ISSN:
1432-2048
Schlagwort(e):
Cell culture
;
Cytokinin and embryogenesis
;
Embryonic/proembryonic development
;
Pimpinella
Quelle:
Springer Online Journal Archives 1860-2000
Thema:
Biologie
Notizen:
Abstract Endogenous levels of cytokinins in an anise cell culture were determined by the use of radioimmunoassay and gas chromatography-mass spectrometry in combination with single-ion monitoring, during proembryonal and embryonal development. In both cultures the highest cytokinin levels were correlated with logarithmic growth (embryonal: isopentenyladenosine, 4 ng g-1 fresh weight [FW]; isopentenyladenine, 1.4 ng g-1 FW; zeatin, 3.6 ng g-1 FW; proembryonal: isopentenyladenosine, 58.3 ng g-1 FW; isopentenyladenine, 7.9 ng g-1 FW; zeatin 11.1 ng g-1 FW). The proembryonic culture medium but not the embryonic culture medium contained isopentenyladenosine up to 28 pg ml-1 during logarithmic growth. No correlation between different embryonic stages and the endogenous cytokinin level was obvious.
Materialart:
Digitale Medien
URL:
http://dx.doi.org/10.1007/BF00982919
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