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  • 1
    Electronic Resource
    Electronic Resource
    Dynein heavy chain-related polypeptides are associated with organelles in pollen tubes of Nicotiana tabacum (1998)
    Springer
    Sexual plant reproduction 11 (1998), S. 31-40 
    Details
    ISSN: 1432-2145
    Keywords: Key words Pollen tube ; Nicotiana tabacum ; Dynein heavy chain related polypeptides ; Immunolocalization
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract  It is known that pollen tubes contain two high molecular weight polypeptides which share some biochemical and immunological properties with dynein heavy chains. This paper reports data on the subcellular localization of the two dynein heavy chain-related polypeptides during pollen tube growth. Immunofluoresence studies using a purified antibody (Dy-1) raised against a synthetic peptide reproducting the P-loop conserved sequence of dynein heavy chains showed spot-like structures, with a characteristic distribution pattern that depended on the tube length. Biochemical evidence confirmed the presence of dynein heavy chain-related bands in the pollen tube membrane fraction. The association of proteins carrying dynein heavy chain-related polypeptides to cell membranes was affected by detergent (Triton×100), whereas other stripping agents, like NaCl and Na2CO3, did not significantly influence the interaction of dynein heavy chain-related doublet with their cytoplasmic targets. These data suggest that dynein heavy chain-related polypeptides associate with membranous organelles within the vegetative cell of Nicotiana tabacum pollen tubes, implying their involvement in the cytoplasmic distribution of these organelles.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s004970050118
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    Paper (German National Licenses)
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  • 2
    Electronic Resource
    Electronic Resource
    Dynein-related polypeptides in pollen and pollen tubes (1996)
    Springer
    Sexual plant reproduction 9 (1996), S. 312-317 
    Details
    ISSN: 1432-2145
    Keywords: Key words Pollen tube ; Nicotiana tabacum ; Ginkgo biloba ; Dynein
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract  Microtubules in pollen tubes are evident within the vegetative and generative cell cytoplasm. This observation led to the formulation of several hypotheses regarding the role of microtubules in cytoplasmic movement and the migration of the vegetative nucleus/generative cell along the pollen tube. The study of microtubular motor proteins in pollen tubes followed the discovery and characterization of an immunoreactive homolog of mammalian kinesin in tobacco pollen tubes. Recent identification of dynein-related polypeptides in pollen tubes of Nicotiana tabacum and pollen of Ginkgo biloba is a significant step in the definition of the role of microtubule function within pollen and pollen tubes.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s004970050048
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    Paper (German National Licenses)
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  • 3
    Electronic Resource
    Electronic Resource
    Dynein-related polypeptides in pollen and pollen tubes (1996)
    Springer
    Sexual plant reproduction 9 (1996), S. 312-317 
    Details
    ISSN: 1432-2145
    Keywords: Pollen tube ; Nicotiana tabacum ; Ginkgo biloba ; Dynein
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Microtubules in pollen tubes are evident within the vegetative and generative cell cytoplasm. This observation led to the formulation of several hypotheses regarding the role of microtubules in cytoplasmic movement and the migration of the vegetative nucleus/generative cell along the pollen tube. The study of microtubular motor proteins in pollen tubes followed the discovery and characterization of an immunoreactive homolog of mammalian kinesin in tobacco pollen tubes. Recent identification of dynein-related polypeptides in pollen tubes ofNicotiana tabacum and pollen ofGinkgo biloba is a significant step in the definition of the role of microtubule function within pollen and pollen tubes.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02441948
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    Paper (German National Licenses)
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  • 4
    Electronic Resource
    Electronic Resource
    An immunoreactive homolog of mammalian kinesin in Nicotiana tabacum pollen tubes (1992)
    New York, NY : Wiley-Blackwell
    Cell Motility and the Cytoskeleton 21 (1992), S. 132-137 
    Details
    ISSN: 0886-1544
    Keywords: microtubules ; vesicles ; cytoplasmic movement ; monoclonal antibody ; Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Medicine
    Notes: A cytoskeletal apparatus is involved in the movement of vesicles, organelles, and gametes in the pollen tube. The function of microfilaments has been defined quite precisely, but the role of microtubules needs to be further clarified. On the basis of immunological and biochemical investigations, we have identified a polypep-tide showing common properties with kinesin, a microtubule-based motor mainly described in nonplant tissues, in the pollen tube of Nicotiana tabacum. Like mammalian kinesin, the kinesin-immunoreactive homolog from Nicotiana tabacum pollen tubes binds to mammalian microtubules in an AMP-PNP dependent manner. The kinesin-like component is likely to be involved in the movement of vesicular material in the growing pollen tube.
    Additional Material: 4 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/cm.970210206
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    Paper (German National Licenses)
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  • 5
    Electronic Resource
    Electronic Resource
    Kinesin-related polypeptide is associated with vesicles from Corylus avellana pollen (1994)
    New York, NY : Wiley-Blackwell
    Cell Motility and the Cytoskeleton 29 (1994), S. 155-166 
    Details
    ISSN: 0886-1544
    Keywords: Golgi vesicles ; pollen ; pollen tube ; microtubules ; kinesin-related protein ; Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Medicine
    Notes: A 100-kDa polypeptide with microtubule-interacting properties was identified in a Golgi vesicle-enriched fraction from Corylus avellana pollen. The k71s23 antibody (directed to the kinesin heavy chain from bovine brain) [Tiezzi et al., 1992: Cell Motil. Cytoskeleton 21:132-137] localized the polypeptide on the external surface of membrane-bounded organelles. Some 100-kDa-containing vesicles co-pelleted with microtubules (polymerized from purified bovine brain tubulin) either in presence or absence of 5 mM AMPPNP, but they could be released by 10 mM ATP or 0.5 M KCl. The pollen microtubule-interacting protein, salt-extracted from membranes and partially purified by gel filtration, exhibited an ATPase activity (16.2 nmolPi/mg/min) which could be stimulated about 2-fold (32.5 nmolPi/mg/min) by addition of bovine brain microtubules. We suppose that the 100-kDa polypeptide is part of a molecular complex showing properties of the kinesin class. © 1994 Wiley-Liss, Inc.
    Additional Material: 9 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/cm.970290207
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    Paper (German National Licenses)
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