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  • 1
    Electronic Resource
    Electronic Resource
    Springer
    Cell & tissue research 108 (1970), S. 475-486 
    ISSN: 1432-0878
    Keywords: Leydig cells ; mice ; Differentiation
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary Testicular interstitial cells in ddN mice aged 3 to 28 days were studied with the electron microscope. They differed from those in adult mice in several respects: 1) Agranular endoplasmic reticulum and granular endoplasmic reticulum were mixed in the youngest animals, while they were separated topographically in 28 day mice. 2) Mitochondria were usually round between 3 to 10 days, while oval or rod-shaped mitochondria predominated in 28 day animals. Intramitochondrial granules were absent between 3 to 10 days but were occasionally observed in the older animals. 3) Glycogen particles were occasionally arranged in irregular clusters in 3 day old animals, while these structures were absent in the older animals. 4) Membranous whorls were recognized only in 28 day mice. 5) Villous processes on the cell surface facing the perivascular space were poorly developed in the youngest animals, while in 28 day mice they were as prominent as in the adult mouse.
    Type of Medium: Electronic Resource
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  • 2
    ISSN: 1432-0878
    Keywords: Leydig cell ; Testis ; Interstitium ; Aging ; Ultrastructure ; Morphometry ; Testosterone ; Rat (Wistar)
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Abstract The ultrastructure of testicular interstitium in young and aged adult rats was analysed using morphometric methods, and the plasma testosterone concentration was measured. With increasing age there was an augumentation in the volume of collagen fibrils in the intercellular matrix and in blood vessels. During the aging process (approximately two years) the average volume of the Leydig cell decreased from 1364 μm3 to 637 μm3, but the number of Leydig cells in paired testes increased from 53x106 to 113x106. The absolute volume of smooth surfaced endoplasmic reticulum (SER) per Leydig cell amounted in aged rats to 78% of that in young adult rats. The total amount of SER in paired testes increased by 62% with aging. The present analysis suggests that the ability of SER to maintain peripheral testosterone concentration decreases with age. In young adult rats the absolute volume of peroxisomes per Leydig cell correlated significantly with the concentration of testosterone in blood and also with the absolute volume of SER per Leydig cell. These results combined with ultrastructural observations of close apposition of peroxisomes and SER suggest that peroxisomes have a role in testosterone secretion by Leydig cells.
    Type of Medium: Electronic Resource
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