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  • 1
    Electronic Resource
    Electronic Resource
    Synthesis of the peptide-transport carrier of barley scutellum during the early stages of germination (1985)
    Springer
    Planta 164 (1985), S. 550-556 
    Details
    ISSN: 1432-2048
    Keywords: Germination (seed) ; Hordeum (peptide transport) ; Peptide transport ; Protein synthesis ; Scutellum ; Seed germination ; Thiol group
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Development of peptide-transport activity in the scutella of isolated barley (Hordeum vulgare l. cv. Maris Otter, Winter) embryos is shown to increase rapidly after about 15 h of imbibition, with the bulk of the transport activity being expressed by 24 h. This development is prevented by treatment of 15 h embryos with cycloheximide. Protein synthesis is found to increase in a closely related manner and also to be abolished by cycloheximide. Measurement of the incorporation of bound [35S]methionine by 15 to 21-h embryos indicates that de-novo protein synthesis during this period is greater in the scutellum than in the embryonic axis. Previous studies have shown that the peptide-transport system possesses essential dithiol groups, probably located at the substrate-binding site (Walker-Smith and Payne 1983 b, 1984b). Treatment of 15-h embryos with the non-penetrant thiol reagent p-chloromercuribenzene sulphonic acid did not affect development of peptide-transport activity during the following 6 h, whereas with 3-d embryos identical treatment inhibited uptake almost completely during a subsequent 6-h period. Radioautography revealed that amongst the proteins synthesised during this early phase of germination and labelled in vitro with [35S]methionine some are found within the epithelial plasmalemmae of the scutellum, which is the location of the peptide-transport carrier identified previously by externally labelling with a radioactive thiol reagent. The results provide evidence that protein(s) of the peptide-transport system are synthesised and inserted into the scutellum during early germination, allowing the system to play a major role in the nitrogen nutrition of the embryo.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00395974
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  • 2
    Electronic Resource
    Electronic Resource
    Characteristics of the protein carrier of the peptide-transport system in the scutellum of germinating barley embryos (1984)
    Springer
    Planta 162 (1984), S. 166-173 
    Details
    ISSN: 1432-2048
    Keywords: N-Ethylmalemide ; Germination (seed) ; Hordeum (embryo, peptide) ; Peptide transport ; Thiol group ; Transport carrier protein
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Through the use of the protein reagents N-ethylmaleimide, p-chloromercuribenzenesulphonic acid and phenylarsine oxide, it is shown that in the scutellum of the germinating barley embryo, the transport of peptides, but not the transport of amino acids or glucose is specifically thiol-dependent. Furthermore, these essential thiol groups are shown to exist as redox-sensitive, vicinal-dithiols that lie at the substrate-binding sites of the peptide-transport proteins. The binding of N-ethylmaleimide to these dithiols is shown to be very fast, matching the kinetics of inhibition of peptide transport by this reagent. A technique for the specific labelling of the dithiols with N-ethyl[2,3-14C]maleimide is described, which allows the carrier proteins to be visualized at the scutellar epithelium using radioautography and permits calculation of the approximate amount of peptide-transport protein present per scutellum. In related studies, the importance of arginyl and histidyl residues to both amino-acid and peptide transport is shown, although other residues, e.g. carboxyl ligands do not seem to be critically involved.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00410214
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  • 3
    Electronic Resource
    Electronic Resource
    Characteristics of the active transport of peptides and amino acids by germinating barley embryos (1984)
    Springer
    Planta 162 (1984), S. 159-165 
    Details
    ISSN: 1432-2048
    Keywords: Amino acid transport ; Germination (seed) ; Hordeum (peptide) ; Peptide transport ; Scutellum ; Storage protein mobilization
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Use of two different assays involving either radioactively labelled substrates or a fluorescent-labelling procedure, gave good agreement for the rates of transport of peptides and amino acids into the scutellum of germinating grains of barley (Hordeum vulgare cv. Maris Otter, Winter). However, evidence was obtained for the enzymic decarboxylation of transpored substrate, which can cause underestimates of transport rates when using radioactively labelled substrates. The peptide Gly-Phe, was shown to be rapidly hydrolysed after uptake, and autoradiography of transported Gly-[U-14C]Phe indicated a rapid distribution of tracer, i.e. [U-14C] phenylalanine into the epithelium and sub-epithelial layers of the scutellum. The developmental patterns of transport activity indicate that peptide transport is more important nutritionally during the early stages of germination (1–3 d) whereas amino acids become relatively more important later (4–6 d). A range of amino acids is shown to be actively transported and several compete for uptake. At physiological concentrations, e.g. 2mM, transport of peptides and amino acids is inhibited about 80% by protonophore uncouplers, but at higher concentrations (10–100 mM) passive uptake predominates.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00410213
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  • 4
    Electronic Resource
    Electronic Resource
    Microbial diversity: ex situ conservation of Indonesian microorganisms (1997)
    Springer
    World journal of microbiology and biotechnology 13 (1997), S. 359-361 
    Details
    ISSN: 1573-0972
    Keywords: Biodiversity ; conservation ; fermented foods ; fungi ; Indonesia
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Abstract This paper discusses the role of microbial resource collections in the ex situ conservation of microbial diversity and, in particular, the activities of a recently completed Darwin Initiative project to facilitate collection, identification and exploitation of Indonesian microbial diversity. This helped revitalize the collection of the Lembaga Ilmu Pengetahuea Indonesia (Indonesian Institute of Sciences), LIPI, Research and Development Centre for Biology, Bogor, Indonesia. It is suggested that a focus on microorganisms beneficial to man is essential to enable ex situ collections to be a useful tool in the conservation and sustainable utilization of microbial diversity. This task is enormous and far beyond the capabilities of one collection and its achievement will necessitate a network of collections.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1023/A:1018559729803
