ISSN:
1432-0983
Keywords:
Diethofencarb
;
Benzimidazole resistance
;
β-tubulin
;
Neurospora crassa
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
Notes:
Abstract We have previously shown that increased sensitivity to diethofencarb in the carbendazim(MBC)-resistant F914 strain of Neurospora crassa is caused by a single amino-acid change in β-tubulin, 198Glu to Gly. Three diethofencarb-resistant mutants that are also resistant to MBC were isolated from strain F914. They contained single base-pair-substitution mutations in the β-tubulin gene. The amino acid changes in β-tubulin, Phe from 250Leu, Val from 165Ala, and Ala from 237Thr, were responsible for diethofencarb-resistance in the mutant strains FR511, FR513, and FR421, respectively. The amino acid at position 198 of β-tubulin in these mutants was Gly, which is the same as in strain F914. β-tubulin genes with 198Glu were constructed by site-directed mutagenesis. The altered β-tubulin genes derived from FR511 and FR421 transformed the wild-type strain to resistance to MBC, indicating that 250Phe and 237Ala in β-tubulin are responsible for resistance not only to diethofencarb but also to MBC.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF00351780
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