Library

X
feed icon rss

Your email was sent successfully. Check your inbox.

An error occurred while sending the email. Please try again.

Proceed reservation?

Export
  • 1
    Electronic Resource
    Electronic Resource
    Flow cytometric analysis of DNA content in bladder cancer: prognostic value of the DNA-index with respect to early tumour recurrence in G2 tumours (1986)
    Springer
    World journal of urology 4 (1986), S. 205-210 
    Details
    ISSN: 1433-8726
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary Flow cytometric DNA analysis was performed on 167 biopsies from 131 patients with transitional cell carcinoma of the bladder, all histologically confirmed. The degree of aneuploidy increased with tumour grade: G1 tumours were generally diploid (71%); G2, G3 tumours and carcinoma in situ were aneuploid (61%, 79% and 100%, respectively). There were 33 cases of newly diagnosed G2 tumours, all treated by transurethral resection; 11 tumours were diploid, whereas the aneuploid cases could be divided into two distinct populations on the basis of the DNA-index (DI): I) 1.0〈DI≤ 1.5 and II) DI〉1.5. In group I a significantly lower number of cases (9%) showed tumour recurrence within 1 year, compared to group II (77%) (P=0.001; Fisher's test). This could not be explained solely by differences in tumour stage. G2 tumours of a higher stage (at least P1b) showed more early recurrences compared to lower stage tumours (P=0.02). In cases of discrepancies, however, the degree of aneuploidy, was found highly predictive for early tumour recurrence. Flow cytometric analysis of DNA content offers additional information for the recognition of rapidly recurring G2 bladder tumours allowing early installation of appropriate therapy.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00326962
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 2
    Electronic Resource
    Electronic Resource
    The use of computers for quantitative cell analysis (1990)
    Springer
    World journal of urology 8 (1990), S. 154-158 
    Details
    ISSN: 1433-8726
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary Computers are extensively employed in the quantitative analysis of cells and tissues using flow cytometry and/or image analysis. The first issue discussed in this paper is the use of computer-controlled multiparameter flow cytometry, with special emphasis on the use of pulse-processing techniques for DNA ploidy analysis. Subsequently, modern image analysis using a solid-state camera is discussed. Examples of automated analysis of Feulgen-DNA stained cells (absorption) or cells stained by in situ hybridization procedures (fluorescence) for defined DNA or RNA sequences are given. Finally, a new user-friendly microscope-computer interface (HOME=highly optimized microscope environment) is presented, in which the monitor of a personal computer is optically overlaid with the microscopic image, enabling the immediate reading of patient record data while viewing through the microscope. When equipped with a simple encoding stage for position tracking, this system enables more accurate screening of cytological specimens and provides rapid interactive image analysis and morphometry.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01576762
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 3
    Electronic Resource
    Electronic Resource
    Automated DNA-image cytometry: a prognostic tool in infiltrating bladder carcinoma? (1987)
    Springer
    World journal of urology 5 (1987), S. 127-132 
    Details
    ISSN: 1433-8726
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary In a retrospective study, paraffin-embedded cystectomy specimens obtained from 46 patients with bladder cancer (stage pT1 — pT4a, pN0, pN2) were analysed for tumor DNA ploidy and proliferation using automated image cytometry (LEYTAS). In 41 cases, DNA ploidy could be measured. Estimation of proliferation was possible in 26 tumors. The number of cells with a DNA content higher than 5C could be calculated in 38 of the tumors. All these three parameters are shown to correlate with patient outcome.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00327070
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 4
    Electronic Resource
    Electronic Resource
    Molecular cytogenetics: unraveling of the genetic composition of individual cells by fluorescence in situ hybridization and digital imaging microscopy (1995)
    Springer
    World journal of urology 13 (1995), S. 138-142 
    Details
    ISSN: 1433-8726
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary Molecular biology techniques allow the unraveling of the genetic alterations that cause or accompany malignant disease. Since tumors are often heterogeneous, biochemical analysis of tissue homogenates is of limited diagnostic value. This paper gives examples of methods that are presently operational to analyze the genetic composition of individual cells. They are based on fluorescence in situ hybridization (FISH) and digital imaging microscopy. First, the current status of indirect and direct FISH staining methods with respect to probe labeling, detection sensitivity, multiplicity, and DNA resolution is summarized. Microscope hardware as well as charge-coupled device (CCD) cameras required for FISH analysis are then described. Applications potentially important for the analysis of urological malignancies, such as the automated enumeration of chromosomal abnormalities (counting of dots in interphase cells) and high-resolution DNA mapping on highly extended chromatin, are described in detail. Finally, the limitations of the present methodology and its future prospects are discussed.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00184867
