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  • 1
    Electronic Resource
    Electronic Resource
    Interspecific hybrid plant formation by electrofusion in Nicotiana (1987)
    Springer
    Theoretical and applied genetics 75 (1987), S. 1-4 
    Details
    ISSN: 1432-2242
    Keywords: Electrofusion ; Fertile somatic hybrid ; Fraction I protein analysis ; Nicotiana
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary We obtained complete hybrid plants by electrofusion of mesophyll protoplasts from Nicotiana glauca and N. langsdorffii. Electrofocusing analysis of Fraction I proteins isolated from the leaves of these plants confirmed their hybridity. Cytological analysis indicated that the chromosome number (2n) of these plants is between 60 to 66, suggesting that they are the products of triple fusion. All plants were fertile and set viable seeds after self pollination. As we did not use an AC field for electrofusion, the present results indicate that an AC field is not essential for obtaining hybrid plants with electrofusion.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00249133
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    Paper (German National Licenses)
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  • 2
    Electronic Resource
    Electronic Resource
    Fluorescent banding pattern analysis of eight taxa of Phaseolus and Vigna in relation to their phylogenetic relationships (1993)
    Springer
    Theoretical and applied genetics 87 (1993), S. 38-43 
    Details
    ISSN: 1432-2242
    Keywords: Fluorescent chromosome banding patterns ; Phaseolus ; Vigna ; Phylogenetic relationships
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Phylogenetic relationships among eight taxa of seven species of Phaseolus and Vigna (Phaseolus angularis, P. aureus, P. calcaratus, P. coccineus, P. vulgaris, Vigna sesquipedalis and V. sinensis; 2n = 22 each) were studied by the fluorescent chromosome banding technique. Preparations of somatic metaphase chromosomes of each taxon were sequentially stained with Giemsa, GC-specific fluorochrome chromomycin A3 (CMA) and AT-specific fluorochrome 4′-6-diamidino-2-phenylindole (DAPI). On the basis of the fluorescent banding patterns of the 22 chromosomes of each taxon, P. angularis, P. coccineus (from China and Korea) and P. vulgaris were grouped into one group (“Phaseolus group”), P. aureus and two Vigna species were grouped into another (“Vigna group”) and P. calcaratus was grouped in an independent group.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00223741
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    Paper (German National Licenses)
    Fulltext
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