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  • Polymer and Materials Science  (8)
  • Datura  (4)
  • Feedback regulation  (2)
  • agarose  (2)
  • 1
    Electronic Resource
    Electronic Resource
    Introduction of resistance to kanamycin into the protoplasts from a pantothenate-requiring auxotrophic cell line of Datura innoxia P. Mill. via direct gene transfer
    Amsterdam : Elsevier
    Plant Science 70 (1990), S. 105-114 
    Details
    ISSN: 0168-9452
    Keywords: Datura ; auxotrophy ; direct gene transfer ; kanamycin-resistance ; protoplasts ; universal hybridizer
    Source: Elsevier Journal Backfiles on ScienceDirect 1907 - 2002
    Topics: Biology
    Type of Medium: Electronic Resource
    URL: http://linkinghub.elsevier.com/retrieve/pii/0168-9452(90)90038-P
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  • 2
    Electronic Resource
    Electronic Resource
    The selection of chlorsulfuron-resistant cell lines of independent origin from an embryogenic cell suspension culture of Brassica napus L.
    Amsterdam : Elsevier
    Plant Science 69 (1990), S. 231-237 
    Details
    ISSN: 0168-9452
    Keywords: Brassica napus ; agarose ; chlorsulfuron ; clonal cultures ; embryogenesis ; regeneration ; resistance
    Source: Elsevier Journal Backfiles on ScienceDirect 1907 - 2002
    Topics: Biology
    Type of Medium: Electronic Resource
    URL: http://linkinghub.elsevier.com/retrieve/pii/0168-9452(90)90122-5
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  • 3
    Electronic Resource
    Electronic Resource
    Somatic hybrids derived from fusion of a universal hybridizer and a wild type line of Datura
    Amsterdam : Elsevier
    Plant Science 79 (1991), S. 111-118 
    Details
    ISSN: 0168-9452
    Keywords: Datura ; auxotrophy ; cell culture ; kanamycin resistance ; somatic hybrid ; universal hybridizer
    Source: Elsevier Journal Backfiles on ScienceDirect 1907 - 2002
    Topics: Biology
    Type of Medium: Electronic Resource
    URL: http://linkinghub.elsevier.com/retrieve/pii/0168-9452(91)90077-L
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  • 4
    Electronic Resource
    Electronic Resource
    Transplantation of isolated nuclei into plant protoplasts (1986)
    Springer
    Planta 168 (1986), S. 29-35 
    Details
    ISSN: 1432-2048
    Keywords: Auxotrophy ; Datura ; DNA transfer ; Nuclear transplantation ; Protoplast ; Prototrophy ; Vicia (nuclear transplantation)
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The uptake of isolated nuclei from Vicia hajastana Grossh. cells into protoplasts of an auxotrophic cell line of Datura innoxia P. Mill. was induced under the influence of polyethylene glycol and Ca2+ at pH 6.8. The frequency of nuclear uptake varied from 0.8 to 2.3% and that of the recovery of prototrophic clones from 10-5 to 6·10-4. The prototrophic nuclear fusion products following nuclear uptake could be rescued by initial culture of the protoplasts in non-selective conditions and by the subsequent use of feeder cell layers to support the growth of surviving colonies on a selective medium. The presence of Vicia genomic DNA in some prototrophic clones was confirmed by dot-blot hybridization using Datura and Vicia DNA probes. In certain transformed clones, the recovery of prototrophy was accompanied by the restoration of morphogenetic potential. Welldeveloped shoots typical of wild-type Datura could be regenerated employing an appropriate regeneration medium.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00407005
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  • 5
    Electronic Resource
    Electronic Resource
    A valine-resistant mutant ofArabidopsis thaliana displays an acetolactate synthase with altered feedback control (1994)
    Springer
    Planta 192 (1994), S. 249-255 
    Details
    ISSN: 1432-2048
    Keywords: Acetolactate synthase ; Amino acid (branched chain) ; Arabidopsis ; Feedback regulation ; Mutant ; Valine resistance
