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  • 1
    Electronic Resource
    Electronic Resource
    Dynein heavy chain-related polypeptides are associated with organelles in pollen tubes of Nicotiana tabacum (1998)
    Springer
    Sexual plant reproduction 11 (1998), S. 31-40 
    Details
    ISSN: 1432-2145
    Keywords: Key words Pollen tube ; Nicotiana tabacum ; Dynein heavy chain related polypeptides ; Immunolocalization
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract  It is known that pollen tubes contain two high molecular weight polypeptides which share some biochemical and immunological properties with dynein heavy chains. This paper reports data on the subcellular localization of the two dynein heavy chain-related polypeptides during pollen tube growth. Immunofluoresence studies using a purified antibody (Dy-1) raised against a synthetic peptide reproducting the P-loop conserved sequence of dynein heavy chains showed spot-like structures, with a characteristic distribution pattern that depended on the tube length. Biochemical evidence confirmed the presence of dynein heavy chain-related bands in the pollen tube membrane fraction. The association of proteins carrying dynein heavy chain-related polypeptides to cell membranes was affected by detergent (Triton×100), whereas other stripping agents, like NaCl and Na2CO3, did not significantly influence the interaction of dynein heavy chain-related doublet with their cytoplasmic targets. These data suggest that dynein heavy chain-related polypeptides associate with membranous organelles within the vegetative cell of Nicotiana tabacum pollen tubes, implying their involvement in the cytoplasmic distribution of these organelles.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s004970050118
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  • 2
    Electronic Resource
    Electronic Resource
    Generative cell division and sperm cell formation in barley (1988)
    Springer
    Sexual plant reproduction 1 (1988), S. 240-247 
    Details
    ISSN: 1432-2145
    Keywords: Cell division ; Pollen ; Generative cell ; Sperm cells ; Hordeum vulgare L.
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Shortly before and during division, the generative cell of barley (Hordeum vulgare L.) is located near the vegetative nucleus, in the peripheral layer of the highly vacuolated vegetative cell at the aperture pole. This position is also characteristic of the two resulting sperm cells. Conventional mitosis of the generative cell is followed by cytokinesis through cell plate formation. Just after division, the two sperm cells are enclosed together within a common “inner” vegetative cell plasma membrane, and they gradually separate from each other only during pollen maturation. The space between the generative or sperm cell plasma membrane and the vegetative cell plasma membrane is very thin and appears to be devoid of a cell wall. Both the generative cell and the young sperm cells contain a normal set of organelles; plastids devoid of starch are only sporadically observed. Our data indicate that in Hordeum vulgare the generative cell divides after migrating inside the pollen grain. This follows the pattern of development well established for several species with tricellular pollen.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00189159
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    Paper (German National Licenses)
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  • 3
    Electronic Resource
    Electronic Resource
    Ultrastructure of the stylar canal cells ofCitrus limon (Rutaceae) (1981)
    Springer
    Plant systematics and evolution 138 (1981), S. 263-274 
    Details
    ISSN: 1615-6110
    Keywords: Angiosperms ; Rutaceae ; Citrus limon ; Style ; canal cells ; canal filling substance ; ultrastructure ; anatomy
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The ultrastructure of the canal cells and the canal filling substance ofCitrus limon have been studied. At maturity the canal cells are very rich in cytoplasm. Their inner tangential walls lining the canal are much thickened and formed by two layers: the outer corresponds to the original wall, the inner is formed by subsequent deposition of abundant materials of different origin. This thickening occurs at the same time as the filling of the stylar canal. Both events are paralleled by considerable dictyosomic activity, the formation of a large amount of rough endoplasmic reticulum, and the incorporation of small cytoplasmic masses into the cell wall, due to plasmalemma evaginations. — The material in the stylar canal has a heterogeneous ultrastructure aspect and consists of polysaccharides, proteins and lipids; it presumably provides nutrients for the growing pollen tubes.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00985190
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    Paper (German National Licenses)
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