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  • 1
    Electronic Resource
    Electronic Resource
    Springer
    Journal of bioenergetics and biomembranes 14 (1982), S. 479-498 
    ISSN: 1573-6881
    Keywords: Oxidative phosphorylation ; F1-ATPase ; nucleotide binding sites ; cooperativity ; nucleotide analogs ; fluorescence ; mechanism
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology , Physics
    Notes: Abstract The present study contributes to the problem of the dynamic structure of mitochondrial F1-ATPase and the functional interrelation of so-called tight nucleotide binding sites. Nucleotide analogs are used as a tool to differentiate two distinct functional states of the membrane-bound enzyme, proposed to reflect corresponding conformational states; they reveal F1-ATPase as a “dual-state” enzyme: ATP-synthetase, and ATP-hydrolase. The analogs used are 3′-naphthoyl esters of AD(T)P, and 2′(3′)-O-trinitrophenyl ethers of AD(T)P. Both types of analogs act inversely to each other with respect to their relative effects on oxidative phosphorylation and on ATPase in submitochondrial vesicles. The respective ratios ofK i versus both processes are 250/1 compared to 1/170. It is also shown that in the presence of the inhibitory 3′-esters oxidative phosphorylation deviates from linear kinetics and that these inhibitors induce a lag time of oxidative phosphorylation depending on the initial pattern of nucleotides available to energized submitochondrial vesicles. The duration of the lag time coincides with the time course of displacement of the analog from a tight binding site. The conclusions of the study are: (a) the catalytic sites of F1-ATP-synthetase are not operating independently from each other; they rather interact in a cooperative manner; (b) F1-ATPase as a “dual-state” enzyme exhibits highly selective responses to tight binding of nucleotides or analogs in its “energized” (membrane-bound) state versus its “nonenergized” state, respectively.
    Type of Medium: Electronic Resource
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  • 2
    ISSN: 1435-2451
    Keywords: Burn injuries ; Mesh graft ; Fibrin glue ; Verbrennung ; Mesh-Graft ; Fibrinkleber
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Description / Table of Contents: Zusammenfassung Experimentelle Verbrennungswunden an der Rückenhaut von Ratten (n = 20) im Bereich des thoracolumbalen Übergangs wurden mit Haut-Mesh-graft gedeckt, wobei in der Versuchsgruppe zusätzlich Fibrinkleber (Tissuecol) verwendet wurde. Planimetrische Auswertungen der nach 14 Tagen verbleibenden Wundflächen sowie mikroangiographische, histologische und thermographische Untersuchungen zeigen ein verbessertes Einheilen des Transplantates bei gleichzeitiger Fibrinkleberapplikation, was durch den festen initialen Kontakt zum Wundgrund und die verbesserte Blutstillung erklärt werden kann.
    Notes: Summary Experimental bum injuries in the dorsal thoracolumbar region of rats were covered by skin mesh grafts. In the treated group, fibrin adhesive (Tissucol) was also applied. Fourteen days after transplantation planimetric localization of the remaining wound area, microangiographic, histologic and thermographic studies demonstrated a better healing of the transplant additionally treated with fibrin adhesive. This can be explained by the fibrin-initiated contact with the wound and the hemostyptic effect of the fibrin adhesive.
    Type of Medium: Electronic Resource
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