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  • 1
    Electronic Resource
    Electronic Resource
    Circadian rhythm in type I collagen formation in postmenopausal women with and without osteopenia (1995)
    Springer
    Osteoporosis international 5 (1995), S. 472-477 
    Details
    ISSN: 1433-2965
    Keywords: Circadian rhythm ; Cosinor analysis ; Osteopenia ; Postmenopause ; Procollagen type I carboxyl-terminal propeptide (PICP)
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract A circadian rhythm in the serum concentration of the procollagen type I carboxyl-terminal propeptide (sPICP) has previously been demonstrated in premenopausal women. This study was performed to investigate the circadian rhythm in sPICP in healthy and osteopenic postmenopausal women. Blood samples were taken every third hour for 27 h from three groups of women: 12 early postmenopausal women (aged 55±2 years; mean±SD); 12 late postmenopausal women (aged 73±1 years); and 12 osteopenic but otherwise healthy late postmenopausal women (aged 73±1 years). A circadian rhythm in sPICP was found in all three groups, as shown by cosinor analysis (p=0.000003−0.03). The circadian rhythm in sPICP was significantly different between the osteopenic group and the age-matched healthy group (p〈0.008). The amplitude of the circadian rhythm in sPICP was about twice as high in the osteopenic group, and the time of the maximum tended to be about 3 h later, as compared with the age-matched healthy group. The plasma concentration of osteocalcin, as measured by a recently developed two-site enzyme-linked immunosorbent assay, also showed a circadian rhythm in all three groups (p=0.0001−0.05), with no significant differences between groups. In conclusion, we have found a significant circadian rhythm in sPICP in both early and late postmenopausal women. In osteopenic women the nightly peak in sPICP is larger and persists later into the night as compared with non-osteopenic women.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01626611
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  • 2
    Electronic Resource
    Electronic Resource
    Enzyme-linked immunosorbent assay of urokinase-type plasminogen activator (uPA) in cytosolic extracts of human breast cancer tissue (1993)
    Springer
    Breast cancer research and treatment 28 (1993), S. 223-229 
    Details
    ISSN: 1573-7217
    Keywords: breast cancer ; cytosols ; ELISA ; urokinase-type plasminogen activator
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary The enzyme urokinase-type plasminogen activator (uPA) plays a role in cancer invasion, and high levels of uPA in detergent extracts of mammary cancer tissue have been reported to be associated with a poor prognosis. We have explored the possibility of using mammary cancer cytosol extracts routinely prepared for steroid receptor analysis for retrospective prognostic studies of uPA. A sandwich enzyme-linked immunosorbent assay (ELISA) for uPA was developed, using polyclonal catching antibodies and a mixture of three biotinylated monoclonal detecting antibodies, that were selected to recognize free uPA, inhibitor-bound uPA, and uPA bound to its cell surface receptor. The assay detects active uPA and its inactive proenzyme form, pro-uPA, equally well. The limit of detection is approximately 1 pg of pro-uPA in a volume of 100 µl, and there is a linear dose-response up to 100 pg pro-uPA. The efficiency in extracting uPA of a neutral non-detergent buffer used to prepare cytosol extracts was compared with that of 4 other buffers. There was a pronounced difference in the efficiency, the most efficient being a pH 4.2 buffer containing the non-ionic detergent Triton X-100, while the least efficient was the buffer used to prepare cytosols. Nevertheless, uPA immunoreactivity was readily measurable in the cytosols, and there was a close correlation between the amounts of uPA extracted under optimal conditions and those routinely used for steroid hormone receptor analysis. While the amount of uPA extracted under the latter conditions constituted only about 12% of that optimally extractable, we conclude that the ELISA described herein can be used to retrospectively analyze the potential prognostic value of uPA-concentrations in cytosols prepared for analysis of steroid hormone receptors.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00666583
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    Paper (German National Licenses)
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