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  • 1
    Electronic Resource
    Electronic Resource
    Acute physiological responses during blood substitution with colloidal gelatin in conscious rats (1984)
    Springer
    Cellular and molecular life sciences 40 (1984), S. 700-701 
    Details
    ISSN: 1420-9071
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary The effects of exchange-transfusion with a proprietary gelatin-based plasma volume expander, Haemaccel, have been investigated in conscious, chronically-catheterized rats. No adverse changes in basic cardiovascular or respiratory functions occurred during the procedure although an increase in intravascular fluid sodium but not potassium concentration was observed.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01949730
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  • 2
    Electronic Resource
    Electronic Resource
    Changes in plasma enzyme concentrations in response to blood substitution with perfluorocarbon emulsion in the conscious rat (1986)
    Springer
    Cellular and molecular life sciences 42 (1986), S. 1228-1231 
    Details
    ISSN: 1420-9071
    Keywords: Blood replacement ; perfluorocarbon emulsion ; Fluosol-DA ; lactate dehydrogenase ; alkaline phosphatase ; rats
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary The effects of near total blood replacement with the proprietary perfluorocarbon (PFC)-based emulsion, Fluosol-DA 20%, on plasma concentrations of 2 enzymes, lactate dehydrogeanse (LDH) and alkaline phosphatase (ALP), have been examined in conscious, chronically catheterized rats. A pronounced fall in both plasma LDH (p〈0.05) an ALP (p〈0.01) occurred in response to exchange-transfusion. However, at 6 h following blood replacement, plasma concentrations of both enzymes had risen to values significantly greater than those measured immediately before perfusion. The observed changes in plasma LDH and ALP after blood replacement with Fluosol-DA indicated alterations in normal functioning of tissues from which these enzymes originate.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01946398
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  • 3
    Electronic Resource
    Electronic Resource
    Changes in intravascular fluid composition following blood replacement with perfluorocarbon emulsion in the rat (1985)
    Springer
    Journal of molecular medicine 63 (1985), S. 1028-1034 
    Details
    ISSN: 1432-1440
    Keywords: Perfluorocarbons ; Blood replacement ; Adverse reactions ; Leukocytes
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary The effects of near total blood replacement with the proprietary perfluorocarbon emulsion, Fluosol-DA, have been examined in conscious, chronically catheterized rats. Animals survived for up to 48 h with a haematocrit of 〈2%. No overt changes in cardiovascular or respiratory functions occurred during blood replacement but thereafter, animals showed a progressive deterioration of haemodynamic control. Changes in intravascular fluid composition during and following exchange-transfusion have been monitored and the extent to which these changes could have contributed to the eventual disruption of homeostasis is discussed.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01737640
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  • 4
    Electronic Resource
    Electronic Resource
    Relationships in pineapple by random amplified polymorphic DNA (RAPD) analysis (2001)
    Oxford, UK : Blackwell Publishing Ltd
    Plant breeding 120 (2001), S. 0 
    Details
    ISSN: 1439-0523
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Random amplified polymorphic DNA (RAPD) analysis was applied to eight commercial cultivars of pineapple, two intergroup hybrids and two wild species. Morphologically, pineapple is divided into the Cayenne, Queen, Spanish, Maipure and Abacaxi groups. Members of the first three groups have been analysed in this study. The cultivars ‘Tradsithong’, ‘Phuket’, ‘Sawee’ and ‘Tainan’, with spiny leaves, form the Queen group. In ‘Pattavia’, ‘Nanglae’ and ‘Petburi no. 2’ (Cayenne group), spines are confined to the leaf tips. ‘Intrachitdang’ is normally placed in the Spanish group, which is morphologically similar to the Queen group, but with inferior quality fruit. DNA amplification products were compared from 16 arbitrary 10-mer primers from which a dendrogram was constructed. The results confirmed morphological classifications for seven of the eight commercial cultivars, with the Queen and Cayenne groups as separate clusters. However, the cv. ‘Intrachitdang’ was more closely related to the Cayenne group. Two hybrids from reciprocal Cayenne × Queen group crosses, were more closely allied to the Queen group. The two wild species were outside the groups. RAPD analysis can be exploited to investigate relationships within pineapple germplasm.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1046/j.1439-0523.2001.00606.x