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract A valine-resistant mutant line, VAL-2, ofArabidopsis thaliana (L.) Heynh. was identified by screening M 2 populations of ethylmethane-sulfonate-mutagenized seeds. The resistance was found to be due to a single, dominant, nuclear gene mutation. Assay of acetolactate synthase (ALS) indicated that the valine resistance in this mutant is caused by decreased sensitivity of ALS to the branched-chain amino acids, valine, leucine andisoleucine. A two fold decrease in apparentK m value for pyruvate of the mutant ALS enzyme was detected compared with that of the wild type. The sensitivity of the ALS enzyme to sulfonylurea, imidazolinone and triazolopyrimidine herbicides was not altered in the mutant. At the plant growth level the mutant was also resistant to valine plus leucine, but was sensitive to leucine orisoleucine alone. The mutant gene,var1, maps, or is very closely linked, toCSR1, the gene encoding acetolactate synthase inArabidopsis.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01089041
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  • 6
    Electronic Resource
    Electronic Resource
    A valine-resistant mutant of Arabidopsis thaliana displays an acetolactate synthase with altered feedback control (1994)
    Springer
    Planta 192 (1994), S. 249-255 
    Details
    ISSN: 1432-2048
    Keywords: Acetolactate synthase ; Amino acid (branched chain) ; Arabidopsis ; Feedback regulation ; Mutant ; Valine resistance
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract A valine-resistant mutant line, VAL-2, of Arabidopsis thaliana (L.) Heynh. was identified by screening M 2 populations of ethylmethane-sulfonate-mutagenized seeds. The resistance was found to be due to a single, dominant, nuclear gene mutation. Assay of acetolactate synthase (ALS) indicated that the valine resistance in this mutant is caused by decreased sensitivity of ALS to the branched-chain amino acids, valine, leucine and isoleucine. A two fold decrease in apparent K m value for pyruvate of the mutant ALS enzyme was detected compared with that of the wild type. The sensitivity of the ALS enzyme to sulfonylurea, imidazolinone and triazolopyrimidine herbicides was not altered in the mutant. At the plant growth level the mutant was also resistant to valine plus leucine, but was sensitive to leucine or isoleucine alone. The mutant gene, var1, maps, or is very closely linked, to CSR1, the gene encoding acetolactate synthase in Arabidopsis.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00194459
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  • 7
    Electronic Resource
    Electronic Resource
    A transient duplication of the acetolactate synthase gene in a cell culture of Datura innoxia (1987)
    Springer
    Theoretical and applied genetics 74 (1987), S. 417-422 
    Details
    ISSN: 1432-2242
    Keywords: Gene amplification ; Datura ; Sulfonylurea resistance ; Plant cell culture
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary A 2.0 kb fragment of the yeast ILV2 gene, which codes for the target enzyme acetolactate synthase (ALS) of the herbicide chlorsulfuron, was shown to hybridize to the nuclear DNA of a haploid cell culture of Datura innoxia P. Mill. Nuclear DNA of a chlorsulfuron resistant line of D. innoxia, CSR6, gave a prominent 2.65 kb band when cleaved by either EcoRI or HindIII. The 2.65 kb band has been shown to hybridize with the yeast ILV2 probe. A herbicide resistant line descended from CSR6 by continuous culture resulted in the loss of the 2.65 kb restriction fragment. These observations suggest that CSR6 resulted from a large tandem duplication of the ALS gene and that a point mutation for herbicide resistance in an ALS gene repeat unit of the duplication was selected during subsequent growth of the resistant line.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00289814
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  • 8
    Electronic Resource
    Electronic Resource
    Isolation and culture of Lens culinaris Medik. cv. Eston epicotyl protoplasts to calli (1990)
    Springer
    Plant cell, tissue and organ culture 20 (1990), S. 75-79 
    Details
    ISSN: 1573-5044
    Keywords: Lens culinaris ; protoplasts ; osmoticum ; agarose ; callus formation