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 5
    Electronic Resource
    Electronic Resource
    Localized renal-cell carcinoma: detection of abnormal cells in peritumoral tissue (1995)
    Springer
    World journal of urology 13 (1995), S. 149-152 
    Details
    ISSN: 1433-8726
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary To spare organ function, partial resection of early diagnosed renal-cell carcinoma (RCC) is applied for well-localized and small-volume RCC with increasing frequency, although recurrence of the tumor in the same kidney is occasionally observed. The aim of the present study was to establish objective prognostic parameters that would allow the selection of tumors suitable for an organ-saving procedure. Of the 160 patients undergoing a radical nephrectomy, 67 were included in this study. In 7/45 patients with lymph-node dissection (15.6%), clinical staging revealed a false-negative lymph-node status. By means of conventional histopathology, multifocality could be demonstrated in 2/67 patients (3%); in 1/67 patients (1.5%), the ipsilateral adrenal gland was unexpectantly tumor-involved. Both tumor tissue and normal peritumoral tissue were examined for the presence of premalignant and tumor cells on the basis of DNA ploidy and of the expression of the tumor-associated G250 antigen, which is specifically expressed at the surface of renal cancer cells. In 40/67 (59.1%) peritumoral tissue specimens, cells with an abnormal DNA content could be observed using automated image analysis. In 12/67 cases (18%), cells obtained from peritumoral tissue also showed an aneuploid DNA histogram; 4/67 (6%) had a tumor-correlated DNA ploidy. Additionally, 38/67 (56.9%) of these tissues, histopathologically classified as normal, contained cells expressing the G250 antigen. These observations were independent of the stage or histological grade of the tumor. These data indicate that classic pathological parameters for tumor staging are insufficient for the detection of multifocality, occurring in more than 15% of cases. Additionally, it was shown that examination of tissue adjacent to the RCC allowed a specific detection of abnormal cells revealing abnormal ploidy or altered expression of tumor-associated antigens as compared with normal renal tissue in nearly 60% of cases investigated. The clinical relevance of this observation remains to be determined.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00184869
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 6
    Electronic Resource
    Electronic Resource
    Changes in erythropoiesis due to radiation or chemotherapy as studied by flow cytometric determination of peripheral blood reticulocytes (1986)
    Springer
    Histochemistry and cell biology 84 (1986), S. 544-548 
    Details
    ISSN: 1432-119X
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary Flow cytometric determination of time dependent changes of numbers of reticulocytes in peripheral blood were investigated as a parameter for changes in erythropoiesis induced by radiation- or chemotherapy. Rats irradiated or treated with drugs (such as e.g. cyclo-phosphamide 100 mg/kg, vincristin 0.2 mg/kg, or mitomycin C 1.0 mg/kg) showed clear changes in erythropoietic activity. Reticulocyte numbers decreased rapidly until day 3–4 after treatment; this period was followed by a gradual increase and normal control values were seen at day 8–11. Radiation effects of doses as low 0.5 Gy could be detected in such a way. Similar studies were performed with patients with ovarian tumors treated with cis-platinum, a drug that may cause non-immune haemolysis. During prolonged treatment some patients showed increasing numbers of reticulocytes, measured at the first day of each hospitalization period, whereas leucocyte and platelet counts stayed more or less constant. Increasing numbers of reticulocytes generally indicates stimulation of erythropoietic activity of the bone marrow (due to increased blood loss); in this study increasing numbers often preceeded a decrease in hemoglobin values later on. Flow cytometric analysis of reticulocytes is therefore a potentially useful tool to detect changes in erythropoiesis, and considered more sensitive for the early recognition of patients that develop anemia, than hemoglobin measurements only.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00482989
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 7
    Electronic Resource
    Electronic Resource
    Results of the automated analysis of 328 bladder specimens using the Leyden television analysis system (LEYTAS) (1983)
    Springer
    World journal of urology 1 (1983), S. 77-81 
    Details
    ISSN: 1433-8726
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary A computer-controlled image-analysis system (LEYTAS) was used to classify 328 cytological urinary specimens automatically into positive and negative. Classification was based on the presence of cells with increased DNA content or high chromatin contrast. These cells are automatically detected by the LEYTAS system and stored in image memories. Fully automated slide classification on the basis of the total number of detected cells resulted in 33% false-positives and 2% false-negatives. Positively classified preparations were then further investigated by rapid visual evaluation of the image memories in order to eliminate any detected artifacts. After this procedure the results were: 12% false-positives and 4% false-negatives. Automated screening of urinary cytology specimens for the detection of bladder cancer, therefore, is feasible. Further analysis of the unbiasedly detected cells for parameters, such as chromatin distribution and nuclear shape, provides a way to gain objective information with respect to grading, therapy, and prognsois.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00326747
