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  • 1
    Electronic Resource
    Electronic Resource
    Analysis of the STR myelin basic protein locus in Koreans (1997)
    Springer
    International journal of legal medicine 110 (1997), S. 173-174 
    Details
    ISSN: 1437-1596
    Keywords: Key words STR ; Myelin basic protein ; Koreans
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine , Law
    Notes: Abstract Allele und genotype frequencies and the mutation rate of a short tandem repeat locus, the myelin basic protein (MBP) gene, were studied in 973 unrelated Koreans. The alleles were distributed in two discrete regions, one in a high molecular weight region (A) above 190 bp and the other in a low molecular weight region (B) below 150 bp. In a heterozygote, two alleles were found in each region. In region A 13 alleles were found and in region B 7 alleles. Most alleles showed a difference of 4 bp, but three interalleles were found in region A. Allele frequencies in Koreans differed from those reported for Germans and Portuguese. Sets of alleles, one from each region, were linked and transmitted to the offspring. A total of 36 haplotypes and 148 genotypes was identified. In 763 gametes of 550 families, whose parent-child relationship was confirmed using other serological and DNA systems, all alleles were transmitted in a Mendelian fashion, and no mutations were observed. The polymorphism information content (PIC) in Koreans was calculated as 0.833 for region A and 0.718 for region B. The power of discrimination (PD) was 0.959 for region A and 0.901 for region B. No significant deviation from Hardy-Weinberg equilibrium could be observed for this system.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s004140050060
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    Paper (German National Licenses)
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  • 2
    Electronic Resource
    Electronic Resource
    PhoB-dependent transcriptional activation of the iciA gene during starvation for phosphate in Escherichia coli (1999)
    Springer
    Molecular genetics and genomics 262 (1999), S. 448-452 
    Details
    ISSN: 1617-4623
    Keywords: Key words Replication initiation ; iciA ; Phosphate regulon ; PhoB ; Escherichia coli
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The IciA protein from Escherichia coli has been shown specifically to inhibit the in vitro initiation of chromosomal DNA replication. However, the in vivo role of IciA has not yet been established. In order to investigate the in vivo function of this protein, expression of the iciA gene was studied by monitoring the β-galactosidase activity specified by an iciA promoter-lacZ fusion inserted into the chromosome. Among the conditions tested (carbon starvation, the stringent response, phosphate starvation, and the SOS response), only phosphate depletion increased iciA expression. Supplementation of phosphate-depleted cultures with inorganic phosphate reduced the β-galactosidase activity to basal levels. Enhanced expression of iciA-lacZ was dependent upon the PhoB protein. PhoB is known to be a transcriptional activator of the Pho regulon, expression of which is activated during phosphate starvation. It was also found that the iciA promoter contains a PhoB protein-binding sequence, termed the Pho box, which is necessary for the activation of genes of the Pho regulon. These results suggest that the iciA gene is a member of the Pho regulon.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s004380051104
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    Paper (German National Licenses)
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  • 3
    Electronic Resource
    Electronic Resource
    Comparative behaviour of L-929 fibroblastic and human endothelial cells onto crosslinked protein substrates (1990)
    Springer
    Cytotechnology 3 (1990), S. 259-269 
    Details
    ISSN: 1573-0778
    Keywords: crosslinked albumin gelatin ; cytocompatibility ; endothelial cells ; fibroblastic cell
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Abstract Studies were carried out to compare the behaviour of human umbilical vein endothelial cells (HUVEC) and L-929 fibroblastic cells towards proteins crosslinked by glutaraldehyde (GTA) or carbodiimide (CDI) proposed for coating of vascular prostheses. CDI crosslinking of bovine serum albumin used alone, or mixed with gelatin, allowed higher rates of cell growth and DNA synthesis than GTA crosslinking independent of cells. Assessment of the plating efficiency revealed a similar behaviour of both cells towards membranes and reference plastic surface in terms of percentages of bound cells. HUVEC proliferation onto CDI crosslinked gelatin and/or albumin membranes did not differ significantly whereas the growth of L-929 was enhanced onto gelatin albumin membranes in comparison with both gelatin membranes and the reference surface. The analysis of DNA synthesis corroborated the results of the growth curves and elicited a delay of the growth phases in HUVEC cultured onto CDI crosslinked membranes, unlike the L-929 fibroblast.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00365490
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    Paper (German National Licenses)
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