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  • 1
    Electronic Resource
    Electronic Resource
    Correlative morphometric and biochemical analysis of purified extracellular matrix vesicles from rat alveolar bone (1983)
    Springer
    Calcified tissue international 35 (1983), S. 320-326 
    Details
    ISSN: 1432-0827
    Keywords: Extracellular matrix vesicles ; Vesicular fractional area ; Morphometric ; Purification
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine , Physics
    Notes: Summary Extracellular matrix vesicles were isolated from rat alveolar bone and purified by either gel filtration or discontinuous sucrose density gradient. Morphometric evaluation of electronmicrographs of pellets of purified and nonpurified vesicle fractions was correlated with the acitvity of vesicular enzymes. A high correlation was found between the percentage of area occupied by vesicles with electron-dense content (electron-dense vesicle fractional area) and the enzymatic activity. Highest enzymatic specific activities and electrondense vesicle fractional area were recorded in the “light” vesicle-enriched fraction obtained after equilibrium density centrifugation. These parameters revealed lowest values in the “heavy” vesicle-enriched fraction resolved by the same methods. The combined electron-dense and electron-lucent fractional area (vesicular fractional area) was similar in the different purified fractions. It is therefore suggested that the fraction obtained by gel filtration contains both “light” and “heavy” vesicles. Morphometric study is proposed as an additional criterion for the degree of purification of matrix vesicle preparations.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02405053
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  • 2
    Electronic Resource
    Electronic Resource
    Modulation of matrix vesicle enzyme activity and phosphatidylserine content by ceramic implant materials during endosteal bone healing (1992)
    Springer
    Calcified tissue international 51 (1992), S. 429-437 
    Details
    ISSN: 1432-0827
    Keywords: Ceramic implants ; Matrix vesicles ; Bone healing ; Alkaline phosphatase ; Phospholipase A2
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine , Physics
    Notes: Summary This study examined effects of bone bonding and nonbonding implants on parameters associated with matrix vesicle-mediated primary bone formation, matrix vesicle alkaline phosphatase and phospholipase A2 specific activities, and phosphatidylserine content. Tibia marrow ablation followed by implantation of KG-Cera, Mina 13 (bonding), KGy-213, or M 8/1 (nonbonding) was used as the experimental model. Postsurgery, matrix vesicle-enriched microsomes (MVEM) were isolated from implanted and contralateral limbs. MVEM alkaline phosphatase and phospholipase A2 were stimulated adjacent to bonding implants with similar, though reduced, effects contralaterally. Alkaline phosphatase exhibited slight stimulation in nonbonding tissue; phospholipase A2 was inhibited or unchanged in treated and contralateral limbs. Phosphatidylserine content of MVEM was differentially affected by the implant materials. Thus, MVEM are modulated by implant materials locally and systemically. The data demonstrate that the model is a biologically relevant diagnostic for assessing the tissue/implant interface, primary calcification is affected by implant materials, and implant-specific effects are detected in the contralateral unimplanted limb.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00296676
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  • 3
    Electronic Resource
    Electronic Resource
    Regulation of arachidonic acid turnover by 1,25-(OH)"2D"3 and 24,25-(OH)"2D"3 in growth zone and resting zone chondrocyte cultures
    Amsterdam : Elsevier
    Biochimica et Biophysica Acta (BBA)/Biomembranes 1027 (1990), S. 278-286 
    Details
    ISSN: 0005-2736
    Keywords: 1,25-Dihydroxyvitamin D-3 ; 24,25-Dihydroxyvitamin D-3 ; Arachidonic acid turnover ; Chondrocyte ; Matrix vesicle ; Phospholipid ; Vitamin D-3
    Source: Elsevier Journal Backfiles on ScienceDirect 1907 - 2002
    Topics: Biology , Chemistry and Pharmacology , Medicine , Physics
    Type of Medium: Electronic Resource
    URL: http://linkinghub.elsevier.com/retrieve/pii/0005-2736(90)90319-J
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  • 4
    Electronic Resource
    Electronic Resource
    Soluble antagonists to interleukin-1 (IL-1) and tumor necrosis factor (TNF) inhibits loss of tissue attachment in experimental periodontitis (2001)
    Copenhagen : Munksgaard International Publishers
    Journal of clinical periodontology 28 (2001), S. 0 
    Details
    ISSN: 1600-051X
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Medicine
