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Rapid detection of hemoglobin variants by mutagenically separate polymerase chain reaction (MS-PCR) (1995)

Chang, J. G. ; Chang, C. P. ; Lu, C. M. ; [et al.]

Springer

Annals of hematology 71 (1995), S. 97-100

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ISSN: 1432-0584

Keywords: Hemoglobin variant ; MS-PCR ; Allele-specific primer

Source: Springer Online Journal Archives 1860-2000

Topics: Medicine

Notes: Abstract The detection of molecular defects of hemoglobin variants using mutagenically separated polymerase chain reaction (MS-PCR) was applied in this study. Using different lengths of allele-specific mutagenic primers, normal and mutant alleles of hemoglobin genes were amplified in the same reaction tube. Subsequent gel electrophoresis showed at least one of the two allelic products at the same loci or at least two of the several allelic products at different loci. We employed MS-PCR to test the following hemoglobin variants: Hb Constant Spring (Hb CS), Hb E, Hb G-Taichung, Hb J-Meinung, and Hb Kaohsiung. The results were the same as those obtained by amplified created reaction sites (ACRS) or direct sequencing. We conclude that the MS-PCR provides a rapid and simple alternative to other techniques for mutation detection in hemoglobin variants. Moreover, the principle can be extended to other genetic diseases.

Type of Medium: Electronic Resource

URL: http://dx.doi.org/10.1007/BF01699253

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Modal reaction method for modal participation factors in support motion problems (1995)

Chen, J. T. ; Hong, H.-K. ; Yeh, C. S.

Chichester : Wiley-Blackwell

Communications in Numerical Methods in Engineering 11 (1995), S. 479-490

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ISSN: 1069-8299

Keywords: Modal reaction method ; modal participation factor ; support motion ; discrete system ; modal equivalent mass ; quasi-static decomposition ; Engineering ; Engineering General

Source: Wiley InterScience Backfile Collection 1832-2000

Topics: Mathematics , Technology

Notes: An efficient method, called the modal reaction method, for calculating the modal participation factors for support motion problems is proposed. It is found that modal reactions at supports proportionally describe the magnitudes of the modal participation factors. Thus, the modal participation factor for support motion problems can be calculated very efficiently, saving more than 99 per cent, for the given example with 640 degreees of freedom, of the CPU time compared with using the popular quasistatic decomposition method.

Additional Material: 6 Ill.