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  • 1
    ISSN: 1435-0653
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: 2 populations. Two PSGMS loci, located on chromosomes 7 and 12, respectively, were identified in both crosses. The locus on chromosome 7 was the same as pms1, identified previously in a cross between an indica PSGMS line and an indica cultivar. The one on chromosome 12 was a new locus that we designated as pms3. Both loci had major effects on fertility and acted like a pair of classical duplicated genes in controlling male sterility. Comparison of the present finding with previous results indicated a complex genetic basis of PSGMS. The implications of such genetic complexity in breeding for PSGMS lines are also discussed.
    Type of Medium: Electronic Resource
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  • 2
    ISSN: 1435-0653
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Xa21 , a broad-spectrum BB resistance gene, into Minghui 63 by molecular marker-assisted selection (MAS). A polymerase chain reaction (PCR)-based MAS system was established consisting of a marker that is a part of Xa21, a marker located at 0.8 centimorgans (cM) from the Xa21 locus on one side, and a marker at 3.0 cM from the gene on the other side. A total of 128 restriction fragment length polymorphism (RFLP) markers, evenly distributed on the 12 chromosomes, were used to recover the genetic background of Minghui 63. The resulting improved version of Minghui 63, or `Minghui 63(Xa21)', was exactly the same as the original except for a fragment of less than 3.8 cM in length surrounding the Xa21 locus. Both Minghui 63(Xa21) and its hybrid with `Zhenshan 97A' referred to as `Shanyou 63(Xa21)' showed the same spectrum of BB resistance as the donor parent. Field examination of a number of agronomic traits showed that Minghui 63(Xa21) and Shanyou 63(Xa21) were identical to Minghui 63 and Shanyou 63, when there was no disease stress. Under heavily diseased conditions, Minghui 63(Xa21) showed significantly higher grain weight and spikelet fertility than Minghui 63, and Shanyou 63(Xa21) was significantly higher than Shanyou 63 in grains per panicle, grain weight, and yield.
    Type of Medium: Electronic Resource
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  • 3
    ISSN: 1573-5060
    Keywords: fertility restoration ; hybrid rice ; linkage analysis ; Oryza sativa ; RFLP
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract Hybrid varieties developed by making use of the wild abortive cytoplasmic male sterility system account for 90% of hybrid rice produced. Previous inheritance studies have established that the fertility restoration in this system is controlled by two major loci, but the chromosomal locations of the fertility restorer (Rf) loci have yet to be resolved. In this study we determined the genomic locations of the two Rf loci by their linkage to molecular markers. The Rf gene containing regions were identified by surveying two bulks, made of 30 highly fertile and 46 highly sterile plants from a large F2 population of the cross between Zhenshan 97A and Minghui 63, with RFLP markers covering the entire rice genome. The survey identified two likely Rf gene containing regions, located on chromosomes 1 and 10 respectively. This was confirmed by ANOVA using a large random sample from the same F2 population and also with a genome-wide QTL analysis of a test-cross population. The results also showed that both loci have major effects of almost complete dominance on fertility restoration and the effect of the locus on chromosome 10 is larger than the one on chromosome 1. The two loci acted as a pair of classical duplicate genes; a single dominant allele at one of the two loci would suffice to restore the fertility to normal or nearly normal. Closely linked markers identified in this study may be used for marker assisted selection in hybrid rice breeding programs.
    Type of Medium: Electronic Resource
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  • 4
    ISSN: 1573-5060
    Keywords: adaptedness ; morphological and genetic differentiation ; Oryza sativa L. ; RFLP
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract Indica and Japonica differentiation under the species of Oryza sativa L. has been documented using morphological characters, isozyme and DNA markers. We illustrate marker assisted analysis of genetic differentiation with a sample of RFLP and isozyme data from 111 accessions of rice lines and compare it to morphological differentiation based on Cheng and Wang' morphological character index. The results lead to three main conclusions: (1) Among the 92 polymorphic cloned probes deliberately selected, 41 were highly associated with Indica-Japonica differentiation, suggesting such differentiation was an accumulative process of gene change at multiple loci; (2) There exist two main types of relationships between morphological and genetic differentiation. For well differentiated Indica and Japonica (as the case in the control subset), morphology and genomic constitutions are synchronously specialized. For incompletely differentiated rice lines (as the case in the subset of DH lines), morphological differentiation and genetic differentiation are asynchronous. (3)Synthetic or incompletely differentiated rice lines with different genomic constitutions are often adapted to specific environments. These results indicate that marker alleles provide applied breeders with effective ways to identify, track, and incorporate regions of chromosomes governing adaptedness to specific target environments into improved cultivars.
    Type of Medium: Electronic Resource
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