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  • Extracellular matrix vesicles  (1)
  • Inorganic Chemistry  (1)
  • 1
    Electronic Resource
    Electronic Resource
    Correlative morphometric and biochemical analysis of purified extracellular matrix vesicles from rat alveolar bone (1983)
    Springer
    Calcified tissue international 35 (1983), S. 320-326 
    Details
    ISSN: 1432-0827
    Keywords: Extracellular matrix vesicles ; Vesicular fractional area ; Morphometric ; Purification
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine , Physics
    Notes: Summary Extracellular matrix vesicles were isolated from rat alveolar bone and purified by either gel filtration or discontinuous sucrose density gradient. Morphometric evaluation of electronmicrographs of pellets of purified and nonpurified vesicle fractions was correlated with the acitvity of vesicular enzymes. A high correlation was found between the percentage of area occupied by vesicles with electron-dense content (electron-dense vesicle fractional area) and the enzymatic activity. Highest enzymatic specific activities and electrondense vesicle fractional area were recorded in the “light” vesicle-enriched fraction obtained after equilibrium density centrifugation. These parameters revealed lowest values in the “heavy” vesicle-enriched fraction resolved by the same methods. The combined electron-dense and electron-lucent fractional area (vesicular fractional area) was similar in the different purified fractions. It is therefore suggested that the fraction obtained by gel filtration contains both “light” and “heavy” vesicles. Morphometric study is proposed as an additional criterion for the degree of purification of matrix vesicle preparations.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02405053
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    Paper (German National Licenses)
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  • 2
    Electronic Resource
    Electronic Resource
    Über die Verschiebung des chemischen Gleichgewichtes an Grenzflächen (1927)
    Weinheim : Wiley-Blackwell
    Berichte der deutschen chemischen Gesellschaft 60 (1927), S. 1036-1039 
    Details
    ISSN: 0365-9631
    Keywords: Chemistry ; Inorganic Chemistry
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/cber.19270600505
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    Paper (German National Licenses)
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