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Protoplasts of Lens culinaris Medik. cv. Eston were isolated from epicotyl tissues of seedlings grown on Murashige & Skoog basal medium. For isolating the protoplasts, epicotyl tissues were digested for 12–14 h at 25°C in an isolation mixture (pH 5.7) containing 1% Cellulase RS, 0.5% Driselase, 0.25% Pectolyase Y23, 0.2M calcium chloride, 10 mM mannitol and 10 mM MES. Protoplasts were purified by flotation over 20% sucrose and washed with 0.2 M calcium chloride solution supplemented with 10 mM mannitol. Purified protoplasts were cultured at a density of 105 ml-1 in agarose (Seaplaque, 0.6%) blocks which were suspended in an identical but liquid KM8P culture medium lacking amino acids, ammonium nitrate, and coconut water but containing 0.35 M glucose and a growth regulator complement of either 2.2 μM 2,4-dichlorophenoxyacetic acid (2,4-D), 2.7 μM naphthaleneacetic acid (NAA), 2.3 μM N-(2-furanylmethyl)-1H-purine-6-amine (kinetin), 2.2 μM benzylamino purine (BAP), 2.3 μM 2-methyl-4-(1H-purine-6-ylamino)-2-buten-1-ol (zeatin), and 1.4 μM gibberellic acid (GA3), or 5.4 μM NAA and 2.2 μM each of 2,4-D and BAP. The osmotic potential of the liquid culture medium was gradually reduced over a period of 3 weeks by replacing the spent medium with a fresh medium containing 0.25, 0.1 and 0 M glucose at weekly intervals. About 6% of the dividing protoplasts developed into cell colonies after 3 weeks of culture at 25°C in diffuse light (10 μE m-2s-1). In 35–42 days the microcolonies were about 1 mm in diameter and developed into calli on transfer to agar-solidified B5 medium supplemented with growth regulators used in the protoplast culture medium and 5 mM glutamine. Attempts to regenerate plants from protoplast-derived calli have so far been unsuccessful.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00034762
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  • 9
    Electronic Resource
    Electronic Resource
    Molecular orientation and young's modulus of plasticized and nonplasticized poly(vinyl chloride) (1988)
    New York, NY [u.a.] : Wiley-Blackwell
    Journal of Applied Polymer Science 35 (1988), S. 787-796 
    Details
    ISSN: 0021-8995
    Keywords: Chemistry ; Polymer and Materials Science
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology , Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics , Physics
    Notes: The Young's moduli of samples of poly(vinyl chloride) containing either no plasticizer or 5 pph of dioctyl sebacate were drawn uniaxially at 80°C to draw ratios, λ, in the range 1-3.3. The moduli, determined by a three-point bending method were found to increase monotonically with λ to a value of 6.3 GPa for the nonplasticized samples and to reach a maximum value of about 4.5 GPa for the plasticized samples above λ = 2.3, which showed voiding. The lower moduli of the plasticized samples for a given degree of orientation, as assessed from birefringence measurements, can be attributed to the diluent effect of the plasticizer on the load-bearing chains. The Raman measurements suggest that the crystallites probably orient rather like rigid rods in an affinely deforming matrix, with some relaxation.
    Additional Material: 6 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/app.1988.070350318
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  • 10
    Electronic Resource
    Electronic Resource
    Studies on the adhesion of polyimide coatings on copper foil (1992)
    New York, NY [u.a.] : Wiley-Blackwell
    Journal of Applied Polymer Science 45 (1992), S. 947-956 
    Details
    ISSN: 0021-8995
    Keywords: Chemistry ; Polymer and Materials Science
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology , Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics , Physics
    Notes: The peel strength and the color of the copper foil peeled at 90 degrees from five different polyimide films were studied. The interfacial surfaces of copper foil and polyimide were examined by scanning electron microscopy (SEM), X-ray photoelectron spectroscopy (XPS), and energy dispersion analysis by X-ray (EDAX). There is a correlation between peel strength, and the color of the interfacial side copper caused by oxygen diffusion. Study of the imidization process carried out in vacuum indicates that the geometric arrangements of the atoms of polyimide also play a very important role in peel strength.
    Additional Material: 5 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/app.1992.070450602
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