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  • 1
    Electronic Resource
    Electronic Resource
    In vitro double fertilization in Nicotiana tabacum (L.): polygamy compared with selected single pair somatic protoplast and chloroplast fusions (2000)
    Springer
    Sexual plant reproduction 13 (2000), S. 113-117 
    Details
    ISSN: 1432-2145
    Keywords: Key words Cell fusion ; Gamete interaction ; In vitro polygamy ; Nicotiana tabacum
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract  In vitro polygamy was studied mainly by using isolated sperm and central cells of tobacco in order to elucidate the mechanism that might be involved in preventing in vivo polygamy. In 17.5% 4000 M.W. polyethylene glycol, only when two sperm cells were made close enough to each other and adhered to a female cell simultaneously was polygamy possible. If one sperm cell fused with the egg or central cell, within 30 min another sperm cell could not fuse with the same egg or central cell. Similar phenomena were found in selected single somatic cell fusion. When more than two protoplasts adhered to each other simultaneously, fusion was always successful; after two protoplasts fused, within 30 min the fusion products could not fuse with another protoplast under the same conditions. This comparative study revealed this characteristic to be shared by both sexual and somatic cell fusion. However, after cytoplasm reorganization was complete in the fusion product, it was possible for the fusion product to fuse with the third protoplast. This indicates that the obstruction to additional fusion was present only during a certain period after the preceding fusion under certain condition. The possible reason for the effect is discussed.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s004970000044
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  • 2
    Electronic Resource
    Electronic Resource
    Dynein-related polypeptides in pollen and pollen tubes (1996)
    Springer
    Sexual plant reproduction 9 (1996), S. 312-317 
    Details
    ISSN: 1432-2145
    Keywords: Pollen tube ; Nicotiana tabacum ; Ginkgo biloba ; Dynein
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Microtubules in pollen tubes are evident within the vegetative and generative cell cytoplasm. This observation led to the formulation of several hypotheses regarding the role of microtubules in cytoplasmic movement and the migration of the vegetative nucleus/generative cell along the pollen tube. The study of microtubular motor proteins in pollen tubes followed the discovery and characterization of an immunoreactive homolog of mammalian kinesin in tobacco pollen tubes. Recent identification of dynein-related polypeptides in pollen tubes ofNicotiana tabacum and pollen ofGinkgo biloba is a significant step in the definition of the role of microtubule function within pollen and pollen tubes.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02441948
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    Paper (German National Licenses)
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  • 3
    Electronic Resource
    Electronic Resource
    An immunoreactive homolog of mammalian kinesin in Nicotiana tabacum pollen tubes (1992)
    New York, NY : Wiley-Blackwell
    Cell Motility and the Cytoskeleton 21 (1992), S. 132-137 
    Details
    ISSN: 0886-1544
    Keywords: microtubules ; vesicles ; cytoplasmic movement ; monoclonal antibody ; Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Medicine
    Notes: A cytoskeletal apparatus is involved in the movement of vesicles, organelles, and gametes in the pollen tube. The function of microfilaments has been defined quite precisely, but the role of microtubules needs to be further clarified. On the basis of immunological and biochemical investigations, we have identified a polypep-tide showing common properties with kinesin, a microtubule-based motor mainly described in nonplant tissues, in the pollen tube of Nicotiana tabacum. Like mammalian kinesin, the kinesin-immunoreactive homolog from Nicotiana tabacum pollen tubes binds to mammalian microtubules in an AMP-PNP dependent manner. The kinesin-like component is likely to be involved in the movement of vesicular material in the growing pollen tube.
    Additional Material: 4 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/cm.970210206
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    Paper (German National Licenses)
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