubules and microfilaments of the VC. No evidence exists yet of SP on the sperm cell membrane. Immunogold labelling with anti-myosin antibodies showed many gold particles attached preferentially to the surface of the protruding lobes of the GC in the area of the projections. These results may suggest that the SP of Convallaria GC contain myosin-like protein and play an important role in the motility of the GC during pollen tube growth.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00228941
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  • 10
    Electronic Resource
    Electronic Resource
    Ultrastructure of the cytoskeleton in freeze-substituted pollen tubes ofNicotiana alata (1987)
    Springer
    Protoplasma 140 (1987), S. 141-150 
    Details
    ISSN: 1615-6102
    Keywords: Cytoskeleton ; Freeze substitution ; Nicotiana ; Pollen tubes ; Rapid freeze fixation
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary The ultrastructure of the cytoskeleton inNicotiana alata pollen tubes grownin vitro has been examined after rapid freeze fixation and freeze substitution (RF-FS). Whereas cytoplasmic microtubules (MTs) and especially microfilaments (MFs) are infrequently observed after conventional chemical fixation, they occur in all samples prepared by RF-FS. Cortical MTs are oriented parallel to the long axis of the pollen tube and usually appear evenly spaced around the circumference of the cell. They are always observed with other components in a structural complex that includes the following: 1. a system of MFs, in which individual elements are aligned along the sides of the MTs and crossbridged to them; 2. a system of cooriented tubular endoplasmic reticulum (ER) lying beneath the MTs, and 3. the plasma membrane (PM) to which the MTs appear to be extensively linked. The cortical cytoskeleton is thus structurally complex, and contains elements such as MFs and ER that must be considered together with the MTs in any attempt to elucidate cytoskeletal function. MTs are also observed within the vegetative cytoplasm either singly or in small groups. Observations reveal that some of these may be closely associated with the envelope of the vegetative nucleus. MTs of the generative cell, in contrast to those of the vegetative cytoplasm, occur tightly clustered in bundles and show extensive cross-bridging. These bundles, especially in the distal tail of the generative cell, are markedly undulated. MFs are observed commonly in the cytoplasm of the vegetative cell. They occur in bundles oriented predominantly parallel to the pollen tube axis. Although proof is not provided, we suggest that they are composed of actin and are responsible for generating the vigorous cytoplasmic streaming characteristic of living pollen tubes.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01273723
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