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  • 1
    Electronic Resource
    Electronic Resource
    Springer
    Annals of hematology 39 (1979), S. 333-344 
    ISSN: 1432-0584
    Keywords: Erythrozytenfiltrierbarkeit ; Papierfilter ; Filterpapier-Kalibrierung ; Filterability of red cells ; Paper filters ; Calibration of filter papers
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Description / Table of Contents: Summary A method for the assessment of red cell filterability with paper filters is described. Two milliliters of a washed red cell suspension with a hematocrit of 50 % was filtered through a filter paper cone in a glass funnel and the filtration half-time (FT 1/2) was measured. The filter papers were calibrated by previous measurement of the filtration time for the suspending solution, the calibration time (CT). A linear correlation between CT and FT 1/2 was found for individual red cell suspensions (p 〈 0.001) as well as for a normal population (p 〈 0.001). Consequently, the index FT1/2/CT should represent a more reproducible parameter than FT1/2 and experimentally this appears to be the case. In fact, elimination of erratic values in a normal population was realized. Furthermore, discrimination between normal and abnormal values was improved and the coefficient of variation of single measurements passed from 28.4 to 9.4%. The reproducibility and reliability of this extremely simple method are therefore decisively enhanced without complication of the procedure.
    Notes: Zusammenfassung Es wird eine Methode zur Untersuchung der Erythrozytenfiltrierbarkeit mit Papierfiltern beschrieben. Zwei ml einer gewaschenen Erythrozytensuspension mit HÄmatokrit 50% wurden durch einen Papierfilter in einem Glastrichter filtriert und die Filtrationshalbwertzeit (FT 1/2) gemessen. Zuvor erfolgte die Kalibrierung der Filterpapiere durch Messung der Filtrationszeit für die Suspensionslösung (Kalibrierungszeit, CT). Zwischen FT/2 und CT zeigte sich eine positive lineare Korrelation sowohl für die einzelne Erythrozytensuspension (p 〈 0,001) als auch für ein gesundes Kollektiv (p 〈 0,001). Deshalb sollte der Index FT1/2/CT einen besser reproduzierbaren Parameter darstellen als FT1/2. TatsÄchlich wurden damit erratische Werte in einem gesunden Kollektiv eliminiert, die Unterscheidung von normalen und pathologischen Werten wurde sicherer und der Variationskoeffizient der Einzelmessung sank von 28,4 auf 9,4 %. Reproduzierbarkeit und VerlÄ\lichkeit dieser extrem einfachen Methode wurden somit ohne Komplizierung des technischen Vorgehens entscheidend verbessert.
    Type of Medium: Electronic Resource
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  • 2
    ISSN: 1439-6327
    Keywords: Creatine kinase ; Kinetics ; Myocardial infarction ; Running ; Age
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary Following a 100 km race creatine kinase (CK) creatine kinase MB (CKMB) activities were serially measured in well trained athletes and compared with enzyme activities in patients with acute myocardial infarction (AMI). The half-time of disappearance of CK (CKt 1/2) was 1.75±0.70 days in runners who trained within the 1st week after the race, and was 0.81±0.18 days in patients with AMI, P〈0.005. CKt 1/2 in runners was shorter (1.17±0.28 days) when no training was performed in the first postrace week. CKt 1/2 was linearly correlated with age (P〈0.01) in the runners but not in the patients. CKMBt 1/2 was 1.30 and 1.11 days in two runners and 0.56±0.10 days in patients with AMI (P〈0.05). In line with histologic and enzymatic findings in skeletal muscle of long distance runners as reported by other workers, our kinetic data provide further evidence that CK and CKMB are released from muscular compartments in runners other than those in patients with AMI.
    Type of Medium: Electronic Resource
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