ISSN:
1432-0878
Keywords:
Macrophage
;
Differentiation
;
Triolein
;
Stereology
;
Electron microscopy
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
,
Medicine
Notes:
Summary The results of an objective two- and three-dimensional analysis of the morphological features of normal and triolein-induced mouse peritoneal macrophages are reported. An “equivalent circle“ technique for resolving the effects of volume and surface area on volume-to-surface parameters is described. The method is a simple comparative one which does not require the actual determination of cell volume. Macrophage stimulation promoted increases in mean cell size, cytoplasmic granularity and volume-to-surface ratio. In addition, a reduction in nuclear volume-to-surface ratio accompanied in vivo stimulation. Nucleocytoplasmic ratio remained constant. The equivalent circle procedure showed that the increase in cellular volume-to-surface ratio was due largely to the increase in cell volume; the decrease in nuclear volume-to-surface ratio was primarily the result of a substantial increase in nuclear membrane surface area. Stereological estimations suggest that interiorized cell membrane (in the form of triolein-containing phagosomes) is replaced by newly reconstructed surface membrane.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF00306694
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