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  • 1
    Electronic Resource
    Electronic Resource
    Rapid detection of hemoglobin variants by mutagenically separate polymerase chain reaction (MS-PCR) (1995)
    Springer
    Annals of hematology 71 (1995), S. 97-100 
    Details
    ISSN: 1432-0584
    Keywords: Hemoglobin variant ; MS-PCR ; Allele-specific primer
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract The detection of molecular defects of hemoglobin variants using mutagenically separated polymerase chain reaction (MS-PCR) was applied in this study. Using different lengths of allele-specific mutagenic primers, normal and mutant alleles of hemoglobin genes were amplified in the same reaction tube. Subsequent gel electrophoresis showed at least one of the two allelic products at the same loci or at least two of the several allelic products at different loci. We employed MS-PCR to test the following hemoglobin variants: Hb Constant Spring (Hb CS), Hb E, Hb G-Taichung, Hb J-Meinung, and Hb Kaohsiung. The results were the same as those obtained by amplified created reaction sites (ACRS) or direct sequencing. We conclude that the MS-PCR provides a rapid and simple alternative to other techniques for mutation detection in hemoglobin variants. Moreover, the principle can be extended to other genetic diseases.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01699253
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    Paper (German National Licenses)
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  • 2
    Electronic Resource
    Electronic Resource
    High-performance liquid chromatography of carbohydrates in enzymic hydrolysate of waste paper (1992)
    Springer
    Chromatographia 34 (1992), S. 534-536 
    Details
    ISSN: 1612-1112
    Keywords: Column liquid chromatography ; Carbohydrates ; Enzymic hydrolysate ; Waste paper
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology
    Notes: Summary Waste paper was pretreated with 5M sulfuric acid at 28°C to give a gel-like solution which was then hydrolyzed with cellulase, purified fromTrichoderma viride, to form carbohydrates. The determination of the carbohydrates was accomplished using an aminopropylbonded silica HPLC column and an RI detector. The detection limits were 20 ppm; xylose, glucose, and cellobiose were the major components. Salts from the buffer solution may affect the activity of cellulase. The analysis of carbohydrates can be performed under acid conditions. HPLC analysis of carbohydrates from enzymatic hydrolysis is reliable and successful.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02290248
    Library Location Call Number Volume/Issue/Year Availability
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    Paper (German National Licenses)
    Fulltext
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