ISSN:
1573-5044
Keywords:
Dendranthema grandiflora syn. Chrysanthemum morifolium
;
surfactant
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
Notes:
Abstract Supplementation of MS-based medium containing 4.4 μM 6-benzyladenine and 2.7 μM α-naphthaleneacetic acid with 0.001–0.1% (w/v) of the non-ionic, co-polymer surfactant, Pluronic F-68, significantly (p〈0.05) increased the mean fresh weight gain of cultured leaf explants of chrysanthemum (Dendranthema grandiflora) “Tone Maid” and “Early Charm” by a maximum of 74% and 34%, respectively. The percentage of individual explants giving adventive shoots was also stimulated by Pluronic F-68; for cv. Early Charm, 0.001% (w/v) Pluronic F-68 induced the maximum response, whereas for cv. Tone Maid, the maximum response occurred with 0.01% surfactant. Shoot regeneration from explants was also enhanced at these concentrations of surfactant, compared to explants cultured in the absence of Pluronic F-68.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF00051576
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