ISSN:
1434-0879
Keywords:
Nuclear protein
;
DNA
;
Prostatic adenocarcinoma
;
Cell proliferation
Source:
Springer Online Journal Archives 1860-2000
Topics:
Medicine
Notes:
Abstract The value of nuclear protein (NP) as a prognostic parameter in prostatic adenocarcinoma was investigated. The NP and DNA contents of two prostatic tumour lines with a well-documented hormonal dependency (PC-82, PC-EW) were compared to the NP and DNA contents of two xenografts with only partial or no response to androgen deprivation (LNCaP, PC-133). After hormonal treatment the PC-82 and PC-EW tumours showed a significant decrease in the NP/DNA ratio, which coincided with a decrease in the proliferative activity [anti-bromodeoxyuridine 9BRDU] antibody-labelling index] of the same specimens. In the fast-growing LNCaP tumour an increased percentage of cells with high NP and DNA contents was found. The tumours PC-82, PC-EW, and PC-133 with lower proliferative activity showed lower nuclear protein and DNA contents. In a pilot study of 20 prostatic biopsies the amount of nuclear protein and DNA in grade 1–3 tumours as well as in dysplasia was measured. Statistically significant differences (P〈0.002) were found between grade 1 and grade 3 tumours. The mean NP/DNA ratio was increased in high-grade malignancies. Nuclear protein appeared to be a potential parameter in predicting growth activity in prostatic carcinoma.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF00304773
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