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  • 1
    Electronic Resource
    Electronic Resource
    Improved sensitivity of detection with the commercial automated gel electrohoresis (HPGE-1000) apparatus through modification of its optical system (1997)
    Weinheim : Wiley-Blackwell
    Electrophoresis 18 (1997), S. 1951-1954 
    Details
    ISSN: 0173-0835
    Keywords: Fluorescence optics ; HPGE-1000 apparatus ; Signal ; noise ratio ; Mirrored incident light ; Effect of mirror blackening ; Detection sensitivity ; Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: In a representative application to a fluorescently detectable protein of commercial automated gel electrophoresis apparatus (HPGE-1000, LabIntelligence, Belmont, CA) the sensitivity of detection by fluorescence was significantly increased by elimination of the mirror below the gel tray. That increase in detection sensitivity is due to a decrease of fluorescent background noise by nearly one order of magnitude, overcompensating a decrease in signal by a factor of two. The resulting increase in signal/noise ratio, i.e., detection sensitivity, should allow for lowered sample loads by which the band width is reduced with benefits to resolution.
    Additional Material: 2 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/elps.1150181112
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    Paper (German National Licenses)
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  • 2
    Electronic Resource
    Electronic Resource
    Enhanced detection sensitivity of “fluorescence reduction” by shifting the analyte absorbance spectrum and use of a fluorescent paper with higher signal/noise ratio (1998)
    Weinheim : Wiley-Blackwell
    Electrophoresis 19 (1998), S. 206-211 
    Details
    ISSN: 0173-0835
    Keywords: Detection sensitivity ; Fluorescence ; Fluorescent paper ; Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: Nonfluorescing protein bands can be detected by the fluorescence optics of the commerical gel electrophoresis apparatus with automated scanning of the migration path (HPGE-1000, LabIntelligence, Belmont CA), taking advantage of the decrease of emission from a fluorescent paper placed below the gel by the absorbance of proteins (“fluorescence reduction”). That decrease of fluorescence gives rise to an inverted protein peak. Nonfluorescent colorless proteins appear to reduce the intensity of light emitted from the fluorescent paper due to absorbance of incident and emitted light. When the absorbance spectrum only slightly overlaps with the excitation and emission spectra of the fluorescent paper, that reduction is weak, and detection sensitivity in that application is consequently only 1/30 of that of fluorescent proteins. By contrast, when the protein is colored so that its absorbance spectrum overlaps widely with the excitation and emission spectra of the fluorescent paper, the sensitivity of “fluorescence reduction” equals 1/4 to 1/5 of that obtained for fluorescent proteins. Bands detected by “fluorescence reduction” provide a quantitative measure of protein load and mobility. The area of the inverted bands is proportional to protein loads up to 16 μg/lane of the gel tray. A theory of “fluorescence reduction” is presented which accounts for the existence of a linear relationship between band area and load.
    Additional Material: 3 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/elps.1150190211
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
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