    Notes: Background, aims: Periodontal disease is a significant cause of tooth loss among adults and is characterized by the alteration and permanent destruction of the deeper periodontal tissues. Although the presence of pathologic microbes is required to trigger this process, the amplification and progression of the diseased state is believed to rely heavily on the production of host mediators in response to bacteria or their metabolic products. The inflammatory response is effective in preventing large-scale colonization of the gingival tissues by bacteria that lie in close proximity to the tooth surface or within the gingival sulcus. It has been postulated that the host-response in some individuals may lead to an over-reaction to invading oral pathogens resulting in the destruction of periodontal tissues.Methods: Several host-derived mediators are believed to contribute to this response. Two agents considered to be essential in periodontal destruction are interleukin-1 (IL-1) and tumor necrosis factor (TNF). We investigated the role of IL-1 and TNF in the loss of connective tissue attachment in a Macaca fascicularis primate model of experimental periodontitis. Silk ligatures impregnated with the periodontal pathogen, Porphyromonas gingivalis were wrapped around the posterior teeth and the activity of IL-1 and TNF were inhibited by soluble receptors to these proinflammatory cytokines via local injection into interdental papillae.Results: Histomorphometric analysis indicates that IL-1 and TNF antagonists significantly reduced the loss of connective tissue attachment by approximately 51% and the loss of alveolar bone height by almost 91%, both of which were statistically significant.Conclusion: This investigation demonstrates that the loss of connective tissue attachment and progression of periodontal disease can be retarded by antagonists to specific host mediators such as IL-1 and TNF and may provide a potential treatment modality to combat the disease process.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1034/j.1600-051x.2001.028003233.x
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  • 5
    Electronic Resource
    Electronic Resource
    Human histology of new attachment after root coverage using subepithelial connective tissue graft (2001)
    Copenhagen : Munksgaard International Publishers
    Journal of clinical periodontology 28 (2001), S. 0 
    Details
    ISSN: 1600-051X
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Medicine
    Notes: Background, aims: Connective tissue grafts are used successfully in periodontal therapy for root coverage. However, reports on the histologic interface between the root surface and the grafted tissue have been few in number. This report describes a case study in which a subepithelial connective tissue graft was placed in a 27-year-old female on the maxillary left side.Methods: The graft (15 mm long, 10 mm wide, 1.5 mm thick) included palatal periosteum and was placed with the periosteal side facing the exposed bone and root surfaces.Results: 15 weeks after grafting, the teeth presented with residual recessions of 1 mm, and buccal probing depths were approximately 1 mm. 14 months post-surgery, the 1st maxillary premolars on both sides were extracted for orthodontic therapy. Clinical parameters at the graft site remained as at 15 weeks. Histologic analysis of tooth #24 showed that the sulcular epithelium was keratinized; epithelium lining the dentin exhibited rete ridges projecting into the gingival connective tissue; and junctional epithelium extended over new cementum. New connective tissue attachment was also observed, including periodontal ligament.Conclusion: Biological width was comparable pre- and post-surgery, indicating a real gain in attachment of 3.9 mm.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1034/j.1600-051x.2001.028007657.x
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  • 6
    Electronic Resource
    Electronic Resource
    Distribution of extracellular matrix vesicles in healing rat tibial bone
    Amsterdam : Elsevier
    Ultramicroscopy 19 (1986), S. 387 
    Details
    ISSN: 0304-3991
    Source: Elsevier Journal Backfiles on ScienceDirect 1907 - 2002
    Topics: Electrical Engineering, Measurement and Control Technology , Natural Sciences in General , Physics
    Type of Medium: Electronic Resource
    URL: http://linkinghub.elsevier.com/retrieve/pii/0304-3991(86)90099-9
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  • 7
    Electronic Resource
    Electronic Resource
    1,25-(OH)"2D"3 and 24,25-(OH)"2D"3 regulation of arachidonic acid turnover in chondrocyte cultures is cell maturation-specific and may involve direct effects on phospholipase A"2
    Amsterdam : Elsevier
    Biochimica et Biophysica Acta (BBA)/Molecular Cell Research 1136 (1992), S. 45-51 
    Details
    ISSN: 0167-4889
    Keywords: 1,25-(OH)"2D"3 ; 24,25-(OH)"2D"3 ; Arachidonic acid turnover ; Cell differentiation ; Chondrocyte ; Phospholipase A"2
    Source: Elsevier Journal Backfiles on ScienceDirect 1907 - 2002
    Topics: Biology , Chemistry and Pharmacology , Medicine , Physics
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1016/0167-4889(92)90083-N
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  • 8
    Electronic Resource
    Electronic Resource
    Enzyme activities in the androgenized rat uterus refractory to oestrogenic stimulation
    Amsterdam : Elsevier
    Journal of Steroid Biochemistry 25 (1986), S. 491-496 
    Details
    ISSN: 0022-4731
    Source: Elsevier Journal Backfiles on ScienceDirect 1907 - 2002
    Topics: Biology , Chemistry and Pharmacology
    Type of Medium: Electronic Resource
    URL: http://linkinghub.elsevier.com/retrieve/pii/0022-4731(86)90393-6
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  • 9
    Electronic Resource
    Electronic Resource
    Proliferation, differentiation, and protein synthesis of human osteoblast-like cells (MG63) cultured on previously used titanium surfaces (1996)
