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  • Dendrite Ion channel Na+-K+-ATPase SBFI Sodium Spines Two-photon imaging  (1)
  • NMDA  (1)
  • 1
    Electronic Resource
    Electronic Resource
    Voltage sensitivity of NMDA-receptor mediated postsynaptic currents (1990)
    Springer
    Experimental brain research 81 (1990), S. 209-212 
    Details
    ISSN: 1432-1106
    Keywords: NMDA ; Excitatory postsynaptic current ; Voltage sensitivity ; Patch clamp ; Thin hippocampal slice ; Rat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary Patch-clamp techniques were used to record pharmacologically-isolated N-methyl-D-aspartate-mediated excitatory postsynaptic currents (NMDA-EPSCs) from dentate granule cells in thin rat hippocampal slices. Membrane voltage modulated these EPSCs in two ways. Firstly, depolarization from resting potential enhanced EPSC amplitudes, as expected for a voltage-dependent block by Mg2+ of synaptically activated NMDA receptor channels. Secondly, depolarization markedly prolonged the time course of decay of NMDA-EPSCs in normal and low extracellular Mg2+. Both mechanisms were complementary in establishing a strong dependence between membrane potential and the amount of charge, namely Ca2+, transferred through synaptically activated NMDA receptor channels, that presumably underlies induction of long-term potentiation in the hippocampus.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00230117
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    Paper (German National Licenses)
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  • 2
    Electronic Resource
    Electronic Resource
    Two-photon Na+ imaging in spines and fine dendrites of central neurons (1999)
    Springer
    Pflügers Archiv 439 (1999), S. 201-207 
    Details
    ISSN: 1432-2013
    Keywords: Dendrite Ion channel Na+-K+-ATPase SBFI Sodium Spines Two-photon imaging
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract. Dendritic spines are assumed to be the smallest units of neuronal integration. Because of their miniature size, however, many of their functional properties are still unclear. New insights in spine physiology have been provided by two-photon laser-scanning microscopy which allows fluorescence imaging with high spatial resolution and minimal photodamage. For example, two-photon imaging has been employed successfully for the measurement of activity-induced calcium transients in individual spines. Here, we describe the first application of two-photon imaging to measure Na+ transients in spines and dendrites of CA1 pyramidal neurons in hippocampal slices. Whole-cell patch-clamped neurons were loaded with the Na+-indicator dye SBFI (sodium-binding benzofuran-isophthalate). In situ calibration of SBFI fluorescence with ionophores enabled the determination of the actual magnitude of the [Na+]i changes. We found that back-propagating action potentials (APs) evoked Na+ transients throughout the proximal part of the dendritic tree and adjacent spines. The action-potential-induced [Na+]i transients reached values of 4 mM for a train of 20 APs and monotonically decayed with a time constant of several seconds. These results represent the first demonstration of activity-induced Na+ accumulation in spines. Our results demonstrate that two-photon Na+ imaging represents a powerful tool for extending our knowledge on Na+ signaling in fine cellular subcompartments.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s004249900123
    Library Location Call Number Volume/Issue/Year Availability
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    Paper (German National Licenses)
    Fulltext
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