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 8
    Electronic Resource
    Electronic Resource
    Reflection contrast microscopy (1988)
    Springer
    Histochemistry and cell biology 89 (1988), S. 141-150 
    Details
    ISSN: 1432-119X
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary Reflection contrast microscopy (RCM) has proven to be a useful tool for the study of living cells (Ploem 1975). Due to the effective suppression of aspecific reflected light by polarization optics combined with a quarter lambda plate at the front lens of the objective, low intensity reflection signals originating from minor amounts of precipitated diaminobenzidine (DABox) in immunocytochemically stained specimens, can be made visible. RCM has been successfully applied in demonstrating single copy nucleic acid sequences using in situ hybridization procedures (Landegent et al. 1984). We have systematically studied the aspects of image formation of DABox by RCM by using a model system consisting of glass slides coated with peroxidase containing protein layers to determine the conditions for optimal sensitivity of this detection method. Moreover, investigations were performed to study the relationship between the amount of reflected light and DABox depending on the thickness of the object. Both theoretical and practical evidence is obtained to show that DABox detection by RCM is based on interference phenomena occurring in the layer of DABox, and less on selective reflection. This restricts the type of specimen which can be used for sensitive detection of DABox by RCM. Consequently, in ultrathin (40 nm) sections osmificated DABox was visualized in peroxidatic positive cell organelles with high contrast and resolution. Similar results were obtained with immunoperoxidase stained material embedded in Lowicryl under conditions that did not allow visualization of the staining product by bright field microscopy.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00489917
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 9
    Electronic Resource
    Electronic Resource
    Improved detection and quantification of the (immuno) peroxidase product using reflection contrast microscopy (1989)
    Springer
    Histochemistry and cell biology 92 (1989), S. 153-160 
    Details
    ISSN: 1432-119X
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary Reflection contrast microscopy (RCM) is a sensitive tool to detect minor amounts of precipitated diaminobenzidine (DABox) in immunoperoxidase stained specimens. One of the main issues in immunocytochemistry is the ongoing need for more sensitive and quantitative techniques. Therefore we applied RCM, using a new simple model system, to methods previously described for increased sensitivity in immunocytochemistry with bright field microscopy. Addition of imidazole was found the most sensitive method and addition of Nickel and Cobalt ions gave the most enhanced colour intensity. Variation of the enzyme reaction parameters yielded a continuous increase in reflection with time. This was then discussed in view of other model studies of peroxidase kinetics. A quantitative relationship between the amount of peroxidase and the reflection of DABox was observed, indicating that quantitative immunoperoxidase studies with RCM are feasible. In situ hybridization (ISH) was then used as a useful biological model for RCM to test the optimal conditions for DAB staining found in the model system (high concentrations of DAB and peroxidase and 2 h incubation time). There was no background staining in the model system, also after prolonged incubation time. The ISH experiments showed that the contrast (ratio) between specific signal and chromosome background did not increase in time, whereas only the use of high avPO concentrations yielded the highest contrast.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00490236
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 10
    Electronic Resource
    Electronic Resource
    Synthesis and purification of horseradish peroxidase-labeled oligonucleotides for tyramide-based fluorescence in situ hybridization (2000)
    Springer
    Histochemistry and cell biology 113 (2000), S. 175-180 
    Details
    ISSN: 1432-119X
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Abstract  A method is presented to conjugate horseradish peroxidase (HRP) to oligodeoxynucleotides for fluorescence in situ hybridization assays employing tyramide signal amplification (TSA). HRP is covalently bound to the oligonucleotide by thiol ether linkage and purified by high-performance liquid chromatography. With TSA detection, a single HRP-labeled oligonucleotide probe is sufficient for in situ detection of clustered DNA repeat sequences with a degree of repetition between 20 and 50.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s004180050436
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
Close ⊗
This website uses cookies and the analysis tool Matomo. More information can be found here...