ISSN:
1573-7284
Keywords:
Listeria monocytogenes
;
Ribosomal DNA fingerprinting
;
Nonisotopic DNA probe
;
Digoxigenin
Source:
Springer Online Journal Archives 1860-2000
Topics:
Medicine
Notes:
Abstract A nonisotopic ribosomal DNA fingerprinting technique was developed for the characterization of Listeria monocytogenes. Plasmid pKK3535 (a pBR322-derived plasmid containing rrnB ribosomal RNA operon of Escherichia coli) was labeled with digoxigenin-II-dUTP by random priming and used to probe EcoRI fragments of L. monocytogenes chromosomal DNA on nylon filters. The method was successfully applied to the characterization of two sets of patient and food isolates of L. monocytogenes.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF00221345
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