    Copenhagen : Munksgaard International Publishers
    Clinical oral implants research 7 (1996), S. 0 
    Details
    ISSN: 1600-0501
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Medicine
    Notes: This study compared osteoblast proliferation, differentiation, and protein synthesis on new and used titanium (Ti) disks to test the hypothesis that cleaning and resterilization of previously used Ti disks does not alter cell response to a particular surface. Ti disks of varying roughness were prepared by one of five different treatment regimens. Standard tissue culture plastic was used as a control. Human osteoblast-like cells (MG63) were cultured on the Ti disks and cell proliferation, cell differentiation, RNA synthesis and matrix production (collagen and noncollagen protein; proteoglycans) measured. After their first use, the disks were cleaned, re-sterilized by autoclaving. and MG63 cells cultured on them as before. At confluence, the same parameters were measured and cell behaviour on new and used disks compared. When confluent cultures of cells on plastic were compared to those cultured on new Ti surfaces, cell number was reduced on the roughest surfaces and equivalent to plastic on the other surfaces. Cell number was further reduced when disks with the roughest surfaces were re-used; no differences in cell number could be discerned after cleaning and re-sterilization. Cell proliferation was inversely related to surface roughness and was less than seen on tissue culture plastic. Re-use of the Ti disks resulted in no change in cell proliferation rate. Alkaline phosphatase specific activity in isolated cells was lowest on the rougher surfaces; no differences between new and used disks were observed. Similarly, enzyme activity in the cell layer was decreased in cultures grown on rougher surfaces, with no effect of prior disk use being noted. RNA synthesis was decreased with respect to plastic in cultures on smoother surfaces and increased on rougher surfaces; prior disk use did not alter RNA synthesis. Collagen production by the cells was decreased on smoother surfaces, but was comparable to tissue culture plastic when grown on rougher surfaces. Non-collagen protein production was unaffected by culture surface and whether or not the disk had been previously used. Proteoglycan synthesis by cells was decreased on all surfaces studied and comparable on both new and used disks. The results of this study indicate that Ti implant surfaces are unaffected by cleaning and resterilization, although rougher surfaces may require more extensive cleaning than smoother ones. This suggests the possibility that implants, in the same patient, could be safely reused. In vivo studies in animals, however, need to be performed before clinical application can be considered.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1034/j.1600-0501.1996.070104.x
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  • 10
    Electronic Resource
    Electronic Resource
    Markers of primary mineralization are correlated with bone-bonding ability of titanium or stainless steel in vivo (1995)
    Copenhagen : Munksgaard International Publishers
    Clinical oral implants research 6 (1995), S. 0 
    Details
    ISSN: 1600-0501
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Medicine
    Notes: Critical events in the adaptation of osseous tissues to implant materials involve initial calcification of the newly synthesized bone. Previous studies indicated that bone-bonding but not nonbonding glass ceramics increase the matrix vesicle number, thereby compensating for delayed maturation of the extracellular organelles. The present study assessed whether this was also true for metal implants commonly used in orthopaedics and oral medicine. Bone-bonding titanium (Ti) or nonbonding stainless steel (SS) implants were placed in the right tibias of Sabra rats following ablation of the marrow. At 3, 6, 14, and 21 days postinjury, newly formed endosteal bone in the treated and contralateral limbs was removed and matrix vesicle-enriched membranes isolated. Alkaline phosphatase and phospholipase A2 specific activities and phosphatidylserine (PS) content were determined and compared with those of a nonsurgical control group. Results show that matrix vesicle alkaline phosphatase and phospholipase A2 activity and PS content was increased in the Ti-implanted limbs at 6 (peak). 14, and 21 days, although at levels less than observed in normal ealing. Alkaline phosphatase activity remained elevated throughout the healing period. In contrast, these parameters were markedly inhibited in the SS-implanted limbs with respect to Ti or to normal healing. Both implants altered the systemic response associated with marrow ablation. but in an implant-specific manner. The results support the hypothesis that cells adjacent to bone-bonding materials can compensate for negative effects on primary mineralization during osteogenesis, whereas cells adjacent to nonbonding materials either do not compensate or are further depressed. The data support the use of the rat marrow ablation model as a tool for rapid, initial assessment of biomaterials in bone.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1034/j.1600-0501.1995.060101.x
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