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  • Electrophoresis  (2)
  • Erysiphe graminis f. sp.tritici  (1)
  • 1
    Electronic Resource
    Electronic Resource
    A computer-assisted examination of the storage protein genetic variation in 841 accessions of Triticum dicoccoides (1984)
    Springer
    Theoretical and applied genetics 69 (1984), S. 79-86 
    Details
    ISSN: 1432-2242
    Keywords: Electrophoresis ; Endosperm ; Proteins ; Emmer ; Wheat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Triticum turgidum L. var. dicoccoides (wild emmer) is an important genetic resource for increasing the protein content of common wheat (Triticum aestivum L.). Many studies have shown that the presence or absence of bands in sodium dodecyl sulfate polyacrylamide (SDS-PAGE) electrophoregrams of wheat storage proteins to be of a purely genetic character. A total protein extraction and SDS-PAGE technique was used to estimate the storage protein genetic variability among 841 accessions of wild emmer collected from various ecological regions in the Middle East. In addition, a computer data bank was developed, recording the onedimension electrophoregram bands for each accession by molecular weight (MW) and relative Coomassie Blue staining intensity as determined from densitometer scans. Analyses of this information are being used to identify specific accessions for further study by two dimension electrofocusing-electrophoresis and breeding and genetic analyses. The computer-assisted analyses indicated that the greatest genetic variability occurs for proteins in the high MW region (above 70,000 MW) followed by those in the medium range (70,000 to 33,300 MW). Comparatively little variability was revealed for protein subunits of below 33,300 MW.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00262545
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    Paper (German National Licenses)
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  • 2
    Electronic Resource
    Electronic Resource
    Quantitative variation in the kernel proteins among 841 accessions of Triticum dicoccoides estimated by SDS-PAGE (1986)
    Springer
    Theoretical and applied genetics 72 (1986), S. 296-301 
    Details
    ISSN: 1432-2242
    Keywords: Electrophoresis ; Endosperm ; Proteins ; Wild emmer ; Wheat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary The relative proportion and amount of proteins in five defined molecular weight (MW) regions (A1=above 71,000=71K, A2=71K−49K, A3=49K−31K, A4=31K−20K, A5=20K and less) were estimated by densitometric analyses of the amount of dye bound by kernel proteins (Fullington et al. 1980) of Triticum dicoccoides SDS-PAGE gels. These MW regions roughly correspond to the wheat protein solubility classes (Cole et al. 1981; Fullington et al. 1983). One purpose of the study was to select accessions whose seed proteins bind relatively high amounts of dye in the glutenin and albumin globulin regions. These accessions will be used for further in-depth studies as possible candidate donors of genes to improve the baking and nutritional quality of wheat. Marked differences in the quantitative relationships were found among the proteins in the five MW regions. Coefficients of variation (CV's) for the highest peak (i.e., most abundant protein) MW in different protein MW regions were similar for A1, A2 and A3, at 11.4, 11.7, and 11.1%, respectively, but only 4.1 for A4, and 10.6% for region A5. The CV for the highest peak MW overall was 29.8. Accession BP0649, for example, had over 44% of its protein in region A5, whereas BP0566 (lowest among the top 10%) had only 21.4% of its protein in that region. Over 37% of the proteins of accessions BP0649 and 0001 to 0005 was in region A5. At least 84 accessions with the highest amount of protein in region A5, and 13 accessions with more protein in region A1 than Chinese Spring may merit further evaluation as possible protein gene donors. High amounts of protein in A1 may be of importance in bread-baking quality, and in A4 and A5 for high lysine wheat. Accessions in both extremes were selected to test these hypotheses. All accessions are now or will be available in the USDA Wheat Collection.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00288564
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  • 3
    Electronic Resource
    Electronic Resource
    A comparative study of resistance to powdery mildew in wild emmer wheat in the seedling and adult plant stage (1989)
    Springer
    European journal of plant pathology 94 (1989), S. 177-184 
    Details
    ISSN: 1573-8469
    Keywords: Erysiphe graminis f. sp.tritici ; Triticum dicoccoides
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Description / Table of Contents: Samenvatting Van 272 wilde emmer (Triticum dicoccoides) herkomsten werd de resistentie tegen meeldauw (Erysiphe graminis f.sp.tritici) bepaald. Waarnemingen werden gedaan in het kiemplantstadium en tweemaal in het volwassenplantstadium. Voor de evaluatie van de resistentie in het volwassenplantstadium werden twee methoden vergeleken. Bij de ene methode werd gelet op mate van chlorose en puistgrootte, terwijl bij de tweede methode het hoogste bladniveau werd bepaald waarop meeldauwpuistjes werden waargenomen. Met de eerste methode bleken 133 herkomsten zowel in het kiemplantstadium als in het volwassenplantstadium resistent te zijn, terwijl met de tweede methode 134 herkomsten in het kiemplantstadium resistent waren en in het volwassenplantstadium geen sporulatie vertoonden of slechts op de onderste bladeren. Bij vergelijking van de kiemplantreactie met de reactie in het volwassenplantstadium kon onderscheid gemaakt worden tussen resistentie die alleen werkzaam is in het kiemplantstadium (echte kiemplantresistentie), resistentie die alleen in het volwassenplantstadium werkt (volwassenplantresistentie) en resistentie die in alle groeistadia bescherming geeft (‘overall’ resistentie). Binnen de groep van 26 herkomsten, die zowel in het volwassenplantstadium als in het kiemplantstadium een vatbaar infectietype vertoonden, bleken 6 herkomsten een lage aantastingsgraad te hebben. Dit duidt op het voorkomen van partiële resistentie. Concluderend kan gesteld worden dat resistentie tegen meeldauw veelvuldig voorkomt in herkomsten van wilde emmer tarwe en dat deze resistentie wordt veroorzaakt door genen met verschillende werkingsmechanismen.
    Notes: Abstract In a comparative study of 272 entries of wild emmer (Triticum dicoccoides), resistance to powdery mildew (Erysiphe graminis f. sp.tritici) was determined both in the seedling and the adult plant stage. The performance of the entries was evaluated with two methods, scoring of infection types and noting the uppermost leaf which had become affected. With the first method, 133 entries were resistant both in the seedling stage and the adult plant stage, while with the second method 134 entries were resistant in the seedling stage and free of infection or affected only on the lower leaves in the adult plant stage. Moreover, 26 entries which were susceptible in the seedling stage, became moderately resistant or resistant in the adult plant stage. In general, there was good agreement between seedling and adult plant reactions. Screening for resistance to powdery mildew in the seedling stage was shown to be a reliable method to secure selections for wheat breeding programmes. By screening in the adult plant stage and comparing with the seedling reaction, four different types of resistance were found, namely true seedling resistance, overall resistance, adult plant resistance and partial resistance.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02006543
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    Paper (German National Licenses)
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