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  • Funkenionen  (2)
  • Inorganic Chemistry  (2)
  • single cell PCR  (2)
  • 1
    Electronic Resource
    Electronic Resource
    Npm/alk fusion mRNA expression in Hodgkin and Reed–Sternberg cells is rare but does occur: Results from single-cell cDNA analysis (1997)
    Springer
    Annals of oncology 8 (1997), S. 83-87 
    Details
    ISSN: 1569-8041
    Keywords: clonal heterogeneity ; Hodgkin's disease ; NPM/ALK ; single cell PCR
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract Background: The translocation t(2;5)(p23;q35) leads to the fusion of the nucleophosmin gene (NPM) on chromosome 5q35 to the recently described receptor kinase ALK 2p23. It is characteristic of a subgroup of CD30+large-cell anaplastic non-Hodgkin's lymphoma (ALCL). Since some cases of Hodgkin's disease (HD) and ALCL share common features, a common pathogenesis has been proposed in a report of the expression of NPM/ALK fusion mRNA in11/13 Hodgkin's lymphomas. Patients and methods: We approached this question by micro manipulatory isolation of single Hodgkin and Reed–Sternberg (H-RS) cells and subsequent RT-PCR amplification of NPM/ALK fusion cDNA from these single cells. Results: Specificity of cell selection was shown by the HD-specific pattern of EBV-gene expression in single H-RS cells. In 4 out of 7 cases, NPM/ALK fusion cDNA was detected in the RNA from whole lymph node tissue. In2 out of 9 cases, NPM/ALK fusion sequences were amplified from single H-RS cells, albeit in a very low frequency (〈5%). Conclusions: These data indicate that NPM/ALK fusion transcripts do not play an early role in the pathogenesis of HD. Whether the rare expression of NPM/ALK is the result of clonal heterogeneity or an indication for clonal evolution and progression toward ALCL can only be answered by the repeated analysis of indicator cases during the course of the disease.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1023/A:1008246719680
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  • 2
    Electronic Resource
    Electronic Resource
    Npm/alk fusion mRNA expression in Hodgkin and Reed–Sternberg cells is rare but does occur: Results from single-cell cDNA analysis (1997)
    Springer
    Annals of oncology 8 (1997), S. 83-87 
    Details
    ISSN: 1569-8041
    Keywords: clonal heterogeneity ; Hodgkin's disease ; NPM/ALK ; single cell PCR
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract Background: The translocation t(2;5)(p23;q35) leads to the fusion of thenucleophosmin gene (NPM) on chromosome 5q35 to the recently describedreceptor kinase ALK 2p23. It is characteristic of a subgroup of CD30+large-cell anaplastic non-Hodgkin's lymphoma (ALCL). Since some cases ofHodgkin's disease (HD) and ALCL share common features, a common pathogenesishas been proposed in a report of the expression of NPM/ALK fusion mRNA in11/13 Hodgkin's lymphomas. Patients and methods: We approached this question by micromanipulatoryisolation of single Hodgkin and Reed–Sternberg (H-RS) cells andsubsequent RT-PCR amplification of NPM/ALK fusion cDNA from these singlecells. Results: Specificity of cell selection was shown by the HD-specificpattern of EBV-gene expression in single H-RS cells. In 4 out of 7 cases,NPM/ALK fusion cDNA was detected in the RNA from whole lymph node tissue. In2 out of 9 cases, NPM/ALK fusion sequences were amplified from single H-RScells, albeit in a very low frequency (〈5%). Conclusions: These data indicate that NPM/ALK fusion transcripts donot play an early role in the pathogenesis of HD. Whether the rare expressionof NPM/ALK is the result of clonal heterogeneity or an indication for clonalevolution and progression toward ALCL can only be answered by the repeatedanalysis of indicator cases during the course of the disease.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1023/A:1008246719680
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  • 3
    Electronic Resource
    Electronic Resource
    Massenspektrometrische Multielementanalyse von Carrara-Marmor (1973)
    Springer
    Fresenius' Zeitschrift für analytische Chemie 267 (1973), S. 186-188 
    Details
    ISSN: 1618-2650
    Keywords: Analyse von Marmor ; Massenspektrometrie ; Funkenionen
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology
    Description / Table of Contents: Zusammenfassung Für Vergleichszwecke wurden Spurenelemente in Carrara-Marmor nach Überführung des Carbonats in ein Oxid bzw. Fluorid bestimmt. Unter Verwendung von experimentell ermittelten relativen Empfindlichkeitsfaktoren für Oxid- bzw. Fluorid-Systeme wurden Konzentrationen für die Elemente Mg, Al, Ti, V, Cr, Mn, Fe, Ni, Cu, As, Rb, Sr, Y, Ba und Ce gefunden, die vom gemeinsamen Mittelwert der Oxid- und Fluorid-Bestimmungen um 〈 20% abweichen.
