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  • Mast cell  (3)
  • Deep-etching  (2)
  • [abr] RP-HPLC; reverse phase-high performance liquid chromatography  (2)
  • 1
    Electronic Resource
    Electronic Resource
    Expression of bovine lung prostaglandin F synthase in Escherichia coli
    Amsterdam : Elsevier
    Biochemical and Biophysical Research Communications 181 (1991), S. 272-278 
    Details
    ISSN: 0006-291X
    Keywords: [abr] 9α,l1β-PGF"2; ; [abr] CD; circular dichroism ; [abr] DTNB; 5,5-dithiobis (2-nitrobenzoic acid) ; [abr] E. coli; Eschericia coli ; [abr] PG; Prostaglandin ; [abr] RP-HPLC; reverse phase-high performance liquid chromatography ; [abr] SDS; sodium dodecyl sulfate
    Source: Elsevier Journal Backfiles on ScienceDirect 1907 - 2002
    Topics: Biology , Chemistry and Pharmacology , Physics
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1016/S0006-291X(05)81413-5
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    Paper (German National Licenses)
    Fulltext
  • 2
    Electronic Resource
    Electronic Resource
    Expression of bovine lung prostaglandin F synthase in Escherichia coli
    Amsterdam : Elsevier
    Biochemical and Biophysical Research Communications 181 (1991), S. 272-278 
    Details
    ISSN: 0006-291X
    Keywords: [abr] 9α,l1β-PGF"2; ; [abr] CD; circular dichroism ; [abr] DTNB; 5,5-dithiobis (2-nitrobenzoic acid) ; [abr] E. coli; Eschericia coli ; [abr] PG; Prostaglandin ; [abr] RP-HPLC; reverse phase-high performance liquid chromatography ; [abr] SDS; sodium dodecyl sulfate
    Source: Elsevier Journal Backfiles on ScienceDirect 1907 - 2002
    Topics: Biology , Chemistry and Pharmacology , Physics
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1016/S0006-291X(05)81413-5
    Library Location Call Number Volume/Issue/Year Availability
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    Paper (German National Licenses)
    Fulltext
  • 3
    Electronic Resource
    Electronic Resource
    X-ray microanalysis of rat mast cells stimulated with compound 48/80 in combination with quick-freezing method (1994)
    Springer
    Virchows Archiv 425 (1994), S. 435-438 
    Details
    ISSN: 1432-2307
    Keywords: Mast cell ; Quick-freezing ; Compound 48/80 ; X-ray microanalysis
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract X-ray microanalysis was performed on rat mast cells prepared by quick-freezing, cryosectioning and freeze-drying (QF-FD) method, or quick-freezing and freeze-substitution (QF-FS) method. Peritoneal cells including mast cells were stimulated with compound 48/80 for 0, 10 or 30 s at 17° C, and the mast cells stimulated for 30 s started exocytosis. In X-ray spectra of the QF-FD specimen, mast cells stimulated for 10 s increased their levels of phosphorus, sodium and chlorine in the intergranular cytoplasm prior to exocytosis, and kept this increase until 30 s after stimulation. In the QF-FS specimen, where soluble elements were removed, peaks of phosphorus, sulphur and potassium could be detected as elements in X-ray spectra. Phosphorus increased and potassium decreased in intergranular cytoplasm of mast cells stimulated for 10 s, and these changes became more obvious after 30 s. However, supplemental increase of other cations such as sodium could not be detected in the QF-FS specimens.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00189582
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    Paper (German National Licenses)
    Fulltext
  • 4
    Electronic Resource
    Electronic Resource
    Freeze-fracture immunocytochemistry for intracellular localization of serotonin in mast cells stimulated with compound 48/80 (1995)
    Springer
    Virchows Archiv 426 (1995), S. 267-270 
    Details
    ISSN: 1432-2307
    Keywords: Mast cell ; Compound 48/80 ; Exocytosis ; Freeze-fracture immunocytochemistry
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract Changes of intracellular localization of serotonin in rat mast cells were examined by freeze-fracture immunocytochemistry, to prevent the translocation of the serotonin antigen. Rat peritoneal cells including mast cells were stimulated in vitro with compound 48/80, at 17° C for 0, 30 or 60 s for exocytosis to occur. The mast cells were fixed, quickly frozen and freeze-fractured to expose the antigen on the fractured surface. They were immunostained with serotonin antibody, and the immunoreactions on the fractured surface were examined on ultrathin sections by electron microscopy. Unstimulated mast cells exhibited serotonin localization mostly in each intragranular matrix. In contrast, mast cells stimulated for 30 s exhibited increased serotonin in their intergranular cytoplasm. Mast cells showed more distinct immunoreactions in the cytoplasm where degranulation would be promoted after 60 s. It is suggested that intracellular release of serotonin occurred in the stimulated mast cells.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00191364
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    Paper (German National Licenses)
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  • 5
    Electronic Resource
    Electronic Resource
    Different organization of intermediate filaments in columnar cells of rat large intestinal mucosa as revealed by confocal laser scanning microscopy and quick-freezing and deep-etching method (1995)
    Springer
    Virchows Archiv 426 (1995), S. 401-410 