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  • 5
    Electronic Resource
    Electronic Resource
    Effects of Pluronic F-68 on plant cells in suspension culture (1990)
    Springer
    Biotechnology letters 12 (1990), S. 29-32 
    Details
    ISSN: 1573-6776
    Source: Springer Online Journal Archives 1860-2000
    Topics: Process Engineering, Biotechnology, Nutrition Technology
    Notes: Summary The effects of the non-ionic surfactant, Pluronic F-68, on growth and structure ofSolanum dulcamara cells in suspension culture have been studied. Growth of cells, as measured by dry weight, was unaffected by low concentrations (0.01–1.0% w/v) of pluronic, while culture with higher concentrations (2.5–10.0%) resulted in cell death. It is suggested that low concentrations of pluronic may be valuable supplements in plant cell cultures to protect against mechanical damage and to manipulate membrane systems.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01028488
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  • 6
    Electronic Resource
    Electronic Resource
    Effects of Pluronic F-68 on larval development of the hookworm,Necator americanus (1991)
    Springer
    Biotechnology letters 13 (1991), S. 599-602 
    Details
    ISSN: 1573-6776
    Source: Springer Online Journal Archives 1860-2000
    Topics: Process Engineering, Biotechnology, Nutrition Technology
    Notes: Summary Eggs of the nematode,Necator americanus, were incubated at 28°C for up to 120h in medium (prepared from mouse faeces) supplemented with 0.4% (w/v) or 5% of either commercial grade or a silica-Amberlite purified fraction of the non-ionic surfactant, Pluronic F-68. Exposure to commercial grade or purified Pluronic at both concentrations delayed egg hatching. However, subsequent larval growth, as assessed by length of larva, buccal capsule, oesophagus and tail, was significantly (P〈0.05) increased (maximally following exposure to 5.0% of the purified fraction). No further development occurred in larvae exposed to other Pluronic fractions tested after 48h, irrespective of concentration. These results suggest that Pluronic may be a valuable supplement in media used for the axenic culture of nematode larvae.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01033417
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  • 7
    Electronic Resource
    Electronic Resource
    Effects of Pluronic F-68 on callus growth and protoplast plating efficiency of Solanum dulcamara (1991)
    Springer
    Plant cell reports 10 (1991), S. 52-54 
    Details
    ISSN: 1432-203X
    Keywords: Pluronic F-68 ; Callus growth ; Protoplasts ; Solanum dulcamara
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary The effects of the non-ionic surfactant, Pluronic F-68, on the growth of callus and protoplasts from Solanum dulcamara L. have been studied. Growth of callus was stimulated by addition of 0.1% (w/v) commercial grade Pluronic to culture medium, whereas lower concentrations (0.01% w/v) had no corresponding effect. In contrast, higher concentrations (1.0% w/v) of Pluronic inhibited callus growth. The mean plating efficiency of protoplasts grown at different densities (15 days after plating) was increased up to 26% following culture with 0.1% (w/v) Pluronic, while 0.01% (w/v) Pluronic was ineffective. Mean protoplast plating efficiency decreased by up to 32% following culture with 1.0% (w/v) Pluronic.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00233033
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  • 8
    Electronic Resource
    Electronic Resource
    Stimulation of shoot regeneration from jute cotyledons cultured with non-ionic surfactants and relationship to physico-chemical properties (1993)
    Springer
    Plant cell reports 13 (1993), S. 49-53 
    Details
    ISSN: 1432-203X
    Keywords: Corchorus capsularis ; jute ; cotyledons ; surfactants ; Pluronic F-68 ; Triton X-100 ; Tween 20 ; shoot regeneration
