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  • 1
    Electronic Resource
    Electronic Resource
    Genetic aspects of the organization of legumin genes in pea (1993)
    Springer
    Plant molecular biology 22 (1993), S. 101-112 
    Details
    ISSN: 1573-5028
    Keywords: pea ; Pisum sativum ; recombination ; physical & genetic maps ; legumin
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract We have compared physical and genetic maps of the region around the legJ gene in pea. In this vicinity there are four B-type legumin genes, arranged as two close pairs. The detection of a recombination event within this gene cluster allows the orientation of this group of genes within the surrounding linkage group to be determined. The relationship between physical and genetic distances in this region is discussed, as are the implications of this for relating physical and genetic maps elsewhere in the pea genome.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00038999
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    Paper (German National Licenses)
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  • 2
    Electronic Resource
    Electronic Resource
    A copia-like element in Pisum demonstrates the uses of dispersed repeated sequences in genetic analysis (1990)
    Springer
    Plant molecular biology 15 (1990), S. 707-722 
    Details
    ISSN: 1573-5028
    Keywords: retrotransposon ; Pisum ; segregation ; PCR ; RFLP ; mapping
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract A DNA sequence between two legumin genes in Pisum is a member of the copia-like class of retrotransposons and represents one member of a polymorphic and heterogeneous dispersed repeated sequence family in Pisum. This sequence can be exploited in genetic studies either by RFLP analysis where several markers can be scored together, or the segregation of individual elements can be followed after PCR amplification of specific members.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00016121
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    Paper (German National Licenses)
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  • 3
    Electronic Resource
    Electronic Resource
    Repeated sequences as genetic markers in pooled tissue samples (1993)
    Springer
    Plant molecular biology 22 (1993), S. 153-157 
    Details
    ISSN: 1573-5028
    Keywords: linkage ; DNA mixtures ; repeated sequences ; pea ; Pisum sativum
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract We show, using the PDR1 element of pea, that dispersed repeated sequences of moderate copy number can be used simply and efficiently to generate markers linked to a trait of interest. Inspection of hybridization patterns of repeated sequences to DNA mixtures of pooled genotypes is a sensitive way of detecting such markers. The large number of bands in tracks of digests of these mixtures allows the simultaneous sampling of loci at many places in the genome, and the many unlinked loci serve as internal controls. It is also shown that intensity ratios calculated from these band differences can be used to give a rough estimate of linkage distance.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00039003
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    Paper (German National Licenses)
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  • 4
    Electronic Resource
    Electronic Resource
    An RFLP marker for rb in pea (1988)
    Springer
    Theoretical and applied genetics 75 (1988), S. 362-365 
    Details
    ISSN: 1432-2242
    Keywords: Pea ; r b ; RFLP ; Vicilin gene
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary We have located an RFLP marker, corresponding to the locus Vc-5, which is linked to the r b locus. We also show that the heterogeneity at the Vc-5 locus is less among r brb lines than among pea genotypes as a whole. The relevance of this RFLP is discussed in relation to the construction of the double recessive rr r brb genotypes.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00303978
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  • 5
    Electronic Resource
    Electronic Resource
    Pea Ty1-copia group retrotransposons: transpositional activity and use as markers to study genetic diversity in Pisum (2000)
    Springer
    Molecular genetics and genomics 263 (2000), S. 898-907 
    Details
    ISSN: 1617-4623
    Keywords: Key words Retrotransposon ; Genetic diversity ; Molecular taxonomy ; Pea ; DNA markers
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The variation in transposition history of different Ty1-copia group LTR retrotransposons in the species lineages of the Pisum genus has been investigated. A heterogeneous population of Ty1-copia elements was isolated by degenerate PCR and two of these (Tps12 and Tps19) were selected on the basis of their copy number and sequence conservation between closely related species for further in-depth study of their transpositional history in Pisum species. The insertional polymorphism of these elements and the previously characterised PDR1 element was studied by sequence-specific amplification polymorphism (SSAP). Each of these elements reveals a unique transpositional history within 55 diverse Pisum accessions. Phylogenetic trees based on the SSAP data show that SSAP markers for individual elements are able to resolve different species lineages within the Pisum genus. Finally, the SSAP data from all of these retrotransposon markers were combined to reveal a detailed picture of the intra and inter-species relationships within Pisum.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s004380000257
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    Paper (German National Licenses)
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