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  • Firmness  (1)
  • Genes GOT-1  (1)
  • Genes PRX-1, PRX-2, PRX-3, PRX-4, PRX-5, PRX-6, PRX-7, PRX-8  (1)
  • Genes Sod-1  (1)
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Keywords
  • 1
    ISSN: 1432-2242
    Keywords: Key words Apple ; Fruit ; Firmness ; Texture ; QTL analysis ; Genes
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract  Texture is a major component of consumer preference for eating-quality in apple. A quantitative genetic analysis of traits associated with fruit-flesh firmness was carried out. This was based on segregation in an unselected mapping population replicated at six sites and harvested over 2 years. Different methods of assessment were compared, and a principal components analysis carried out. Instrumental measures used were Magness-Taylor penetrometer readings, stiffness by acoustic resonance, and a range of sensory descriptors assessed by a trained panel. There were good correlations between some measures, although stiffness was poorly correlated. Whilst genotype by environment effects were large, significant effects were attributable to the genotype, and these were used to detect QTLs. Significant QTLs were detected on seven linkage groups, with large effects on linkage groups L01, L10 and L16. Whilst there was a poor correlation between acoustic stiffness and other measures, the significant and suggestive QTL detected for stiffness on linkage group L10 did represent a subset of significant QTLs detected for the penetrometer measure. The use of sensory assessment proved valuable in detecting QTLs representing different attributes of fruit texture. The possibility of interaction between significant QTLs for fruit texture and other strongly selected traits such as scab resistance and fruit acidity is addressed.
    Type of Medium: Electronic Resource
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  • 2
    Electronic Resource
    Electronic Resource
    Springer
    Theoretical and applied genetics 74 (1987), S. 154-161 
    ISSN: 1432-2242
    Keywords: Malus pumila Mill ; Glutamate oxalo-acetate transaminase ; Incompatibility ; Genes GOT-1 ; IDH-1 ; S ; Linkage
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Four zones of enzymatic activity for glutamate oxaloacetate transaminase (GOT) were found in apple tissue. A dimeric gene, GOT-1, determining the fastest migrating zone, was identified. Six alleles were found, including a near null allelle which produced detectable heterodimeric bands but not homodimeric bands. A marked deficit or absence of certain geno-types in all backcrosses and in some crosses between unrelated varieties was attributed to the close linkage (r=0.02±0.005) of GOT-1 with the incompatibility S locus. GOT-1 was also closely linked with the isocitrate dehydrogenase locus IDH-1 (0.03±0.01). Proposed incompatibility genotypes for four cultivars, and the linked GOT-1 alleles are ‘Cox’: S 1 b/S 2 d, ‘Idared’: S 3 a/S 4 c, ‘Fiesta’: S 3 a/S 2 d and ‘Kent’: S 3 a/S 1 b.
    Type of Medium: Electronic Resource
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  • 3
    Electronic Resource
    Electronic Resource
    Springer
    Theoretical and applied genetics 95 (1997), S. 484-489 
    ISSN: 1432-2242
    Keywords: Key words Malus pumila Mill ; Superoxide dismutase ; Genes Sod-1 ; Sod-3 ; Sod-4 ; Linkage ; Cultivar identification
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract  Four zones of superoxide dismutase (SOD), activity were detected in apple. Genetic studies confirmed the presence of three genes Sod-1, Sod-3 and Sod-4 with three, two and two alleles respectively, including one null allele. One of these genes, Sod-1, was found to be loosely linked to Prx-2 and Prx-3. The Sod-1a allele predominated in all the three groups studied, cultivars, rootstocks and Malus species. The distinct achromatic bands produced by SOD after electrophoresis facilitate its important role with peroxidase (PRX), glutamate oxalacetate transaminase (GOT) and phosphogluconate dehydrogenase (PGD) in the discrimination of apple cultivars.
    Type of Medium: Electronic Resource
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  • 4
    Electronic Resource
    Electronic Resource
    Springer
    Theoretical and applied genetics 83 (1992), S. 392-399 
    ISSN: 1432-2242
    Keywords: Malus pumila Mill ; Peroxidase ; Genes PRX-1, PRX-2, PRX-3, PRX-4, PRX-5, PRX-6, PRX-7, PRX-8 ; Linkage
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Six anodal and two cathodal zones of peroxidase activity were observed in apple and designated PRX-I to PRX-VIII from the most anodal to the most cathodal on the basis of migrational distance. PRX phenotypes were found to be coded by at least eight genes (PRX-1-8); analyses of four (PRX-2, PRX-3, PRX-4 and PRX-7) revealed 15 alleles including three null alleles. PRX-2 and PRX-3 were closely linked, and PRX-1 appeared to be in the same linkage group. In addition, PRX-4 and PRX-5 were linked.
    Type of Medium: Electronic Resource
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