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  • 1
    Electronic Resource
    Electronic Resource
    Genetic diversity of six isozyme loci in cultivated barley of Tibet (1989)
    Springer
    Theoretical and applied genetics 78 (1989), S. 281-286 
    Details
    ISSN: 1432-2242
    Keywords: Population genetics ; Hordeum vulgare ; Isozyme ; Single-locus genotype ; Multilocus association
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary A random sample of 463 accessions of cultivated barley from the Tibet Hordeum germplasm collection was assayed electorphoretically for genetic diversity at six isozyme loci. Two loci (Acp-1 and Got-1) were found to be monomorphic and extensive variation was detected at the remaining four loci (Est-1, Est-2, Est-3 and Est-4). The allelic composition of Tibetan barley appeared to be distinct as compared to the results of previous studies of barleys from other parts of the world. Partitioning of genetic diversity showed that approximately 96% of the total variation was maintained at the within-subregion level and only about 4% was accounted for by differentiation among the eight subregions. Analysis of multilocus genotypes revealed non-random association of the alleles at the four loci, both in the entire sample and in all the subregions, although the four major multilocus genotypes did not show significant departure from the expectation based on complete random association. The possible causes for the establishment of these multilocus associations were discussed.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00288812
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  • 2
    Electronic Resource
    Electronic Resource
    A method of estimating varietal stability for data of long-term trials (1986)
    Springer
    Theoretical and applied genetics 71 (1986), S. 810-814 
    Details
    ISSN: 1432-2242
    Keywords: Genotype ; environment interaction ; Stability ; Regression ; Reparameterization ; Long-term varietal trials
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary A method is proposed to analyze the stability of cultivars in long-term varietal trials. The method involves the following steps: (i) regress a standard variety on environmental means; (ii) regress varieties under test on the standard variety; (iii) transform, through a procedure of reparameterization, the regression computed for each variety under test on the standard variety into the regression of the variety on environmental means. Although this method is proposed to analyze data sets from complex designs, it may also possess some advantages over conventional procedures for simpler designs.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00276422
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    Paper (German National Licenses)
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  • 3
    Electronic Resource
    Electronic Resource
    Genetic diversity and differentiation of indica and japonica rice detected by RFLP analysis (1992)
    Springer
    Theoretical and applied genetics 83 (1992), S. 495-499 
    Details
    ISSN: 1432-2242
    Keywords: Oryza sativa ; Phenotypic diversity ; Differentiation ; Randomization test ; RFLP
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Genetic diversity and differentiation in indica and japonica groups of the cultivated rice (Oryza sativa L.) were studied by assaying DNA restriction fragment length polymorphisms of 12 indica and 14 japonica rice lines digested with three restriction endonucleases. A total of 49 probes were selected to represent the entire RFLP map at intervals of 20–30 cM. It was shown that 95 of the 145 possible probe/enzyme combinations, involving 43 probes and all three enzymes, detected restriction fragment length variation, and the degree of polymorphism varied greatly from one probe/enzyme combination to another. These results demonstrate that indica rice is genetically more diverse than japonica type. Significant differentiation between the two rice groups was detected by 33 probes representing 11 of the 12 rice chromosomes. It was deduced that the processes leading to differentiation involved a combination of molecular events that include base substitutions and insertion/deletions.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00226539
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    Paper (German National Licenses)
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  • 4
    Electronic Resource
    Electronic Resource
    Polymorphisms of α-amylase activity in barley landraces and cultivars from China (1990)
    Springer
    Euphytica 48 (1990), S. 245-251 
    Details
    ISSN: 1573-5060
    Keywords: Hordeum vulgare ; barley ; α-amylase activity ; analysis of variance ; covariance analysis ; China
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary α-Amylase activity was assayed by measuring reducing power equivalent for 80 accessions of cultivated barley, Hordeum vulgare L., representing major barley growing areas of China. Replications were applied at two different levels of the experiment and enzyme activity was assayed on four consecutive days starting on the 6th day after germination. The area under the curve formed by connecting the four data points was integrated as the measurement of α-amylase activity. The results established that there was extensive variation in α-amylase activity in cultivated barley; about three-fold difference existed among accessions assayed. Comparisons were also made between six- and two-rowed, and between covered and naked barleys. The results showed that high α-amylase activity was not necessarily associated with six-rowed type, and that covered barleys were slightly higher in α-amylase activity than naked ones.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00023657
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  • 5
    Electronic Resource
    Electronic Resource
    Mapping and genetic analysis of two fertility restorer loci in the wild-abortive cytoplasmic male sterility system of rice (Oryza sativa L.) (1997)
    Springer
    Euphytica 98 (1997), S. 183-187 
    Details
    ISSN: 1573-5060
    Keywords: fertility restoration ; hybrid rice ; linkage analysis ; Oryza sativa ; RFLP
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract Hybrid varieties developed by making use of the wild abortive cytoplasmic male sterility system account for 90% of hybrid rice produced. Previous inheritance studies have established that the fertility restoration in this system is controlled by two major loci, but the chromosomal locations of the fertility restorer (Rf) loci have yet to be resolved. In this study we determined the genomic locations of the two Rf loci by their linkage to molecular markers. The Rf gene containing regions were identified by surveying two bulks, made of 30 highly fertile and 46 highly sterile plants from a large F2 population of the cross between Zhenshan 97A and Minghui 63, with RFLP markers covering the entire rice genome. The survey identified two likely Rf gene containing regions, located on chromosomes 1 and 10 respectively. This was confirmed by ANOVA using a large random sample from the same F2 population and also with a genome-wide QTL analysis of a test-cross population. The results also showed that both loci have major effects of almost complete dominance on fertility restoration and the effect of the locus on chromosome 10 is larger than the one on chromosome 1. The two loci acted as a pair of classical duplicate genes; a single dominant allele at one of the two loci would suffice to restore the fertility to normal or nearly normal. Closely linked markers identified in this study may be used for marker assisted selection in hybrid rice breeding programs.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1023/A:1003165116059
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    Paper (German National Licenses)
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