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  • 1
    Electronic Resource
    Electronic Resource
    Springer
    Surgical and radiologic anatomy 10 (1988), S. 101-106 
    ISSN: 1279-8517
    Keywords: Sonography ; Mimetic musculature ; Anatomic clinical study ; Facial palsy
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Description / Table of Contents: Résumé Des études anatomiques antérieures nous ont permis d'identifier et de différencier chacun des muscles de la mimique. Secondairement, nous avons analysé les possibilités offertes par les ultrasons pour visualiser la musculature faciale chez 15 volontaires. Cette méthode a également été utilisée dans le bilan diagnostic de certains états pathologiques, notamment les paralysies faciales. Les excellents renseignements obtenus sur la musculature faciale montrent que cette technique peut être utile à la fois au diagnostic précis de la paralysie faciale et à la décision thérapeutique plastique ou reconstructive ainsi qu'à la surveillance à distance.
    Notes: Summary Primary anatomic studies served for identification and differentiation of the individual mimetic muscles. As a second step, we investigated the clinical potential of ultrasound imaging to visualize the mimetic musculature in 15 volunteers. This examination technique was used to diagnose pathological alterations, especially associated with facial palsy. The excellent sonographic visualization of the mimetic musculature indicates that this technique may be a valuable adjunct in the diagnosis and differentiation of facial palsy, as well as in planning plastic surgery and reconstructive procedures, and in follow-up care.
    Type of Medium: Electronic Resource
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  • 2
    ISSN: 1434-4726
    Keywords: Key words Oral mucosa ; Keratinocyte isolation ; Immunomagnetic beads ; Cytokine assay
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract In studying human oral keratinocytes, it would be very helpful to obtain a pure population of cells without prior in vitro expansion. An immunomagnetic separation technique, or magnetic cell separation (MACS), was modified for efficient purification of human oral keratinocytes. Subsequent to two-step enzymatic digestion, the cell suspension was labelled with a mouse anti-CD45 (pan-leukocyte) monoclonal antibody (MoAb) to stain mononuclear cells. In a second step a rat anti-mouse antibody conjugated with colloidal superparamagnetic particles was used. Labelled cells were retained in the magnetic field of a permanent magnet on columns containing a ferromagnetic matrix. The unlabelled, unretained cells were further examined by flow cytometry analysis, enzyme-linked immunosorbent assay and polymerase chain reaction. After the MACS procedure, unretained cells showed a strong positivity for the lu-5 MoAb (as a marker for pan-cytokeratin) and were negative for anti-vimentin (to mark mesenchymal cells), for anti-CD45 MoAb and for melanocyte-detecting antibodies, thus representing pure keratinocytes (〉 98%). Purified keratinocytes maintained full viability (〉 91%) and functional capacities. [3H]thymidine uptake and epidermal growth factor (EGF) receptor expression were unaltered when compared with the non-separated cell population. Furthermore, interleukin-1α was detected at the protein and RNA levels in keratinocytes immediately after MACS enrichment. Our findings show that MACS appears to be a useful tool for purification of oral keratinocytes and allows for further functional studies without prior subcultivation of cells.
    Type of Medium: Electronic Resource
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