ZIB

Hits per page

hits 1 - 2 | 2 hits

Sorting

Electronic Resource

K252a: A new blocker of the cell-cycle at G1 phase in a human hepatoma cell line (1993)

Nakayama, T. ; Hashimoto, Y. ; Kaneko, Y. ; [et al.]

Springer

Cellular and molecular life sciences 49 (1993), S. 876-880

add to mindlist on the mindlist

Details

ISSN: 1420-9071

Keywords: K252a ; G1 block ; cell size ; c-myc ; albumin ; HuH7

Source: Springer Online Journal Archives 1860-2000

Topics: Biology , Medicine

Notes: Abstract The administration of 200 nM K252a to HuH7 suppressed the proliferation of the cells almost completely. The uptake of [3H]thymidine was inhibited, and flow cytometry revealed only one peak at 2C on day 3 after treatment with 100 nM K252a. The expression of proto-oncogene c-myc was not reduced. Despite the blockage at G1, both the size of the cells and the amount of cell protein had increased by 4 times by day 3 after treatment with K252a, while the cells secreted albumin and α-fetoprotein into the medium as usual. These results show that K252a can increase the cell size of HuH7 without losing its function by blocking the cell cycle at G1 phase.

Type of Medium: Electronic Resource

URL: http://dx.doi.org/10.1007/BF01952601

Permalink

Library	Location	Call Number	Volume/Issue/Year	Availability

Others were also interested in ...

Paper (German National Licenses)

Fulltext

Electronic Resource

Numerical simulation and hydrodynamic visualization of transient viscous flow around an oscillating aerofoil (1989)

Daube, O. ; Phuoc, L. Ta ; Dulieu, A. ; [et al.]

Chichester : Wiley-Blackwell

International Journal for Numerical Methods in Fluids 9 (1989), S. 891-920

add to mindlist on the mindlist

Details

ISSN: 0271-2091

Keywords: Transient flows ; Oscillating aerofoil ; Dynamic stall ; Navier-Stokes equations ; Finite differences ; Engineering ; Engineering General

Source: Wiley InterScience Backfile Collection 1832-2000

Topics: Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics

Notes: Unsteady viscous flow around a large-amplitude and high-frequency oscillating aerofoil is examined in this paper by numerical simulation and experimental visualization. The numerical method is based on the combination of a fourth-order Hermitian finite difference scheme for the stream function equation and a classical second-order scheme to solve the vorticity transport equation. Experiments are carried out by a traditional visualization method using solid tracers suspended in water. The comparison between numerical and experimental results is found to be satisfactory. Time evolutions of the flow structure are presented for Reynolds numbers of 3 × 103 and 104. The influence of the amplitude and frequency of the oscillating motion on the dynamic stall is analysed.

Additional Material: 10 Ill.