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  • 1
    ISSN: 1432-203X
    Keywords: Key words Spinach (Spinacia oleracea L.) ; Regeneration ; Green-fluorescent protein ; Transformation ; Cotyledon
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract An efficient transformation and regeneration system was established for the production of transgenic spinach (Spinacia oleracea L.) plants. Cotyledon explants were infected with Agrobacterium tumefaciens strain LBA4404 carrying the selectable marker gene, neomycin phosphotransferase II (nptII), and the reporter gene smgfp, encoding soluble-modified green-fluorescent protein, driven by the cauliflower mosaic virus 35S promoter. The infected explants were cultured on Murashige and Skoog medium, containing 1 mg/l benzyladenine and 0.4 mg/l naphthaleneacetic acid. Shoots were regenerated on selection medium containing 50 mg/l kanamycin. Regenerated kanamycin-resistant shoots were rooted on medium containing 1 mg/l indolebutyric acid and subsequently grown in soil in the greenhouse. Southern blot analysis indicated that the smgfp gene had been integrated into the spinach genome. Northern and Western blots showed that the smgfp gene was expressed in progeny plants.
    Type of Medium: Electronic Resource
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  • 2
    Electronic Resource
    Electronic Resource
    Chichester [u.a.] : Wiley-Blackwell
    International Journal of Numerical Modelling: Electronic Networks, Devices and Fields 9 (1996), S. 215-223 
    ISSN: 0894-3370
    Keywords: Engineering ; Electrical and Electronics Engineering
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Electrical Engineering, Measurement and Control Technology
    Notes: A time domain unconditionally stable explicit method is presented which may be used to model processes governed by the diffusion equation such as transient heat flow. Unlike the explicit finite difference routine, the method models the diffusion process within the time step (MPWT). Being explicit, the method (MPWT) may easily account for non-linear physical parmeters and may be compared with the transmission line matrix (TLM) method of diffusion modelling in terms of its utility. Unlike the TLM model, however, the equivalent circuit does not contain inductive components which have no clear physical meaning. The MPWT method is based on a simple resistive and capacitive circuit model, which for short time steps reduces to the explicit finite difference formulation but is stable for large time steps. Good agreement has been found when results obtained using this algorithm are compared with other numerical methods and analytical results.
    Additional Material: 7 Ill.
    Type of Medium: Electronic Resource
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