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  • DNA fingerprinting  (1)
  • Digoxigenin  (1)
  • Typhoid fever  (1)
  • laminar flow  (1)
Materialart
Erscheinungszeitraum
  • 1
    Digitale Medien
    Digitale Medien
    Springer
    European journal of epidemiology 7 (1991), S. 77-82 
    ISSN: 1573-7284
    Schlagwort(e): Listeria monocytogenes ; Ribosomal DNA fingerprinting ; Nonisotopic DNA probe ; Digoxigenin
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Medizin
    Notizen: Abstract A nonisotopic ribosomal DNA fingerprinting technique was developed for the characterization of Listeria monocytogenes. Plasmid pKK3535 (a pBR322-derived plasmid containing rrnB ribosomal RNA operon of Escherichia coli) was labeled with digoxigenin-II-dUTP by random priming and used to probe EcoRI fragments of L. monocytogenes chromosomal DNA on nylon filters. The method was successfully applied to the characterization of two sets of patient and food isolates of L. monocytogenes.
    Materialart: Digitale Medien
    Bibliothek Standort Signatur Band/Heft/Jahr Verfügbarkeit
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  • 2
    Digitale Medien
    Digitale Medien
    Springer
    European journal of epidemiology 7 (1991), S. 192-193 
    ISSN: 1573-7284
    Schlagwort(e): Typhoid fever ; S. typhi ; DNA fingerprinting
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Medizin
    Materialart: Digitale Medien
    Bibliothek Standort Signatur Band/Heft/Jahr Verfügbarkeit
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  • 3
    Digitale Medien
    Digitale Medien
    Chichester : Wiley-Blackwell
    International Journal for Numerical Methods in Fluids 22 (1996), S. 283-296 
    ISSN: 0271-2091
    Schlagwort(e): laminar flow ; numerical modelling ; particle image velocimetry measurement ; rotating disc ; Engineering ; Engineering General
    Quelle: Wiley InterScience Backfile Collection 1832-2000
    Thema: Maschinenbau
    Notizen: The fluid flow field within an enclosed cylindrical chamber with a rotating flat disc was calculated using a finite volume computational fluid dynamics (CFD) model and compared with particle image velocimetry (PIV) measurements. Two particular laminar cases near the Transitional flow regime were investigated: Reynolds number Re=2.5×1 4, chamber aspect ratio G (h/Rd)=0.2 and Re=4.2×104, G (h/Rd)=0.217. This enabled direct comparison with the numerical and experimental results reported by other researchers. The computational details and some major factors that affect the computed accuracy and convergence speed are also discussed in detail. PIV results containing some 4300 velocity vector points in each of seven planes for each case were obtained from the flow field parallel to the rotating disc. It was found that PIV results could be obtained in planes within the boundary layers as well as the core flow by careful use of a thin laser illumination sheet and correct choice of laser pulse separation. There was close agreement between numerical results, the present PIV measurements and other reported experimental and numerical results.
    Zusätzliches Material: 10 Ill.
    Materialart: Digitale Medien
    Bibliothek Standort Signatur Band/Heft/Jahr Verfügbarkeit
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