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  • 1
    ISSN: 1432-2242
    Keywords: Hexaploid wheat ; RFLPs ; Homoeology ; Chromosome arm ; Alien additions
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary To investigate the use of RFLP analysis in the Triticeae, a set of low copy number probes has been isolated from a wheat cDNA library. The probes identify each of the 14 homoeologous chromosome arms of wheat as determined by analysis of DNA fragments hybridizing to the probes in aneuploid lines of Chinese Spring. These probes can be used in RFLP analyses both for the assignment of homoeology of alien chromosomes or arms added to wheat, and for the determination of chromosome dosage in wheat aneuploids. Different chromosomes from various Triticeae species can therefore be followed in a wheat genetic background using a single technique. The potential uses of the set in facilitating the transfer of alien segments into wheat are outlined.
    Type of Medium: Electronic Resource
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  • 2
    Electronic Resource
    Electronic Resource
    Springer
    Theoretical and applied genetics 76 (1988), S. 691-699 
    ISSN: 1432-2242
    Keywords: Hexaploid wheat ; β-Amylase ; Isozymes ; RFLPs ; IEF
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Forty-one hexaploid wheat genotypes have been examined for RFLPs detected by a β-amylase probe using three restriction enzymes, and for mature grain β-amylase isozyme polymorphism following IEF. The two homoeoallelic series assayed for RFLPs differed: little variation was found at group 2 chromosome homoeoloci, while the group 4/5 chromosome homoeoloci displayed considerable variation. Varieties that displayed a RFLP with one RE almost always did likewise with the other two REs, suggesting that most of the polymorphisms observed were due to large DNA rearrangements. Comparison of the variation in grain β-amylase isozymes with the RFLP results indicated strong associations between particular RFLP and isozyme alleles.
    Type of Medium: Electronic Resource
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  • 3
    Electronic Resource
    Electronic Resource
    Springer
    Cell & tissue research 222 (1982), S. 283-298 
    ISSN: 1432-0878
    Keywords: LH-RH-neurones ; Diencephalon ; Immunohistochemistry ; Chicken
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary Nerve fibres and perikarya containing LH-RH-like material were identified immunohistochemically in the diencephalon of the domestic hen using the peroxidase-anti-peroxidase technique. Perikarya were thinly scattered in bilateral bands close to the third ventricle extending from the nucleus praeopticus paraventricularis magnocellularis, passing in front of the anterior commissure into the septal area. In this latter area, the perikarya tended to spread out laterally. A few perikarya were seen in the anterior portion of the nucleus paraventricularis magnocellularis but were not found in the infundibular nuclear complex. Fibre tracts were seen running dorso-ventrally in the preoptic area apparently associated with the lamina terminalis. Fibres, possibly nerve terminals, were found in the lamina terminalis and in the external layers of the anterior and posterior divisions of the median eminence. A large number of fibres was seen distributed throughout the infundibular nuclear complex; scattered fibres were found close to the third ventricle in the anterior hypothalamus. Extrahypothalamic fibres were also observed to project from the septal area into other parts of the telencephalon.
    Type of Medium: Electronic Resource
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