ISSN:
0749-1581
Keywords:
1H NMR
;
Casein phosphopeptide
;
2D NMR
;
COSY
;
HOHAHA
;
NOESY
;
β-Turn
;
Chemistry
;
Analytical Chemistry and Spectroscopy
Source:
Wiley InterScience Backfile Collection 1832-2000
Topics:
Chemistry and Pharmacology
Notes:
The solution conformation of β-casein phosphopeptide (CPP) was studied by 1H NMR spectroscopy. As a prerequisite to the conformational analysis the spectral assignment was carried out for CPP in the Ca2+-free and -bound states by the use of two-dimensional NMR spectroscopy. The assigned resonances were used for (1) pH-titration experiments, (2) elucidation of the temperature dependence of the amide resonance and (3) a nuclear Overhauser enhancement (NOE) experiment. Although the β-turn conformation is predicted for the sequence - Val8-Pro9-Gly10-Glu11-of CPP, the present NMR results do not provide favourable evidence for its formation.
Additional Material:
3 Ill.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1002/mrc.1260291105
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