Library

feed icon rss

Your email was sent successfully. Check your inbox.

An error occurred while sending the email. Please try again.

Proceed reservation?

Export
Filter
  • Genotypisierung  (1)
  • MRSA strains  (1)
  • Schlüsselwörter Enterokokken  (1)
  • 1
    Electronic Resource
    Electronic Resource
    Springer
    Bundesgesundheitsblatt, Gesundheitsforschung, Gesundheitsschutz 42 (1999), S. 847-853 
    ISSN: 1437-1588
    Keywords: Schlüsselwörter Enterokokken ; Glycopeptidresistenz ; Ausbrüche ; Genotypisierung ; Key words Enterococci ; Glycopeptide resistance ; Outbreaks ; Genotyping
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Description / Table of Contents: Summary Ninety-two strains of glycopeptide-resistant enterococci (GRE) from 80 patients of 31 hospitals in 11 federal states were analyzed. They were primarily isolated in intensive care units (general, surgical, internistic, pediatric, oncological ones), but also in nephrologic/dialysis or neurologic/orthopaedic wards. A dominance of VanA type E. faecium strains was observed (n=81; 88.0%). In addition, strains of E. faecalis (VanA; n=4; 4.4%), E. faecium (VanB; n=1; 1.1%), and E. gallinarum (VanC1, n=6; 6.5%) were found. The 81 VanA strains of E. faecium possessed the following resistances to other antibiotics: erythromycin, ciprofloxacin (both 93.8%), ampicillin, oxytetracycline (both 88.9%), rifampicin (79.0%), trimethoprim/sulfameracin (61.7%), chloramphenicol (18.9%), fusidic acid (12.3%), quinupristin/dalfopristin (7.4%); gentamicin (46.9%) and streptomycin (37.0%), also together with ampicillin resistance (9.9–35.8%). In some of these VanA type strains resistance to teicoplanin cannot be completely expressed in-vitro. Macrorestriction analysis of E. faecium outbreak isolates (VanA type) from hospitals in different federal states indicated an intra- and interhospital spread of a defined strain. However, heterogeneous plasmid and plasmid restriction patterns showed that these strains were not completely identical.
    Notes: Zusammenfassung Es wurden 92 Stämme Glycopeptid-resistenter Enterokokken (GRE) von 80 Patienten aus 31 Krankenhäusern in elf Bundesländern analysiert. GRE wurden vorrangig in den intensivmedizinischen Bereichen (allgemeine, chirurgische, internistische, pädiatrische, onkologische ITS-Stationen), aber auch in Nephrologie/Dialyse- oder Neurologie/Orthopädie-Abteilungen isoliert. Dabei war die Dominanz von VanA-E. faecium-Stämmen zu beobachten (n=81; 88,0%), weiterhin wurden Stämme von E. faecalis (VanA; n=4; 4,4%), E. faecium (VanB; n=1; 1,1%) sowie E. gallinarum (VanC1; n=6; 6,5%) gefunden. Die 81 VanA-Stämme von E. faecium zeigten folgende Resistenzquoten gegen weitere Antibiotika: Erythromycin, Ciprofloxacin (jeweils 93,8%), Ampicillin, Oxytetracyclin (je 88,9%), Rifampicin (79,0%), Trimethoprim/Sulfamerazin (61,7%), Chloramphenicol (18,9%), Fusidinsäure (12,3%), Quinupristin/Dalfopristin (7,4%); Gentamicin (46,9%) und Streptomycin (37,0%), auch kombiniert mit Ampicillinresistenz (9,9–35,8%). Bei einigen dieser VanA-Stämme war die Resistenz gegen Teicoplanin in-vitro nicht vollständig exprimiert. Gleiche Makrorestriktionsmuster von E. faecium-Ausbruchsstämmen (VanA-Typ) aus Krankenhäusern in verschiedenen Bundesländern deuteten auf eine intra- und interhospitale Verbreitung eines definierten GRE-Stammes. Analysen der Plasmid- und Plasmidrestriktionsmuster dieser Isolate zeigten jedoch deutliche Unterschiede. Dies bedeutet, daßE. faecium-Isolate mit gleichem Makrorestriktionsmuster nicht unbedingt identisch sein müssen.
    Type of Medium: Electronic Resource
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
  • 2
    ISSN: 1573-7284
    Keywords: Genomic DNA fragments ; MRSA strains
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract The analysis of genomic DNA fragment patterns has revealed as a powerful tool for strain discrimination inStaphylococcus aureus; for use as an epidemiological marker, stability during the course of an outbreak is an essential prerequisite. Genomic DNA fragment patterns (SmaI restriction, pulsed-field electrophoresis) of four different epidemic MRSA strains were compared along with intra- and interhospital and country-wide spread over more than 12 months in Germany. Strain I was isolated from infections in 8 hospitals. In one hospital a subclone arised which differed from the original strain by 4 fragments. Strain II was spread among 4 hospitals, isolates from three of these hospitals exhibited a variability of one to three fragments in the 150–200 kb range. Two hospitals in the Hannover-area were affected by strain III; in 17 isolates of this strain a variability up to three fragments was found in the 170–200 kb range. Strain IV was isolated from 19 cases of infections in 3 hospitals in Berlin. The fragment patterns were completely stable. When S. aureus strains are typed by genomic DNA fragment patterns, a variability in a definite range of molecular masses during the course of an epidemic should be taken into consideration.
    Type of Medium: Electronic Resource
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
Close ⊗
This website uses cookies and the analysis tool Matomo. More information can be found here...