ISSN:
1432-2048
Keywords:
5-Azacytidine
;
DNA methylation
;
DNA (tandem repeats)
;
Nicotiana (tissue culture)
;
Plant regeneration (tobacco)
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
Notes:
Abstract Previously, we established experimental conditions allowing us to induce hypomethylation of tandem arrays of highly repetitive DNA sequences in the Nicotiana tabacum L. nuclear genome (M. Bezděk et al. 1991, Planta 184, 487–490). In this paper, we demonstrate that loci containing the highly repetitive sequences of the HRS60 family can maintain the induced hypomethylated state through protoplast regeneration, non-differentiated callus growth, and plant regeneration. The hypomethylation, induced with 5-azacytidine and monitored on these sequences, did not substantially alter the capacity of calli to produce plants. It can be concluded that, in contradistinction of multiple copies of transgenes or ectopic genes which are usually recognized as methylation targets, endogenous tandem repeats, such as the HRS60, present at 105 copies in the genome, can escape de-novo methylation.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF00197529
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