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  • 1
    Electronic Resource
    Electronic Resource
    Polymorphisms in mitochondrial DNA of european and Africanized honeybees (Apis mellifera) (1988)
    Springer
    Cellular and molecular life sciences 44 (1988), S. 257-260 
    Details
    ISSN: 1420-9071
    Keywords: Mitochondrial DNA ; restriction site polymorphisms ; Apis mellifera ; Africanized honey bees
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary This study demonstrates polymorphisms in both the length and in the restriction enzyme cleavage sites of honeybee mitochondrial DNA (mtDNA). The levels of variation are typical of those found in other metazoan species. These polymorphisms are potentially useful for the identification of Africanized bees in the western hemisphere and for study of honeybee phylogenetics.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01941730
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    Paper (German National Licenses)
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  • 2
    Electronic Resource
    Electronic Resource
    Stable transformation and regeneration of transgenic plants of Pinus radiata D. Don (1998)
    Springer
    Plant cell reports 17 (1998), S. 460-468 
    Details
    ISSN: 1432-203X
    Keywords: Key wordsPinus radiata ; Transformation ; Biolistic ; Embryogenic tissue
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract A biolistic particle delivery system was used to genetically transform embryogenic tissue of Pinus radiata. The introduced DNA contained a uidA reporter gene under the control of either the tandem CaMV 35S or the artificial Emu promoter, and the npt II selectable marker controlled by the CaMV 35S promoter. The average number of stable, geneticin-resistant lines recovered was 0.5 per 200 mg fresh weight bombarded tissue. Expression of the uidA reporter gene was detected histochemically and fluorimetrically in transformed embryogenic tissue and in derived mature somatic embryos and regenerated plants. The integration of uidA and npt II genes into the Pinus radiata genome was demonstrated using PCR amplification of the inserts and Southern hybridisation analysis. The expression of both genes in transformed tissue was confirmed by Northern hybridisation analysis. More than 150 transgenic Pinus radiata plants were produced from 20 independent transformation experiments with four different embryogenic clones.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s002990050426
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    Paper (German National Licenses)
    Fulltext
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