    Notes: Abstract A comparative study of the oxide and the fluoride system in spark source mass spectrometric analysis was made on a sample of marble from Carrara. Two splits of this carbonate were converted into oxide and fluoride and using experimental relative sensitive factors characteristic for the oxide and fluoride systems, the elements Mg, Al, Ti, V, Cr, Mn, Fe, Ni, Cu, As, Rb, Sr, Y, Ba and Ce in these two splits were determined. The deviation of the average analytical results obtained on these two systems from their mean was 〈 20%.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00428525
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    Paper (German National Licenses)
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  • 4
    Electronic Resource
    Electronic Resource
    Massenspektrometrische Multielement-Analyse von anorganischen Proben in Graphit- und Silber-Pulverelektroden (1974)
    Springer
    Fresenius' Zeitschrift für analytische Chemie 271 (1974), S. 1-6 
    Details
    ISSN: 1618-2650
    Keywords: Analyse von Anorgan. Material ; Massenspektrometrie ; Funkenionen
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology
    Description / Table of Contents: Zusammenfassung Von 32 Elementen wurden im Konzentrationsbereich 1–104ppm (Elementmasse/Elektrodenmasse) relative Empfindlichkeitsfaktoren (REF) für die funkenmassenspektrometrische Multielementanalyse bestimmt. Als leitendes Elektrodenmaterial wurde Graphit- und Silberpulver verwendet. Für zwei Drittel der untersuchten Elemente wurde eine Konzentrationsabhängigkeit der REF gefunden, die durch eine temperaturabhängige Verdampfung und dadurch bedingte Verarmung in der Elektrodenoberfläche erklärt wird. Für die REF in Silber-Pulverelektroden wurden im allgemeinen höhere Werte bei niedrigeren Abfunkzeiten als in Graphitelektroden gefunden. Daraus ergibt sich, daß trotz größerer Inhomogenitäten in der Elektrode im Konzentrationsbereich 〈103 ppm günstiger mit Silberpulver gearbeitet wird.
    Notes: Abstract Relative sensitivity factors (RSF) of 32 elements in the concentration range 1–104 ppm (mass of element/ mass of electrode) have been determined for spark source mass spectrometric analysis. Graphite and silver powder was used as conductive material. The RSF of about two thirds of the elements under investigation were found to be dependent on their concentration in the electrodes. This is thought to be due to volatilization at high temperatures whereby the concentration of these elements at the electrode surfaces is diminished. In general RSF's at silver powder electrodes are higher and necessary sparking times are shorter than those at graphite electrodes. Therefore the use of silver powder for determinations of concentrations 〈103 ppm is advantageous although experimental results show greater scatter due to inhomogeneity of the electrode.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00437481
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  • 5
    Electronic Resource
    Electronic Resource
    Oberflächenzusammensetzung, Löslichkeit und Ionenaktivitätsprodukt von Calcit in fremdionenhaltigen Lösungen (1974)
    Weinheim : Wiley-Blackwell
    Zeitschrift für anorganische Chemie 410 (1974), S. 165-178 
    Details
    ISSN: 0044-2313
    Keywords: Chemistry ; Inorganic Chemistry
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Description / Table of Contents: Surface Composition, Solubility, and the Ion Activity Product of Calcite in Solutions with Auxiliary IonsUsing the method of simultaneous ion and isotope exchange the chemical composition of the surface layer of calcite powder in equilibrium with solutions of varying Me/Ca molar ratios is determined (Me = Mg2+, Co2+, Ni2+, and Fe2+ ions). The ion exchange isotherms show a marked bend in the surface reactivity when a surface molar ration of ∼1 and ∼3 is reached. The solubility of calcite initially decreases on the addition of Co2+ or Ni2+ ions to the solution whereas it increases in the presence of Mg2+ or Fe2+ ions. The activity product of the pseudo compound, CaxMe(1-x)CO3, decreases in the Ni2+, Co2+ or Fe2+ exchange experiments, however, it increases considerably in those with Mg2+ ions.
    Notes: Mit der Methode des gleichzeitigen Ionen- und Isotopenaustausches wird die chemische Zusammensetzung der Oberfläche eines Calcitpulvers im Gleichgewicht mit Lösungen verschiedener Me/Ca- Verhältnisse bestimmt (Me = Mg2+, Co2+, Ni2+, Fe2+). Alle Austauschisothermen zeigen eine deutliche Änderung der Oberflächenreaktivität, wenn eine Zusammensetzung auf der Oberfläche von (Me/Ca)Obfl.∼1 und ∼3 erreicht wird. Die Löslichkeit des Calcits nimmt anfänglich bei Zugabe von Co2+ oder Ni2+ ab, während sie bei Mg2+ oder Fe2+ im ganzen Bereich der Untersuchung zunimmt. Das Ionenaktivitätsprodukt für die „Pseudoverbindung“ in der Oberfläche nimmt in den Ni2+-, Co2+- und Fe2+-Austauschexperimenten ab, in Gegenwart von Mg2+-Ionen jedoch zu.
    Additional Material: 6 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/zaac.19744100210
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  • 6
    Electronic Resource
    Electronic Resource
    Über den roten Phosphor (1908)
    Weinheim : Wiley-Blackwell
    Berichte der deutschen chemischen Gesellschaft 41 (1908), S. 1404-1410 
    Details
    ISSN: 0365-9496
    Keywords: Chemistry ; Inorganic Chemistry
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Additional Material: 6 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/cber.190804101258
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