ISSN:
1432-2242
Keywords:
Hordeum vulgare ssp
;
spontaneum Hordeum vulgare ssp
;
vulgare
;
Genetic distance
;
Fixation index
;
Restriction fragment length polymorphism
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
Notes:
Abstract Genetic variability of cultivated and wild barley, Hordeum vulgare ssp. vulgare and spontaneum, respectively, was assessed by RFLP analysis. The material consisted of 13 European varietes, single-plant offspring lines of eight land races from Ethiopia and Nepal, and five accessions of ssp. spontaneum from Israel, Iran and Turkey. Seventeen out of twenty-one studied cDNA and gDNA probes distributed across all seven barley chromosomes revealed polymorphism when DNA was digested with one of four restriction enzymes. A tree based on genetic distances using frequencies of RFLP banding patterns was estimated and the barley lines clustered into five groups reflecting geographical origin. The geographical groups of land-race lines showed less intragroup variation than the geographical groups of spontaneum lines. The group of European varieties, representing large variation in agronomic traits, showed an intermediate level. The proportion of gene diversity residing among geographical groups (FST) varied from 0.19 to 0.94 (average 0.54) per RFLP pattern, indicating large diversification between geographical groups.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF00223704
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