ISSN:
1437-1596
Keywords:
Capillary gas chromatography, opiates
;
Drug screening, determination of capillary gas chromatography
;
Capillargaschromatographie
;
Opiatnachweis
;
Drogenscreening
;
Capillargaschromatographie
Source:
Springer Online Journal Archives 1860-2000
Topics:
Medicine
,
Law
Description / Table of Contents:
Zusammenfassung Es wird eine Capillargaschromatographische Methode zur simultanen Bestimmung von Morphin, Codein, Heroin, 3- und 6-Monoacetylmorphin, Nalorphin, Naloxone, Naltrexon und Ethylmorphin beschrieben. Von 2ml Plasma, Urin oder anderen biologischen Proben (z.B. Gewebepräparate) wurden die Substanzen in alkalischen Milieu durch Chloroform: isopropylalkohol:n-heptan extrahiert, anschließend in Chloroform rückextrahiert. Nach Derivatiesierung mittels Trifluoressigsäureanhydrid wurden die aliquoten Teile an einer 25 m Capillarsäule eingesetzt. Die Detektion erfolgte durch einen Nitrogen-Phosphorous Detektor, und die Nachweisgrenzen und Wiederfindungsraten wurden ebenfalls ermittelt.
Notes:
Summary A capillary column gas chromatographic method is described for the simultaneous determination of morphine, codeine, heroin, 3- and 6-monoacetylmorphine, nalorphine, naloxone, ethylmorphine, and naltrexone. The drugs were extracted from 2ml plasma, urine, or other biological samples, including tissue under alkaline conditions in chloroform-isopropanol-n-heptane (50∶17∶33, v/v), with levallorphan as an internal standard. The drugs were extracted into acid and then reextracted into chloroform after the acid had been alkalinized. After derivatization with trifluoroacetic anhydride, an aliquot was injected into a 25m capillary column equipped with a nitrogen phosphorus detector. The lower limits of detectability, extraction recovery, and the within-run and day-to-day precision of results were determined for each drug. Our results indicate that the procedure is suitable for use in overdose screening and therapeutic drug monitoring.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF01255847
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