    Details
    ISSN: 1432-2307
    Keywords: Intermediate filament ; Columnar cell ; Large intestine ; Laser microscopy ; Deep-etching
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract The relationship between cell differentiation and ultrastructural changes of intermediate filaments (IF) was studied in columnar cells of large intestinal mucosa of rats by confocal laser scanning microscopy and quick-freezing and deep-etching method. A feature of the IF in immature columnar cells was minibundle formation with prominent branching, which organized the meshwork structures. The minibundles, which appeared to be formed by the attachment of two or more IF in side-to-side fashion, were loosely distributed throughout the cytoplasm. In contrast, in mature columnar cells, the IF were densely distributed under the terminal web in the cytoplasm and beneath the upper part of the lateral membrane regions, whereas the other areas of the cytoplasm contained only a small number of IF. Minibundle formation was not observed, and the branching was rarely identified. The changes in the distribution and density of IF, which are expressed in specific areas of mature columnar cells, apparently represent a characteristic of intracellular differentiation. It is suggested that the dissociation of minibundled IF, which was often observed in the immature columnar cells, is an important step in the acquisition of functional polarity in cells of this type.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00191350
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    Paper (German National Licenses)
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  • 6
    Electronic Resource
    Electronic Resource
    Dynamic structure of glomerular capillary loop as revealed by an in vivo cryotechnique (1996)
    Springer
    Virchows Archiv 427 (1996), S. 519-527 
    Details
    ISSN: 1432-2307
    Keywords: Quick-freezing ; Glomerulus ; Basement membrane ; Freeze-substitution ; Deep-etching
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract Morphological studies using immersion or perfusion fixation methods do not reveal the ultrastructure of functioning kidneys with normal circulation. A simple apparatus was developed for freezing the kidneys in vivo without stopping the blood supply, and the ultrastructure of the glomerular capillary loops was examined under different haemodynamic conditions. Mouse kidneys were frozen under normal blood flow conditions; others were frozen in the same way after ligation of the abdominal aorta at a point caudal to the renal arteries. They were then processed for the freeze-substitution or deep-etching method. Good ultrastructural preservation was obtained within about 5 μm depth from the frozen tissue surface. Functioning glomeruli with normal blood flow possessed open capillary lumens, different shapes of foot processes and atypical basement membranes with low density. Moreover, heterogeneity in width between foot processes was identified on the replica membranes. Under the acute conditions used to increase blood supply into the kidneys, the spaces between the flat foot processes became more widely dilated and the basement membrane was seen to be three-layered. The ultrastructure of glomeruli in functioning kidneys has been demonstrated for the first time by this “in vivo cryotechnique”.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00199513
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    Paper (German National Licenses)
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  • 7
    Electronic Resource
    Electronic Resource
    Ultrastructural study of mast cells stimulated with compound 48/80 as revealed by quick-freezing method (1994)
    Springer
    Virchows Archiv 424 (1994), S. 287-294 
    Details
    ISSN: 1432-2307
    Keywords: Mast cell ; Compound 48/80 ; Ultrastructure ; Quick-freezing
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract The ultrastructure of mast cells stimulated with compound 48/80 was examined by quick-freezing and deep-etching (QF-DE) or freeze-substitution (QF-FS) methods. Peritoneal cells including mast cells of adult male rats were stimulated in vitro with compound 48/80 at 17° C for 0, 10, 30, 60 or 180 s. The QF-DE replicas revealed that the mast cells stimulated with compound 48/80 for 30 s decreased filamentous actin around secretory granules. In the QF-FS specimens, perigranular membranes in mast cells stimulated for 60 s formed pentalaminar structures between adjacent granules in their cytoplasm prior to degranulation. These findings suggest that preparatory states for degranulation occur in the whole cytoplasm of stimulated mast cells at early stages. Moreover, both QF-FS specimens and QF-DE replicas revealed a compact morphological appearance of discharged granules in the extra-cellular space, indicating the existence of considerable content within the granules. Skeletal structures in the granules were also demonstrated on QF-DE replicas prepared after extracting soluble elements from the cytoplasm. It is suggested that the granular contents associated with the skeletal structures are gradually detached from the discharged granules to ensure local concentration in the tissues.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00194613
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    Paper (German National Licenses)
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