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  • 5
    Electronic Resource
    Electronic Resource
    Recovery of fungi after storage for over a quarter of a century (1999)
    Springer
    World journal of microbiology and biotechnology 15 (1999), S. 517-519 
    Details
    ISSN: 1573-0972
    Keywords: Cryopreservation ; freeze-drying ; fungi ; oil storage ; silica gel
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Abstract This paper discusses the recovery of a sample of strains originally preserved on silica gel over the period 1970–1973. Fifty-three strains were tested of which 18 recovered, demonstrating survival for more than 20 years. The recovery of 26 of the strains is directly compared with that of replicates from oil storage and freeze-drying. A summary of storage of 421 strains by the silica gel technique is given, reporting survival of 64% for a quarter of a century or more. The technique is ideal for preserving sporulating fungi of the Ascomycota and many species of mitotic fungi for laboratories of limited facilities.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1023/A:1008959521962
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  • 6
    Electronic Resource
    Electronic Resource
    Cryogenic light microscopy and the development of cooling protocols for the cryopreservation of filamentous fungi (1997)
    Springer
    World journal of microbiology and biotechnology 14 (1997), S. 49-57 
    Details
    ISSN: 1573-0972
    Keywords: Cooling rate ; cryogenic light microscopy ; cryopreservation ; exosmosis ; fungi
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Abstract One hundred and ninety five strains of fungi were observed during freezing and thawing using a cryogenic light microscope. There was no obvious link between taxonomic position and their morphological response to freezing and thawing. The viability of seven of these strains was examined following freezing and thawing in the presence or absence of the cryoprotectants glycerol and dimethyl sulphoxide. Intracellular ice and hyphal shrinkage were not necessarily lethal events, but in many cases they affected the rate and quality of growth. Both cryoprotectants reduced shrinkage, shifted the cooling rate where intracellular ice formed in many cases, and improved the recovery of strains. The results presented aid the development of successful cryopreservation protocols.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1023/A:1008820432471
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  • 7
    Electronic Resource
    Electronic Resource
    The use of analytic surfaces for the design of centrifugal impellers by computer graphicsThis paper is Crown copyright and is reproduced with the permission of the Controller, Her Majesty's Stationery Office. (1973)
    Chichester [u.a.] : Wiley-Blackwell
    International Journal for Numerical Methods in Engineering 7 (1973), S. 137-154 
    Details
    ISSN: 0029-5981
    Keywords: Engineering ; Engineering General
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Mathematics , Technology
    Notes: This paper shows how analytic surfaces can be used to define the vanes of an impeller of a centrifugal compressor. The analysis has been given of a cubic-linear patch for representing three-dimensional geometries. An example has shown how this type of patch can be successfully used to model the geometry of centrifugal or mixed flow impellers having 'swept back vanes at the outlet.The analysis has been programmed for the Atlas computer at the Computer Aided Design Centre (CADC), Cambridge and set up in such a way that the designer can examine systematically a range of impeller shapes.
    Additional Material: 11 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/nme.1620070205
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  • 8
    Electronic Resource
    Electronic Resource
    BOUNDARY ELEMENT ANALYSIS OF LINEAR THERMOELASTIC CONSOLIDATION (1996)
    New York, NY [u.a.] : Wiley-Blackwell
    International Journal for Numerical and Analytical Methods in Geomechanics 20 (1996), S. 457-488 
    Details
    ISSN: 0363-9061
    Keywords: Linear thermo-poro-elastic materials ; boundary element method ; Green's functions ; Dirac delta functions ; Engineering ; Engineering General
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Architecture, Civil Engineering, Surveying , Geosciences
    Notes: This paper presents a direct boundary element method of numerical analysis, formulated in the Laplace transform domain, for a plane strain analysis of a linear thermo-poro-elastic material consolidating in the presence of a heat source. The equations governing the behaviour of the material are assumed to be a set of self-adjoint and fully coupled linear equations. A physical intepretation of the constants appearing in the linear theory relevant to engineering applications is presented. A boundary integral equation is developed from the governing equations in a straightforward way using the properties of Dirac delta functions, and an approximate boundary element method of numerical analysis is implemented using the Green's functions derived previously by the authors. The numerical analysis presented is motivated by the engineering design of a heat generating radioactive waste repository located deep underground. For this reason, there is a description of the application of the boundary integral equation method presented to the numerical solution of several problems of theoretical and practical interest in the area of radioactive waste disposal in clay-like soils.
    Additional Material: 20 Ill.
    Type of Medium: Electronic Resource
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    Paper (German National Licenses)
  • 9
    Electronic Resource
    Electronic Resource
    Boundary integral analysis of transient thermoelasticity (1989)
    New York, NY [u.a.] : Wiley-Blackwell
    International Journal for Numerical and Analytical Methods in Geomechanics 13 (1989), S. 283-302 
    Details
    ISSN: 0363-9061
    Keywords: Engineering ; Engineering General
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Architecture, Civil Engineering, Surveying , Geosciences
    Notes: A simple and efficient method for the solution of uncoupled transient thermoelastic problems using boundary integral techniques is presented. The method employs a Laplace transformation to remove temporarily the time dependence of the governing equations. Numerical analysis is then carried out in the transform space, and results in the time-position space are found by numerical inversion of the Laplace transform. The method has the advantage that it avoids time-stepping and the costly evaluation of domain integrals. Boundary element and analytic solutions are compared, and the effect of cooling on the stresses around a deep underground opening is examined.
    Additional Material: 8 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/nag.1610130305
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