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary The effects have been studied of the non-ionic surfactants, Plutonic F-68, Tween 20 or Triton X-100, on shoot regeneration from cultured jute (Corchorus capsularis L.) cotyledons with attached petioles. This group of non-ionic surfactants was selected in order to determine a possible relationship between the physico-chemical properties of individual compounds and their observable effects on plant morphogenesis in cultured jute cotyledons. Supplementation of culture medium with 0.001–0.5% (w/v) Pluronic F-68 increased the mean percentage of cotyledons producing shoots and the mean number of shoots/cotyledon, with maximal responses at 0.5% (w/v). By contrast, Tween 20 produced maximal effects at 0.001% (v/v), with inhibition of shoot formation at 0.5% (v/v). In both cases, phenotypically normal plants were recovered which could be grown to maturity. Culture of cotyledons with 0.001% (v/v) Triton X-100 similarly increased both the percentage of cotyledons producing shoots and the number of shoots/cotyledon. However, these shoots did not develop into mature plants. Additionally, shoots did not regenerate from cotyledons cultured with Triton at 0.01–0.5% (v/v). These results demonstrate mat there is an apparent relationship between the hydrophilic-hydrophobic (HLB) balance of surfactants which determine their cell permeabilising properties and consequently, their effects on morphogenesis.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00232315
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  • 9
    Electronic Resource
    Electronic Resource
    Synergistic enhancement of protoplast growth by oxygenated perfluorocarbon and Pluronic F-68 (1994)
    Springer
    Plant cell reports 13 (1994), S. 251-255 
    Details
    ISSN: 1432-203X
    Keywords: Petunia hybrida ; protoplasts ; oxygen delivery ; perfluorochemicals ; Pluronic F-68 ; surfactant ; cell division
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Cell suspension-derived protoplasts of albino Petunia hybrida were grown for 10 d at the interface between aqueous culture medium (KM8P) and an oxygenated (10 mbar for 15 min) perfluorocarbon liquid, perfluorodecalin. Protoplasts synthesised new cell walls and divided normally at the perfluorodecalin/culture medium interface, with a mean viability after 10 d of 〉 92.0%. The mean plating efficiency of protoplasts was elevated by 37% (P〈0.05) following culture at the perfluorodecalin/medium interface, but was unaltered by perfluorodecalin or oxygen separately. The mean plating efficiency of protoplasts cultured at the interface was further increased to a maximium of 52% above control, in the presence of oxygenated perfluorodecalin and KM8P medium supplemented with the non-ionic, co-polymer surfactant, Pluronic F-68 at 0.01% (w/v). These findings demonstrate the effectiveness of oxygenated perfluorodecalin for promoting protoplast growth, by facilitating oxygen delivery. The finding that Pluronic F-68 further increased the plating efficiency of protoplasts cultured at the perfluorocarbon/aqueous interface suggests that these agents improve growth through separate, but cumulative, mechanisms.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00233314
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  • 10
    Electronic Resource
    Electronic Resource
    Strategies for promoting division of cultured plant protoplasts: synergistic beneficial effects of haemoglobin (Erythrogen) and Pluronic F-68 (1997)
    Springer
    Plant cell reports 17 (1997), S. 13-16 
    Details
    ISSN: 1432-203X
    Keywords: Key wordsPetunia hybrida ; Oxygen delivery ; Erythrogen ; Haemoglobin ; Pluronic F-68
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Novel approaches, involving supplementation of aqueous culture medium with haemoglobin solution (Erythrogen), in the presence or absence of the copolymer surfactant, Pluronic F-68, have been evaluated to facilitate cellular oxygen availability to promote mitotic division. Cell-suspension-derived protoplasts of albino Petunia hybrida cv. Comanche were cultured for up to 45 days in KM8P medium containing 1:50–1:500 (vol:vol) Erythrogen. The mean initial protoplast plating efficiency after 9 days with 1:50 Erythrogen (18.5%) was significantly greater (P〈0.05) than in untreated controls (11.3%). Supplementation of culture medium with 1:50 Erythrogen, together with 0.01% (wt/vol) Pluronic F-68, increased the mean plating efficiency after 9 days (24.4%) by 92% (P〈0.05) over the control (12.7%). These treatments also produced increases in biomass of protoplast-derived cells up to 2.5-fold greater than control (P〈0.01) over 80 days. Gassing the medium, containing 1:50 Erythrogen, with carbon monoxide abolished the increase in plating efficiency. There was no additional benefit of gassing Erythrogen-supplemented medium with 100% oxygen. The synergistic, beneficial effect of Erythrogen and Pluronic F-68 on protoplast division has implications for plant biotechnology utilising protoplasts.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s002990050343
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