ZIB

Hits per page

hits 1 - 2 | 2 hits

Sorting

Electronic Resource

Identification and expression of the Neurospora crassa mei-3 gene which encodes a protein homologous to Rad51 of Saccharomyces cerevisiae (1995)

Hatakeyama, Shin ; Ishii, Chizu ; Inoue, Hirokazu

Springer

Molecular genetics and genomics 249 (1995), S. 439-446

add to mindlist on the mindlist

Details

ISSN: 1617-4623

Keywords: Neurospora crassa ; mei-3 ; RecA ; Rad51 ; DNA-recombination

Source: Springer Online Journal Archives 1860-2000

Topics: Biology

Notes: Abstract The mei-3 gene of Neurospora crassa encodes a homolog of the Escherichia coli RecA and Saccharomyces cerevisiae Rad51 proteins, which are required for recombination and repair of DNA double-strand breaks. To determine the molecular function of MEI3 protein, anti-MEI3 antibody was prepared and used in Western blot analysis. The antibody cross-reacted only with crude extracts prepared from perithecia, the fruiting bodies of Neurospora. The molecular weight of the MEI3 protein was estimated to be 38 kDa. Transformation experiments showed that a DNA fragment longer than previously reported was needed to complement the mei-3 mutation. On sequencing cDNA and genomic DNA, one open reading frame (ORF) was found, which consists of three exons interrupted by two small introns. This ORF encoded a MEI3 protein of 353 amino acids, and the inferred MW of 38 kDa is in good agreement with the results from Western blot analysis. Comparisons of MEI3 with other Rad51 homologs indicated that MEI3 protein contains the two conserved core domains (I and II) generally observed in Rad51 homologs in eukaryotes. Northern blot analysis showed that expression of mei-3 was raised remarkably after UV-irradiation or methyl methanesulfonate (MMS)-treatment. The transcript size was 1.6 kb and this was also larger than was reported previously.

Type of Medium: Electronic Resource

URL: http://dx.doi.org/10.1007/BF00287106

Permalink

Library	Location	Call Number	Volume/Issue/Year	Availability

Others were also interested in ...

Paper (German National Licenses)

Fulltext

Electronic Resource

Characterization of flat revertants isolated from cells transformed by Abelson murine leukemia virus (Ab-MuLV) (1990)

Oka, Takashi ; Yutsudo, Masuo ; Inoue, Hirokazu ; [et al.]

New York, NY [u.a.] : Wiley-Blackwell

Journal of Cellular Physiology 145 (1990), S. 428-433

add to mindlist on the mindlist

Details

ISSN: 0021-9541

Keywords: Life and Medical Sciences ; Cell & Developmental Biology

Source: Wiley InterScience Backfile Collection 1832-2000

Topics: Biology , Medicine

Notes: Transformed Fisher rat fibroblast cell lines by Abelson murine leukemia virus frequently revert to the normal phenotype in usual culture conditions. Molecular Biological analysis of thre revertant clones isolated from the transformants showed that their morphological reversions were due to inactivation of the v-abl oncogene at multiple steps including transcription, translation or v-abl protein kinase activity itself without any change in structural gene expression of helper virus. These findings suggest the existence of a specific mechanism(s) for elimination of the v-abl oncogene by segregation, mutation, or gene rearrangement in these cells.

Additional Material: 5 Ill.